##gff-version 3
##sequence-region BBa_K2120419 1 1816
BBa_K2120419	.	ribosome_entry_site	831	842	.	+	0	ID=BBa_K1045010;Name=BBa_K1045010
BBa_K2120419	.	non_covalent_binding_site	831	842	.	+	0	ID=annotation2360435;Name=inverse RBS;Parent=BBa_K1045010
BBa_K2120419	.	terminator	1688	1816	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2120419	.	terminator	1688	1767	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2120419	.	engineered_region	1688	1767	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2120419	.	stem_loop	1699	1742	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2120419	.	terminator	1776	1816	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2120419	.	engineered_region	1776	1816	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2120419	.	stem_loop	1783	1802	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2120419	.	polyA_site	1803	1816	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2120419	.	stop_codon	1809	1809	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2120419	.	promoter	894	947	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2120419	.	promoter	913	918	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2120419	.	non_covalent_binding_site	919	937	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2120419	.	engineered_region	894	947	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2120419	.	non_covalent_binding_site	894	912	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2120419	.	promoter	936	941	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2120419	.	terminator	1	129	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_K2120419	.	engineered_region	50	129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_K2120419	.	engineered_region	1	41	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_K2120419	.	CDS	974	1679	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2120419	.	engineered_tag	1655	1679	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2120419	.	CDS	974	1648	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2120419	.	ribosome_entry_site	956	967	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2120419	.	conserved_region	960	963	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K2120419
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagcggacccactttcacatttaagttgtttttctaatcc
gcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaat
aatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaac
ctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaa
ttgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagt
aaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtat
ggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctg
ctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctg
ttaatcactttacttttatctaatctggacattactagagtttctcctcttttactagaggctagcacagtccctagga
ctgagctagctgtaaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcact
actagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatg
gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgcta
aactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc
ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatg
aacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaac
tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtat
gtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaa
gttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacct
cccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata