LOCUS       BBa_K2120421            1902 bp    DNA     linear       10-Jun-2024
DEFINITION  B0015+cI+B0034+J23119+pR+B0032+mRFP+B0015
ACCESSION   BBa_K2120421
VERSION     BBa_K2120421.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..912
                     /label=BBa_K2120105
     misc_feature    921..932
                     /label=inverse RBS
     RBS             921..932
                     /label=BBa_K1045010
     promoter        941..975
                     /label=promoter
     promoter        941..975
                     /label=BBa_K2120101
     promoter        1021..1026
                     /label=-10
     promoter        998..1003
                     /label=-35
     misc_feature    1008..1024
                     /label=OR1
     misc_feature    984..1000
                     /label=OR2
     misc_feature    984..1032
                     /label=BBa_R0051
     promoter        984..1032
                     /label=BBa_R0051
     RBS             1041..1053
                     /label=RBS-3\Weak
     RBS             1047..1050
                     /label=RBS
     misc_feature    1041..1053
                     /label=BBa_B0032
     RBS             1041..1053
                     /label=BBa_B0032
     CDS             1060..1734
                     /label=mrfp1
     misc_feature    1741..1765
                     /label=Help:Barcodes
     CDS             1060..1765
                     /label=BBa_E1010
     stem_loop       1785..1828
                     /label=stem_loop
     misc_feature    1774..1853
                     /label=BBa_B0010
     terminator      1774..1853
                     /label=BBa_B0010
     stem_loop       1869..1888
                     /label=T7 TE
     misc_feature    1895..1895
                     /label=stop
     polyA_site      1889..1902
                     /label=polya
     misc_feature    1862..1902
                     /label=BBa_B0012
     terminator      1862..1902
                     /label=BBa_B0012
     terminator      1774..1902
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt
      241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg
      301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag
      361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat
      421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc
      481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc
      541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg
      601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat
      661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa
      721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg
      781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt
      841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt
      901 ttttgtgctc attactagag tttctcctct tttactagag gctagcatta tacctaggac
      961 tgagctagct gtcaatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt
     1021 gataatggtt gctactagag tcacacagga aagtactaga tggcttcctc cgaagacgtt
     1081 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
     1141 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
     1201 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     1261 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     1321 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
     1381 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
     1441 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
     1501 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
     1561 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     1621 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
     1681 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
     1741 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc
     1801 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1861 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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