BBa_K2121002 1 BBa_K2121002 Lambda_Beta_B5ZVM8_RHILW (red_rhizo-MAGE) 2016-10-19T11:00:00Z 2016-10-20T09:46:57Z Gene Rleg2_2465 phage for Rhizobium leguminosarum bv. trifolii (strain WSM2304) Coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (R. leg). Within Yale iGEM 2016, known as gBlock 2, amplified for cloning with primers priG 449, 450. Uniprot KB B5ZVM8_RHILW. E-value 0.00041 false false _2590_ 33231 33231 9 false Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti 1021 and R. tropici CIAT 899. false Jinny van Doorn annotation2525731 1 bet recombination protein cds range2525731 1 1 1005 BBa_K2121002_sequence 1 atgaacgcaatgacccgctatgacatgacgccgaagcagatttcgctcgtcaaaacgacgatcgcgaaggactgcaacgatgaagagtttaatctgttttgcgaagtcgcccgcgcgaagggacttgatccctttctcgggcagatcatcccgatggtcttttcgaagggcgatgccgaaaaacggaagatgactatcatcatcacccgcgacggccagcgcgtgattgctcaacgctgcggcgactatcggccggccagcaagccgacacagttcgaagtcgacaaggcgatgatggcgccgaccaatccgctcggcatcgtctcggcaacggtctacctgtggaagcgcgatccgaaaaccggcgagtggttcgaagtcgtcggccaggcctattgggatgagttcgcgccgatcaagcgcaaggccgccggcggatacaagtgggaggacactggcgaggtctggcccgatagcggcaagccgaaaaggaagaaggtggccgtcggcgaggaaacggaagtcctcgacgattccggcaactggtgccgcatgccacgcctgatgattgagaagtgtgctcagatgcaggcacttcgcgccggctggccggaagaatttaccggcacctatgacgaggccgaaatggaccgcgccaaggttctcgacctgacggcttccgagattgccgcccaggagcagcaggacaaccggctgcgcgcgatcggcggcaaggacgccctcaccatttggtggagcgaaggcttcggtctcgaaaacgttcctgatggaaagttcttcgatcgcgtcatcgaccatatcgccaatctcgatgcgaccgccgtggcgaagtgggaagacgcgaaccgcgctagcctgcaaatttattgggctcgtcatcccggcgacgccctcgaactgaagaagcgcatcgaggctgcacgccagaagcgcgcggcgaacgtcgacaaggcgctgcaacagcatccgttgatggcaggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z