BBa_K2121003 1 BBa_K2121003 SPP1_GP35_Q38143 2016-10-19T11:00:00Z 2016-10-20T10:40:37Z Sequence ID: X97918.2, SPP1_GP35_Q38143 Coding sequence for beta recombinase protein homolog. For use in recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (ie S. meliloti and CIAT 899). Identified with BLASTp from homology to the SPP1 35 (Q38143) B. subtillus phage beta-like recombination protein. Derived from phage that selectively infects R. Within Yale iGEM 2016, known as gBlock 14, amplified for cloning with primers priG 459, 460. Uniprot SPP1_GP35_Q38143 . E-value 4.90E-12 false false _2590_ 33231 33231 9 false Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti. false Jinny van Doorn annotation2525734 1 cds SPP1 page recombination protein range2525734 1 1 864 BBa_K2121003_sequence 1 atggcaactaaaaaacaagaggaattaaaaaacgcactagcacaacaaaacggagccgttccgcaaacaccagtcaagccgcaggataaagtaaagggctatctcgaaagaatgatgccagctattaaagatgtgttgcctaagcatttagacgctgatcgactttcccgcatcgcaatgaacgtcattaggactaacccgaaacttctggaatgtgatacggcatcactcatgggggctgtgctggagtcagctaaattaggagtggagccgggacttttgggacaagcgtatattttgccgtacacgaattacaaaaagaaaacggttgaagctcagttcattcttggttataagggcctccttgatctcgtgagacgctctggacacgtgagcacaatcagcgctcaaactgtttacaaaaacgacacattcgaatatgaatatgggttagatgacaagcttgttcacagacccgcaccattcggaacagacagaggggagccagttggatactacgctgtagcgaaaatgaaggatggcggatataacttccttgtcatgagtaaacaggatgtcgagaaacaccgggacgccttttccaagtctaaaaacagagagggagtagtgtatggcccatgggctgaccacttcgacgcaatggcgaaaaagacagtcttgcgccagcttatcaactatttacctatcagtgttgagcaattatcaggagtagcggcagacgaacggacaggatcagaacttcataaccaatttgcggatgacgacaacattattaacgtcgatatcaatacaggtgaaatcattgaccatcaagaaaaattaggaggggaaacaaatgaatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z