BBa_K2121003
1
BBa_K2121003
SPP1_GP35_Q38143
2016-10-19T11:00:00Z
2016-10-20T10:40:37Z
Sequence ID: X97918.2, SPP1_GP35_Q38143
Coding sequence for beta recombinase protein homolog.
For use in recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (ie S. meliloti and CIAT 899). Identified with BLASTp from homology to the SPP1 35 (Q38143) B. subtillus phage beta-like recombination protein. Derived from phage that selectively infects R.
Within Yale iGEM 2016, known as gBlock 14, amplified for cloning with primers priG 459, 460.
Uniprot SPP1_GP35_Q38143 . E-value 4.90E-12
false
false
_2590_
33231
33231
9
false
Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti.
false
Jinny van Doorn
annotation2525734
1
cds SPP1 page recombination protein
range2525734
1
1
864
BBa_K2121003_sequence
1
atggcaactaaaaaacaagaggaattaaaaaacgcactagcacaacaaaacggagccgttccgcaaacaccagtcaagccgcaggataaagtaaagggctatctcgaaagaatgatgccagctattaaagatgtgttgcctaagcatttagacgctgatcgactttcccgcatcgcaatgaacgtcattaggactaacccgaaacttctggaatgtgatacggcatcactcatgggggctgtgctggagtcagctaaattaggagtggagccgggacttttgggacaagcgtatattttgccgtacacgaattacaaaaagaaaacggttgaagctcagttcattcttggttataagggcctccttgatctcgtgagacgctctggacacgtgagcacaatcagcgctcaaactgtttacaaaaacgacacattcgaatatgaatatgggttagatgacaagcttgttcacagacccgcaccattcggaacagacagaggggagccagttggatactacgctgtagcgaaaatgaaggatggcggatataacttccttgtcatgagtaaacaggatgtcgagaaacaccgggacgccttttccaagtctaaaaacagagagggagtagtgtatggcccatgggctgaccacttcgacgcaatggcgaaaaagacagtcttgcgccagcttatcaactatttacctatcagtgttgagcaattatcaggagtagcggcagacgaacggacaggatcagaacttcataaccaatttgcggatgacgacaacattattaacgtcgatatcaatacaggtgaaatcattgaccatcaagaaaaattaggaggggaaacaaatgaatag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z