BBa_K2121004 1 BBa_K2121004 Lambda_Beta_F7X3D1 (red_rhizo-MAGE) 2016-10-19T11:00:00Z 2016-10-20T10:47:07Z Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11). Gene: bet, Ordered Locus Names:SM11_chr1058 Coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11) Within Yale iGEM 2016, known as gBlock 15, amplified for cloning with primers priG 461, 462 Unitprot KB F7X3D1_SINMM, E value 1.50E-36 false false _2590_ 33231 33231 9 false Elimination of any illegal restriction sites, and codon optimization for expression. false Jinny van Doorn annotation2525738 1 cds recombination protein bet F7X3D1 range2525738 1 1 951 BBa_K2121004_sequence 1 atgaaccagcttgcaaaagcgccgcgccggtctgtgcttgtcgatatggccgcgcacttcggcatggaggccgatgccttcgaaatgacggtgcgggcgcaatgctcgccgaccccaaagaagggcgagcaattccgtccgctgacccgcgaggagtttgccgccttcctgctggtcgccaagaaatacgatctcaatccgctaacccgcgagattttcgcctatccgaagcgcggcggcggcgtcgtgccgatcgtctcgattgacgggtggatcaacctcgtcaattcgcacccggcttgcgacggctttgagttcacgtgggagcgcgatgccaacggcgatccgatttcttgcacatgcatcatgcatcgcaaggaccgaagccacccgaccgccgtcaccgaatacctcgccgagtgctggcgcgacaccgaaccgtggaagatgaagcaccgcatgttgcgccacaaggcgctgatgcaatgcgcccgctatgccttcggctttgccggcatctacgacgaggacgagggccggcggatcgccgaagatcagaacattgccctattgccgccagcgccgcgcgcgccgcgcataggtcagcagagccctgctggtgaaaaaatccagacggcgcagggcgatgcgacggaggtcgagtccgggaccgggcagccgccggtagactcggcctccccccttgatgacgagcccgaccccgatcgcgaggaaataggcggcgtcgatgccgactccaccccggacgcggagttctttgacgaactgcgcgaccggctggccgaggcgaaagacgccgccagcgtggaggaaatctggacagagcttgacccgatggcgcggttcgaaggctccgaccttgatcaagagatttgccagaagatcaaagcgcgccggctgcgcgatctggaaaaggaggatgggaaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z