LOCUS BBa_K2121005 8073 bp DNA linear 17-Dec-2024 DEFINITION Taurine Inducible Promoter ACCESSION BBa_K2121005 VERSION BBa_K2121005.1 FEATURES Location/Qualifiers misc_feature 57..84 /label=tpr terminator CDS 116..536 /label=putative transcriptional regulator protein CDS 693..728 /label=SMb21526; putative taurine uptake ABC transporter misc_feature 729..740 /label=LIC Upstream Site CDS 741..1460 /label=citrine misc_feature 1461..1474 /label=LIC Downstream Site misc_feature 1511..1579 /label=T1 terminator CDS 2131..2817 /label=ORF1 CDS 3116..3745 /label=pVS1 staA misc_feature 4020..4614 /label=ORF 2 misc_feature 1040..5274 /label=pVS1 repA misc_feature 6162..6781 /label=pBR322 Origin misc_feature 7013..7945 /label=specR misc_feature 7013..8012 /label=spect ORIGIN 1 cctggccttt tgctggcctt ttgctcaagc ttgcatgcct gcaggtcgag atccccagcc 61 cgcctaatga gcgggctttt ttttggtcga caccggtcac gtgcctagga ctagtggtcc 121 atctgaccgt ataggaacgg ataagggtag gttcgccagt cgaggggctt acgcatctgc 181 ttggccacga tgaaggtcga gcgcaacttg gccgaccagt cgatggcatc ggccgcagca 241 tgggcggcct gcatcgcgat ggctgtcgtt gggggcttgc cggccacacg gtagccgctc 301 cggttcgccg cctccacata gccctgggaa gcgagttcct gataggcgag cgttacggtg 361 atccgcgaaa tattcagata ggccgcaagc ttgcggctgg agggcaattg ggcgcccggc 421 gccaggcggc ccgacaatac ggccgaaacg atcgtttcgc ggatctgcgc ctgcaggccc 481 gtttggctgg cccggtcaag gaaaaagatc gtttcggaga cggtcgagcc cgccattgtt 541 cacctcgccc aactggactc atctaacttc ctatctggat ataaggcaat ccgaagcgtg 601 ggcctatttc tattcagcaa gcaaagaccg tcattcaatg tacggcgcga ccccgcaaaa 661 agcggggcga cataaccaaa gagggtaacg acatgatcca ccttagaagt ttcaagcact 721 tgtccggata cttccaatcc atggtgagca agggcgagga gctgttcacc ggggtggtgc 781 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 841 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 901 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc 961 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 1021 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 1081 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 1141 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 1201 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 1261 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 1321 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg 1381 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 1441 tggacgagct gtacaagtaa agtaaaggtg gataaagctt gatgggggat cccatggtac 1501 gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 1561 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag 1621 gcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 1681 aatcagggga taacgcagga gcttagcttg agcttggatc agattgtcgt ttcccgcctt 1741 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc 1801 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt 1861 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttaaa gtactttaaa 1921 gtactttaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt 1981 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct 2041 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat 2101 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg 2161 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac 2221 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg 2281 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta 2341 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc 2401 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc 2461 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc 2521 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc 2581 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg 2641 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc 2701 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg 2761 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac 2821 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga 2881 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa 2941 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag 3001 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg 3061 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa 3121 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct 3181 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg 3241 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga 3301 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga 3361 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct 3421 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt 3481 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc 3541 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc 3601 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac 3661 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc 3721 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca 3781 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc 3841 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc 3901 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac 3961 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc 4021 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat 4081 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa 4141 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg 4201 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc 4261 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct 4321 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag 4381 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc 4441 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga 4501 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg 4561 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc 4621 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca 4681 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac 4741 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag 4801 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag 4861 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg 4921 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt 4981 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag 5041 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc 5101 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat 5161 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc 5221 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta 5281 gcaggggaaa aaggtcgaaa acgtctcttt cctgtggata gcacgtacat tgggaaccca 5341 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac 5401 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac 5461 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag 5521 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc 5581 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca 5641 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat 5701 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 5761 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 5821 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 5881 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 5941 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc 6001 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 6481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 6721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6841 catgatatat ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca 6901 gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag 6961 ttaacgccgc gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga cattatttgc 7021 cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc 7081 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg 7141 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga 7201 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat 7261 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata 7321 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc 7381 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga 7441 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg 7501 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa 7561 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg 7621 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca 7681 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc 7741 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc 7801 ccatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc 7861 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag 7921 actgtacccc aaaaaaacat gtcataacaa gaagccatga aaaccgccac tgcgccgtta 7981 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat 8041 tacagcttac gaaccgaaca ggcttatgtc cac //