LOCUS BBa_K2121007 8114 bp DNA linear 22-Dec-2024 DEFINITION Rhamnose Inducible Promoter ACCESSION BBa_K2121007 VERSION BBa_K2121007.1 FEATURES Location/Qualifiers misc_feature 52..79 /label=trp terminator CDS 111..526 /label=short chain dehydrogenase CDS 721..771 /label=SMc02323; PUTATIVE TRANSCRIPTION REGULATOR PROTEIN misc_feature 772..786 /label=LIC Upstream site CDS 794..1512 /label=citrine misc_feature 1521..1535 /label=LIC Downstream Site misc_feature 1572..1640 /label=T1 terminator CDS 2192..2878 /label=ORF 1 CDS 3177..3806 /label=pVS1 staA CDS 4081..4675 /label=ORF 2 CDS 4201..5308 /label=pVS1 repA misc_feature 6223..6842 /label=pBR322 origin CDS 7074..8006 /label=specR CDS 7074..8073 /label=spect ORIGIN 1 ccttttgctg gccttttgct caagcttgca tgcctgcagg tcgagatccc cagcccgcct 61 aatgagcggg cttttttttg gtcgacaccg gtcacgtgcc taggactagt acatgcggct 121 tcggaacata ggcgtgcagc ggcgtgtcga tcgaggcggc gcgggggttg aggttgaaag 181 tgcaatgggg caggtagccg accatctcgt cttcgaattc gacgccgcga tagatgccct 241 tcagcgcgcg gagcttgtcc atgtagaggg tggagaagcc atccatcttg atcgtgccga 301 cgtcgccgcc ggagcccttg acccagagga cctcgacaat ttcgccggtc agcggatcct 361 tttccatgac cttggcggag gtattgccgc cgccgtaatt ggtgatccgc ttgtccgatc 421 cgaggaggtt cgaacggtag aggagctttt ccggctcggt cattcctgcc gccttgccgt 481 cgtcccaaag attggcaagg cgcgcgccct ggtgcttgtc gagcatgtcc aactcctccc 541 gctgatctgg ctgccgcaac atgaaccgcg gccatatgat tggcaccaga tcacacggtg 601 attacgcgga tgtcaatcaa aaacgatcaa aaacgttcat gatgcgccgc acaatggaaa 661 aacatgattg attgtgattg acaatgctcg gaatggatgg aagcatgcgg agcatcggag 721 gagcgcatgc acgagaaaga aagacacagg atcatcctgt cggcggtgca gtacttccaa 781 tccatggaga ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 841 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 901 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 961 ccctggccca ccctcgtgac caccttcggc tacggcctga tgtgcttcgc ccgctacccc 1021 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 1081 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 1141 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1201 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1261 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1321 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1381 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc 1441 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1501 ctgtacaagt aaacggtctc cagtaaaggt ggataaagct tgatggggga tcccatggta 1561 cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1621 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta 1681 ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 1741 gaatcagggg ataacgcagg agcttagctt gagcttggat cagattgtcg tttcccgcct 1801 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag 1861 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt 1921 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttaa agtactttaa 1981 agtactttaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg 2041 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc 2101 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa 2161 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct 2221 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca 2281 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc 2341 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct 2401 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc 2461 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg 2521 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg 2581 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac 2641 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt 2701 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg 2761 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt 2821 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa 2881 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg 2941 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa 3001 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa 3061 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat 3121 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga 3181 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc 3241 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg 3301 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg 3361 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg 3421 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc 3481 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg 3541 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg 3601 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc 3661 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca 3721 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg 3781 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac 3841 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag 3901 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac 3961 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata 4021 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg 4081 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca 4141 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca 4201 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc 4261 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc 4321 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc 4381 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa 4441 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac 4501 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg 4561 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc 4621 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac 4681 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc 4741 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga 4801 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa 4861 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa 4921 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat 4981 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt 5041 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa 5101 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt 5161 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga 5221 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat 5281 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct 5341 agcaggggaa aaaggtcgaa aacgtctctt tcctgtggat agcacgtaca ttgggaaccc 5401 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa 5461 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa 5521 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca 5581 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc 5641 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc 5701 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca 5761 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 5821 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 5881 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 5941 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 6001 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 6061 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6421 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6661 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 6721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 6781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6901 gcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc 6961 agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct aatcgcttga 7021 gttaacgccg cgccgcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg 7081 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg 7141 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg 7201 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg 7261 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca 7321 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat 7381 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg 7441 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg 7501 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct 7561 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga 7621 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc 7681 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc 7741 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg 7801 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc 7861 cccatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc 7921 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata 7981 gactgtaccc caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt 8041 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca 8101 ttacagctta cgaa //