LOCUS BBa_K2121008 8140 bp DNA linear 29-Nov-2024 DEFINITION Melibiose Inducible Promoter ACCESSION BBa_K2121008 VERSION BBa_K2121008.1 FEATURES Location/Qualifiers misc_feature 57..84 /label=trp terminator CDS 116..456 /label=alpha-galactoside utilization CDS 652..777 /label=alpha-galactosidase (melibiase) protein misc_feature 778..792 /label=LIC Upstream Site CDS 799..1518 /label=citrine_ORF misc_feature 1527..1541 /label=LIC Downstream Site misc_feature 1578..1646 /label=T1 terminator CDS 2198..2884 /label=ORF 1 CDS 3183..3812 /label=pVS1 staA CDS 4087..4681 /label=ORF 2 CDS 4207..5314 /label=pVS1 repA misc_feature 6229..6848 /label=pBR322 origin CDS 7080..8012 /label=specR CDS 7080..8079 /label=spect ORIGIN 1 cctggccttt tgctggcctt ttgctcaagc ttgcatgcct gcaggtcgag atccccagcc 61 cgcctaatga gcgggctttt ttttggtcga caccggtcac gtgcctagga ctagttcctc 121 gccgttcatg gagatccaga agaattccca gcgcccgcct tcctccagcc aatagcggtg 181 attgtggggg acgagcagga gcagcgtctc gcccggacgc acgcggtggg tgcggttttc 241 atatcgcagg ttgccggcgc cgccgatcgt gtgctgcagg acggtgaagg gcgtctgacc 301 gcgcttgcgg ccgtcccaat cgtagcttgc atcggtcctg atctcgtagc cggtacttgt 361 cggcatcgtg tgcaggctct ggcgtccccg cggcagtgag acagtccgca tcgccggtcc 421 ccgatcgatc agttgctgca gcacagaatt acccatgaaa gcacaatacc tctctagtcg 481 cgcacccgat tatacacata atgcccccag atcgcgcaag acatacggtt atcgcggcgt 541 ttgacgaggg gttggcgagg aggatgccgc attgccgatc ccggtcaggc caatgaactg 601 attttaccct tttagcgcgt aggtcgcagt ctgaggagga atagcacggc catggccagc 661 ttcaagatcg ccatcatcgg cgccggcagc atcggcttca ccaagaagct cttcaccgac 721 attctttccg tgccggagct ccgggacgtc gagttcgcgt tgacggatct gagcgagtac 781 ttccaatcca tggagaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 841 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 901 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 961 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc 1021 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 1081 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 1141 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1201 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 1261 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 1321 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 1381 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 1441 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 1501 gacgagctgt acaagtaaac ggtctccagt aaaggtggat aaagcttgat gggggatccc 1561 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 1621 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta 1681 gacctaggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 1741 tccacagaat caggggataa cgcaggagct tagcttgagc ttggatcaga ttgtcgtttc 1801 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga 1861 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg 1921 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttaaagta 1981 ctttaaagta ctttaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt 2041 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg 2101 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa 2161 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg 2221 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga 2281 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga 2341 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac 2401 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca 2461 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc 2521 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat 2581 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg 2641 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg 2701 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact 2761 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga 2821 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc 2881 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg 2941 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg 3001 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc 3061 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg 3121 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac 3181 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa 3241 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc 3301 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg 3361 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag 3421 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact 3481 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg 3541 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt 3601 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg 3661 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg 3721 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc 3781 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa 3841 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt 3901 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga 3961 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc 4021 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt 4081 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat 4141 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc 4201 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc 4261 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc 4321 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc 4381 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat 4441 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt 4501 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga 4561 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc 4621 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca 4681 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt 4741 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa 4801 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac 4861 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg 4921 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga 4981 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga 5041 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc 5101 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg 5161 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga 5221 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa 5281 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat 5341 tgccctagca ggggaaaaag gtcgaaaacg tctctttcct gtggatagca cgtacattgg 5401 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat 5461 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc 5521 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc 5581 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct 5641 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct 5701 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg 5761 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 5821 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 5881 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 5941 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 6001 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 6061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 6301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6661 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6901 ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat 6961 aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaatc 7021 gcttgagtta acgccgcgcc gcgaagcggc gtcggcttga acgaatttct agctagacat 7081 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga 7141 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt 7201 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc 7261 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt 7321 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc 7381 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag 7441 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct 7501 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc 7561 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg 7621 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct 7681 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt 7741 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg 7801 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc 7861 gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg 7921 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga 7981 ggcatagact gtaccccaaa aaaacatgtc ataacaagaa gccatgaaaa ccgccactgc 8041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta 8101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac //