BBa_K2121002 1 BBa_K2121002 Lambda_Beta_B5ZVM8_RHILW (red_rhizo-MAGE) 2016-10-19T11:00:00Z 2016-10-20T09:46:57Z Gene Rleg2_2465 phage for Rhizobium leguminosarum bv. trifolii (strain WSM2304) Coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (R. leg). Within Yale iGEM 2016, known as gBlock 2, amplified for cloning with primers priG 449, 450. Uniprot KB B5ZVM8_RHILW. E-value 0.00041 false false _2590_ 33231 33231 9 false Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti 1021 and R. tropici CIAT 899. false Jinny van Doorn annotation2525731 1 bet recombination protein cds range2525731 1 1 1005 BBa_K2121010 1 BBa_K2121010 Rhamnose_Lambda_Beta_B5ZVM8 2016-10-20T11:00:00Z 2016-10-21T12:55:47Z Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search. Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med). false false _2590_ 30243 30243 9 false Expression fusion with native S. meliloti protein. false Leslie Wang component2527482 1 BBa_K2121007 component2527484 1 BBa_K2121002 annotation2527482 1 BBa_K2121007 range2527482 1 1 45 annotation2527484 1 BBa_K2121002 range2527484 1 52 1056 BBa_K2121007 1 BBa_K2121007 Rhamnose Inducible Promoter 2016-10-20T11:00:00Z 2016-10-29T09:03:27Z Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1. Promoter regions in S. meliloti inducible by rhamnose. false false _2590_ 30243 30243 9 false Utilize native activation mechanism in S. meliloti. false Leslie Wang annotation2531255 1 pBR322 origin range2531255 1 6223 6842 annotation2531246 1 SMc02323; PUTATIVE TRANSCRIPTION REGULATOR PROTEIN range2531246 1 721 771 annotation2531252 1 pVS1 staA range2531252 1 3177 3806 annotation2531244 1 trp terminator range2531244 1 52 79 annotation2531257 1 spect range2531257 1 7074 8073 annotation2531249 1 LIC Downstream Site range2531249 1 1521 1535 annotation2531248 1 citrine range2531248 1 794 1512 annotation2531245 1 short chain dehydrogenase range2531245 1 111 526 annotation2531254 1 pVS1 repA range2531254 1 4201 5308 annotation2531247 1 LIC Upstream site range2531247 1 772 786 annotation2531251 1 ORF 1 range2531251 1 2192 2878 annotation2531256 1 specR range2531256 1 7074 8006 annotation2531250 1 T1 terminator range2531250 1 1572 1640 annotation2531253 1 ORF 2 range2531253 1 4081 4675 BBa_K2121010_sequence 1 atgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtactagatgaacgcaatgacccgctatgacatgacgccgaagcagatttcgctcgtcaaaacgacgatcgcgaaggactgcaacgatgaagagtttaatctgttttgcgaagtcgcccgcgcgaagggacttgatccctttctcgggcagatcatcccgatggtcttttcgaagggcgatgccgaaaaacggaagatgactatcatcatcacccgcgacggccagcgcgtgattgctcaacgctgcggcgactatcggccggccagcaagccgacacagttcgaagtcgacaaggcgatgatggcgccgaccaatccgctcggcatcgtctcggcaacggtctacctgtggaagcgcgatccgaaaaccggcgagtggttcgaagtcgtcggccaggcctattgggatgagttcgcgccgatcaagcgcaaggccgccggcggatacaagtgggaggacactggcgaggtctggcccgatagcggcaagccgaaaaggaagaaggtggccgtcggcgaggaaacggaagtcctcgacgattccggcaactggtgccgcatgccacgcctgatgattgagaagtgtgctcagatgcaggcacttcgcgccggctggccggaagaatttaccggcacctatgacgaggccgaaatggaccgcgccaaggttctcgacctgacggcttccgagattgccgcccaggagcagcaggacaaccggctgcgcgcgatcggcggcaaggacgccctcaccatttggtggagcgaaggcttcggtctcgaaaacgttcctgatggaaagttcttcgatcgcgtcatcgaccatatcgccaatctcgatgcgaccgccgtggcgaagtgggaagacgcgaaccgcgctagcctgcaaatttattgggctcgtcatcccggcgacgccctcgaactgaagaagcgcatcgaggctgcacgccagaagcgcgcggcgaacgtcgacaaggcgctgcaacagcatccgttgatggcaggctga BBa_K2121007_sequence 1 ccttttgctggccttttgctcaagcttgcatgcctgcaggtcgagatccccagcccgcctaatgagcgggcttttttttggtcgacaccggtcacgtgcctaggactagtacatgcggcttcggaacataggcgtgcagcggcgtgtcgatcgaggcggcgcgggggttgaggttgaaagtgcaatggggcaggtagccgaccatctcgtcttcgaattcgacgccgcgatagatgcccttcagcgcgcggagcttgtccatgtagagggtggagaagccatccatcttgatcgtgccgacgtcgccgccggagcccttgacccagaggacctcgacaatttcgccggtcagcggatccttttccatgaccttggcggaggtattgccgccgccgtaattggtgatccgcttgtccgatccgaggaggttcgaacggtagaggagcttttccggctcggtcattcctgccgccttgccgtcgtcccaaagattggcaaggcgcgcgccctggtgcttgtcgagcatgtccaactcctcccgctgatctggctgccgcaacatgaaccgcggccatatgattggcaccagatcacacggtgattacgcggatgtcaatcaaaaacgatcaaaaacgttcatgatgcgccgcacaatggaaaaacatgattgattgtgattgacaatgctcggaatggatggaagcatgcggagcatcggaggagcgcatgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtacttccaatccatggagaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgatgtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaacggtctccagtaaaggtggataaagcttgatgggggatcccatggtacgcgtgctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggagcttagcttgagcttggatcagattgtcgtttcccgccttcagtttaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcgtttattagaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttcccctcgggatcaaagtactttaaagtactttaaagtactttaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgacctactggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaacgtctctttcctgtggatagcacgtacattgggaacccaaagccgtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcatgatatatctcccaatttgtgtagggcttattatgcacgcttaaaaataataaaagcagacttgacctgatagtttggctgtgagcaattatgtgcttagtgcatctaatcgcttgagttaacgccgcgccgcgaagcggcgtcggcttgaacgaatttctagctagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttcccccatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtaccccaaaaaaacatgtcataacaagaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcattacagcttacgaa BBa_K2121002_sequence 1 atgaacgcaatgacccgctatgacatgacgccgaagcagatttcgctcgtcaaaacgacgatcgcgaaggactgcaacgatgaagagtttaatctgttttgcgaagtcgcccgcgcgaagggacttgatccctttctcgggcagatcatcccgatggtcttttcgaagggcgatgccgaaaaacggaagatgactatcatcatcacccgcgacggccagcgcgtgattgctcaacgctgcggcgactatcggccggccagcaagccgacacagttcgaagtcgacaaggcgatgatggcgccgaccaatccgctcggcatcgtctcggcaacggtctacctgtggaagcgcgatccgaaaaccggcgagtggttcgaagtcgtcggccaggcctattgggatgagttcgcgccgatcaagcgcaaggccgccggcggatacaagtgggaggacactggcgaggtctggcccgatagcggcaagccgaaaaggaagaaggtggccgtcggcgaggaaacggaagtcctcgacgattccggcaactggtgccgcatgccacgcctgatgattgagaagtgtgctcagatgcaggcacttcgcgccggctggccggaagaatttaccggcacctatgacgaggccgaaatggaccgcgccaaggttctcgacctgacggcttccgagattgccgcccaggagcagcaggacaaccggctgcgcgcgatcggcggcaaggacgccctcaccatttggtggagcgaaggcttcggtctcgaaaacgttcctgatggaaagttcttcgatcgcgtcatcgaccatatcgccaatctcgatgcgaccgccgtggcgaagtgggaagacgcgaaccgcgctagcctgcaaatttattgggctcgtcatcccggcgacgccctcgaactgaagaagcgcatcgaggctgcacgccagaagcgcgcggcgaacgtcgacaaggcgctgcaacagcatccgttgatggcaggctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z