BBa_K2121003
1
BBa_K2121003
SPP1_GP35_Q38143
2016-10-19T11:00:00Z
2016-10-20T10:40:37Z
Sequence ID: X97918.2, SPP1_GP35_Q38143
Coding sequence for beta recombinase protein homolog.
For use in recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (ie S. meliloti and CIAT 899). Identified with BLASTp from homology to the SPP1 35 (Q38143) B. subtillus phage beta-like recombination protein. Derived from phage that selectively infects R.
Within Yale iGEM 2016, known as gBlock 14, amplified for cloning with primers priG 459, 460.
Uniprot SPP1_GP35_Q38143 . E-value 4.90E-12
false
false
_2590_
33231
33231
9
false
Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti.
false
Jinny van Doorn
annotation2525734
1
cds SPP1 page recombination protein
range2525734
1
1
864
BBa_K2121011
1
BBa_K2121011
Rhamnose_SPP1_GP35_Q38143
2016-10-20T11:00:00Z
2016-10-21T12:58:14Z
Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.
Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).
false
false
_2590_
30243
30243
9
false
Expression fusion with native S. meliloti protein.
false
Leslie Wang
component2527490
1
BBa_K2121003
component2527488
1
BBa_K2121007
annotation2527488
1
BBa_K2121007
range2527488
1
1
45
annotation2527490
1
BBa_K2121003
range2527490
1
52
915
BBa_K2121007
1
BBa_K2121007
Rhamnose Inducible Promoter
2016-10-20T11:00:00Z
2016-10-29T09:03:27Z
Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.
Promoter regions in S. meliloti inducible by rhamnose.
false
false
_2590_
30243
30243
9
false
Utilize native activation mechanism in S. meliloti.
false
Leslie Wang
annotation2531256
1
specR
range2531256
1
7074
8006
annotation2531250
1
T1 terminator
range2531250
1
1572
1640
annotation2531254
1
pVS1 repA
range2531254
1
4201
5308
annotation2531247
1
LIC Upstream site
range2531247
1
772
786
annotation2531253
1
ORF 2
range2531253
1
4081
4675
annotation2531252
1
pVS1 staA
range2531252
1
3177
3806
annotation2531249
1
LIC Downstream Site
range2531249
1
1521
1535
annotation2531245
1
short chain dehydrogenase
range2531245
1
111
526
annotation2531251
1
ORF 1
range2531251
1
2192
2878
annotation2531244
1
trp terminator
range2531244
1
52
79
annotation2531257
1
spect
range2531257
1
7074
8073
annotation2531255
1
pBR322 origin
range2531255
1
6223
6842
annotation2531246
1
SMc02323; PUTATIVE TRANSCRIPTION REGULATOR PROTEIN
range2531246
1
721
771
annotation2531248
1
citrine
range2531248
1
794
1512
BBa_K2121011_sequence
1
atgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtactagatggcaactaaaaaacaagaggaattaaaaaacgcactagcacaacaaaacggagccgttccgcaaacaccagtcaagccgcaggataaagtaaagggctatctcgaaagaatgatgccagctattaaagatgtgttgcctaagcatttagacgctgatcgactttcccgcatcgcaatgaacgtcattaggactaacccgaaacttctggaatgtgatacggcatcactcatgggggctgtgctggagtcagctaaattaggagtggagccgggacttttgggacaagcgtatattttgccgtacacgaattacaaaaagaaaacggttgaagctcagttcattcttggttataagggcctccttgatctcgtgagacgctctggacacgtgagcacaatcagcgctcaaactgtttacaaaaacgacacattcgaatatgaatatgggttagatgacaagcttgttcacagacccgcaccattcggaacagacagaggggagccagttggatactacgctgtagcgaaaatgaaggatggcggatataacttccttgtcatgagtaaacaggatgtcgagaaacaccgggacgccttttccaagtctaaaaacagagagggagtagtgtatggcccatgggctgaccacttcgacgcaatggcgaaaaagacagtcttgcgccagcttatcaactatttacctatcagtgttgagcaattatcaggagtagcggcagacgaacggacaggatcagaacttcataaccaatttgcggatgacgacaacattattaacgtcgatatcaatacaggtgaaatcattgaccatcaagaaaaattaggaggggaaacaaatgaatag
BBa_K2121003_sequence
1
atggcaactaaaaaacaagaggaattaaaaaacgcactagcacaacaaaacggagccgttccgcaaacaccagtcaagccgcaggataaagtaaagggctatctcgaaagaatgatgccagctattaaagatgtgttgcctaagcatttagacgctgatcgactttcccgcatcgcaatgaacgtcattaggactaacccgaaacttctggaatgtgatacggcatcactcatgggggctgtgctggagtcagctaaattaggagtggagccgggacttttgggacaagcgtatattttgccgtacacgaattacaaaaagaaaacggttgaagctcagttcattcttggttataagggcctccttgatctcgtgagacgctctggacacgtgagcacaatcagcgctcaaactgtttacaaaaacgacacattcgaatatgaatatgggttagatgacaagcttgttcacagacccgcaccattcggaacagacagaggggagccagttggatactacgctgtagcgaaaatgaaggatggcggatataacttccttgtcatgagtaaacaggatgtcgagaaacaccgggacgccttttccaagtctaaaaacagagagggagtagtgtatggcccatgggctgaccacttcgacgcaatggcgaaaaagacagtcttgcgccagcttatcaactatttacctatcagtgttgagcaattatcaggagtagcggcagacgaacggacaggatcagaacttcataaccaatttgcggatgacgacaacattattaacgtcgatatcaatacaggtgaaatcattgaccatcaagaaaaattaggaggggaaacaaatgaatag
BBa_K2121007_sequence
1
ccttttgctggccttttgctcaagcttgcatgcctgcaggtcgagatccccagcccgcctaatgagcgggcttttttttggtcgacaccggtcacgtgcctaggactagtacatgcggcttcggaacataggcgtgcagcggcgtgtcgatcgaggcggcgcgggggttgaggttgaaagtgcaatggggcaggtagccgaccatctcgtcttcgaattcgacgccgcgatagatgcccttcagcgcgcggagcttgtccatgtagagggtggagaagccatccatcttgatcgtgccgacgtcgccgccggagcccttgacccagaggacctcgacaatttcgccggtcagcggatccttttccatgaccttggcggaggtattgccgccgccgtaattggtgatccgcttgtccgatccgaggaggttcgaacggtagaggagcttttccggctcggtcattcctgccgccttgccgtcgtcccaaagattggcaaggcgcgcgccctggtgcttgtcgagcatgtccaactcctcccgctgatctggctgccgcaacatgaaccgcggccatatgattggcaccagatcacacggtgattacgcggatgtcaatcaaaaacgatcaaaaacgttcatgatgcgccgcacaatggaaaaacatgattgattgtgattgacaatgctcggaatggatggaagcatgcggagcatcggaggagcgcatgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtacttccaatccatggagaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgatgtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaacggtctccagtaaaggtggataaagcttgatgggggatcccatggtacgcgtgctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggagcttagcttgagcttggatcagattgtcgtttcccgccttcagtttaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcgtttattagaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttcccctcgggatcaaagtactttaaagtactttaaagtactttaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgacctactggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaacgtctctttcctgtggatagcacgtacattgggaacccaaagccgtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcatgatatatctcccaatttgtgtagggcttattatgcacgcttaaaaataataaaagcagacttgacctgatagtttggctgtgagcaattatgtgcttagtgcatctaatcgcttgagttaacgccgcgccgcgaagcggcgtcggcttgaacgaatttctagctagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttcccccatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtaccccaaaaaaacatgtcataacaagaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcattacagcttacgaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z