BBa_K2121011 1 BBa_K2121011 Rhamnose_SPP1_GP35_Q38143 2016-10-20T11:00:00Z 2016-10-21T12:58:14Z Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search. Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med). false false _2590_ 30243 30243 9 false Expression fusion with native S. meliloti protein. false Leslie Wang component2527490 1 BBa_K2121003 component2527488 1 BBa_K2121007 annotation2527488 1 BBa_K2121007 range2527488 1 1 45 annotation2527490 1 BBa_K2121003 range2527490 1 52 915 BBa_K2121003 1 BBa_K2121003 SPP1_GP35_Q38143 2016-10-19T11:00:00Z 2016-10-20T10:40:37Z Sequence ID: X97918.2, SPP1_GP35_Q38143 Coding sequence for beta recombinase protein homolog. For use in recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (ie S. meliloti and CIAT 899). Identified with BLASTp from homology to the SPP1 35 (Q38143) B. subtillus phage beta-like recombination protein. Derived from phage that selectively infects R. Within Yale iGEM 2016, known as gBlock 14, amplified for cloning with primers priG 459, 460. Uniprot SPP1_GP35_Q38143 . E-value 4.90E-12 false false _2590_ 33231 33231 9 false Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti. false Jinny van Doorn annotation2525734 1 cds SPP1 page recombination protein range2525734 1 1 864 BBa_K2121007 1 BBa_K2121007 Rhamnose Inducible Promoter 2016-10-20T11:00:00Z 2016-10-29T09:03:27Z Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1. Promoter regions in S. meliloti inducible by rhamnose. false false _2590_ 30243 30243 9 false Utilize native activation mechanism in S. meliloti. false Leslie Wang annotation2531244 1 trp terminator range2531244 1 52 79 annotation2531250 1 T1 terminator range2531250 1 1572 1640 annotation2531255 1 pBR322 origin range2531255 1 6223 6842 annotation2531249 1 LIC Downstream Site range2531249 1 1521 1535 annotation2531248 1 citrine range2531248 1 794 1512 annotation2531256 1 specR range2531256 1 7074 8006 annotation2531246 1 SMc02323; PUTATIVE TRANSCRIPTION REGULATOR PROTEIN range2531246 1 721 771 annotation2531251 1 ORF 1 range2531251 1 2192 2878 annotation2531245 1 short chain dehydrogenase range2531245 1 111 526 annotation2531257 1 spect range2531257 1 7074 8073 annotation2531252 1 pVS1 staA range2531252 1 3177 3806 annotation2531253 1 ORF 2 range2531253 1 4081 4675 annotation2531247 1 LIC Upstream site range2531247 1 772 786 annotation2531254 1 pVS1 repA range2531254 1 4201 5308 BBa_K2121011_sequence 1 atgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtactagatggcaactaaaaaacaagaggaattaaaaaacgcactagcacaacaaaacggagccgttccgcaaacaccagtcaagccgcaggataaagtaaagggctatctcgaaagaatgatgccagctattaaagatgtgttgcctaagcatttagacgctgatcgactttcccgcatcgcaatgaacgtcattaggactaacccgaaacttctggaatgtgatacggcatcactcatgggggctgtgctggagtcagctaaattaggagtggagccgggacttttgggacaagcgtatattttgccgtacacgaattacaaaaagaaaacggttgaagctcagttcattcttggttataagggcctccttgatctcgtgagacgctctggacacgtgagcacaatcagcgctcaaactgtttacaaaaacgacacattcgaatatgaatatgggttagatgacaagcttgttcacagacccgcaccattcggaacagacagaggggagccagttggatactacgctgtagcgaaaatgaaggatggcggatataacttccttgtcatgagtaaacaggatgtcgagaaacaccgggacgccttttccaagtctaaaaacagagagggagtagtgtatggcccatgggctgaccacttcgacgcaatggcgaaaaagacagtcttgcgccagcttatcaactatttacctatcagtgttgagcaattatcaggagtagcggcagacgaacggacaggatcagaacttcataaccaatttgcggatgacgacaacattattaacgtcgatatcaatacaggtgaaatcattgaccatcaagaaaaattaggaggggaaacaaatgaatag BBa_K2121003_sequence 1 atggcaactaaaaaacaagaggaattaaaaaacgcactagcacaacaaaacggagccgttccgcaaacaccagtcaagccgcaggataaagtaaagggctatctcgaaagaatgatgccagctattaaagatgtgttgcctaagcatttagacgctgatcgactttcccgcatcgcaatgaacgtcattaggactaacccgaaacttctggaatgtgatacggcatcactcatgggggctgtgctggagtcagctaaattaggagtggagccgggacttttgggacaagcgtatattttgccgtacacgaattacaaaaagaaaacggttgaagctcagttcattcttggttataagggcctccttgatctcgtgagacgctctggacacgtgagcacaatcagcgctcaaactgtttacaaaaacgacacattcgaatatgaatatgggttagatgacaagcttgttcacagacccgcaccattcggaacagacagaggggagccagttggatactacgctgtagcgaaaatgaaggatggcggatataacttccttgtcatgagtaaacaggatgtcgagaaacaccgggacgccttttccaagtctaaaaacagagagggagtagtgtatggcccatgggctgaccacttcgacgcaatggcgaaaaagacagtcttgcgccagcttatcaactatttacctatcagtgttgagcaattatcaggagtagcggcagacgaacggacaggatcagaacttcataaccaatttgcggatgacgacaacattattaacgtcgatatcaatacaggtgaaatcattgaccatcaagaaaaattaggaggggaaacaaatgaatag BBa_K2121007_sequence 1 ccttttgctggccttttgctcaagcttgcatgcctgcaggtcgagatccccagcccgcctaatgagcgggcttttttttggtcgacaccggtcacgtgcctaggactagtacatgcggcttcggaacataggcgtgcagcggcgtgtcgatcgaggcggcgcgggggttgaggttgaaagtgcaatggggcaggtagccgaccatctcgtcttcgaattcgacgccgcgatagatgcccttcagcgcgcggagcttgtccatgtagagggtggagaagccatccatcttgatcgtgccgacgtcgccgccggagcccttgacccagaggacctcgacaatttcgccggtcagcggatccttttccatgaccttggcggaggtattgccgccgccgtaattggtgatccgcttgtccgatccgaggaggttcgaacggtagaggagcttttccggctcggtcattcctgccgccttgccgtcgtcccaaagattggcaaggcgcgcgccctggtgcttgtcgagcatgtccaactcctcccgctgatctggctgccgcaacatgaaccgcggccatatgattggcaccagatcacacggtgattacgcggatgtcaatcaaaaacgatcaaaaacgttcatgatgcgccgcacaatggaaaaacatgattgattgtgattgacaatgctcggaatggatggaagcatgcggagcatcggaggagcgcatgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtacttccaatccatggagaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgatgtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaacggtctccagtaaaggtggataaagcttgatgggggatcccatggtacgcgtgctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggagcttagcttgagcttggatcagattgtcgtttcccgccttcagtttaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcgtttattagaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttcccctcgggatcaaagtactttaaagtactttaaagtactttaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgacctactggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaacgtctctttcctgtggatagcacgtacattgggaacccaaagccgtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcatgatatatctcccaatttgtgtagggcttattatgcacgcttaaaaataataaaagcagacttgacctgatagtttggctgtgagcaattatgtgcttagtgcatctaatcgcttgagttaacgccgcgccgcgaagcggcgtcggcttgaacgaatttctagctagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttcccccatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtaccccaaaaaaacatgtcataacaagaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcattacagcttacgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z