BBa_K2121004
1
BBa_K2121004
Lambda_Beta_F7X3D1 (red_rhizo-MAGE)
2016-10-19T11:00:00Z
2016-10-20T10:47:07Z
Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11). Gene: bet, Ordered Locus Names:SM11_chr1058
Coding sequence for beta recombinase protein homolog.
For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (S. meliloti and CIAT 899). Identified with BLASTp from homology to the lambda coliphage beta recombinase. Derived from phage that selectively infects rhizobia spp (Sinorhizobium meliloti strain SM11)
Within Yale iGEM 2016, known as gBlock 15, amplified for cloning with primers priG 461, 462
Unitprot KB F7X3D1_SINMM, E value 1.50E-36
false
false
_2590_
33231
33231
9
false
Elimination of any illegal restriction sites, and codon optimization for expression.
false
Jinny van Doorn
annotation2525738
1
cds recombination protein bet F7X3D1
range2525738
1
1
951
BBa_K2121007
1
BBa_K2121007
Rhamnose Inducible Promoter
2016-10-20T11:00:00Z
2016-10-29T09:03:27Z
Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.
Promoter regions in S. meliloti inducible by rhamnose.
false
false
_2590_
30243
30243
9
false
Utilize native activation mechanism in S. meliloti.
false
Leslie Wang
annotation2531256
1
specR
range2531256
1
7074
8006
annotation2531250
1
T1 terminator
range2531250
1
1572
1640
annotation2531254
1
pVS1 repA
range2531254
1
4201
5308
annotation2531247
1
LIC Upstream site
range2531247
1
772
786
annotation2531253
1
ORF 2
range2531253
1
4081
4675
annotation2531252
1
pVS1 staA
range2531252
1
3177
3806
annotation2531249
1
LIC Downstream Site
range2531249
1
1521
1535
annotation2531245
1
short chain dehydrogenase
range2531245
1
111
526
annotation2531251
1
ORF 1
range2531251
1
2192
2878
annotation2531244
1
trp terminator
range2531244
1
52
79
annotation2531257
1
spect
range2531257
1
7074
8073
annotation2531255
1
pBR322 origin
range2531255
1
6223
6842
annotation2531246
1
SMc02323; PUTATIVE TRANSCRIPTION REGULATOR PROTEIN
range2531246
1
721
771
annotation2531248
1
citrine
range2531248
1
794
1512
BBa_K2121012
1
BBa_K2121012
Rhamnose_Lambda_Beta_F7X3D1
2016-10-20T11:00:00Z
2016-10-21T12:59:37Z
Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.
Expression cassette with rhamnose inducible promoter and coding sequence for beta recombinase protein homolog. For use in red recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species. Identified from homology to the lambda coliphage beta recombinase via BLASTp search. Derived from phage that selectively infects rhizobia spp (S. med).
false
false
_2590_
30243
30243
9
false
Expression fusion with native S. meliloti protein.
false
Leslie Wang
component2527493
1
BBa_K2121004
component2527491
1
BBa_K2121007
annotation2527493
1
BBa_K2121004
range2527493
1
52
1002
annotation2527491
1
BBa_K2121007
range2527491
1
1
45
BBa_K2121012_sequence
1
atgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtactagatgaaccagcttgcaaaagcgccgcgccggtctgtgcttgtcgatatggccgcgcacttcggcatggaggccgatgccttcgaaatgacggtgcgggcgcaatgctcgccgaccccaaagaagggcgagcaattccgtccgctgacccgcgaggagtttgccgccttcctgctggtcgccaagaaatacgatctcaatccgctaacccgcgagattttcgcctatccgaagcgcggcggcggcgtcgtgccgatcgtctcgattgacgggtggatcaacctcgtcaattcgcacccggcttgcgacggctttgagttcacgtgggagcgcgatgccaacggcgatccgatttcttgcacatgcatcatgcatcgcaaggaccgaagccacccgaccgccgtcaccgaatacctcgccgagtgctggcgcgacaccgaaccgtggaagatgaagcaccgcatgttgcgccacaaggcgctgatgcaatgcgcccgctatgccttcggctttgccggcatctacgacgaggacgagggccggcggatcgccgaagatcagaacattgccctattgccgccagcgccgcgcgcgccgcgcataggtcagcagagccctgctggtgaaaaaatccagacggcgcagggcgatgcgacggaggtcgagtccgggaccgggcagccgccggtagactcggcctccccccttgatgacgagcccgaccccgatcgcgaggaaataggcggcgtcgatgccgactccaccccggacgcggagttctttgacgaactgcgcgaccggctggccgaggcgaaagacgccgccagcgtggaggaaatctggacagagcttgacccgatggcgcggttcgaaggctccgaccttgatcaagagatttgccagaagatcaaagcgcgccggctgcgcgatctggaaaaggaggatgggaaatga
BBa_K2121007_sequence
1
ccttttgctggccttttgctcaagcttgcatgcctgcaggtcgagatccccagcccgcctaatgagcgggcttttttttggtcgacaccggtcacgtgcctaggactagtacatgcggcttcggaacataggcgtgcagcggcgtgtcgatcgaggcggcgcgggggttgaggttgaaagtgcaatggggcaggtagccgaccatctcgtcttcgaattcgacgccgcgatagatgcccttcagcgcgcggagcttgtccatgtagagggtggagaagccatccatcttgatcgtgccgacgtcgccgccggagcccttgacccagaggacctcgacaatttcgccggtcagcggatccttttccatgaccttggcggaggtattgccgccgccgtaattggtgatccgcttgtccgatccgaggaggttcgaacggtagaggagcttttccggctcggtcattcctgccgccttgccgtcgtcccaaagattggcaaggcgcgcgccctggtgcttgtcgagcatgtccaactcctcccgctgatctggctgccgcaacatgaaccgcggccatatgattggcaccagatcacacggtgattacgcggatgtcaatcaaaaacgatcaaaaacgttcatgatgcgccgcacaatggaaaaacatgattgattgtgattgacaatgctcggaatggatggaagcatgcggagcatcggaggagcgcatgcacgagaaagaaagacacaggatcatcctgtcggcggtgcagtacttccaatccatggagaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgatgtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaacggtctccagtaaaggtggataaagcttgatgggggatcccatggtacgcgtgctagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagacctaggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggagcttagcttgagcttggatcagattgtcgtttcccgccttcagtttaaactatcagtgtttgacaggatatattggcgggtaaacctaagagaaaagagcgtttattagaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccatttgtatgtgcatgccaaccacagggttcccctcgggatcaaagtactttaaagtactttaaagtactttaaagtactttgatccaacccctccgctgctatagtgcagtcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaaccggagacattacgccatgaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccgacgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgcaccaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcgacctactggacattgccgagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcgggcgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatcgcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgatccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgcagccaagcccttacgacatatgggccaccgccgacctggtggagctggttaagcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgctggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgttcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcgggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaagaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccggccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggtcgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccagacgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtgggattacgacctggtactgatggcggtttcccatctaaccgaatccatgaaccgataccgggaagggaagggagacaagcccggccgcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccgagggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttcaccccgattactttttgatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctagggcaaattgccctagcaggggaaaaaggtcgaaaacgtctctttcctgtggatagcacgtacattgggaacccaaagccgtacattgggaaccggaacccgtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaagtgactgatataaaagagaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgcatgatatatctcccaatttgtgtagggcttattatgcacgcttaaaaataataaaagcagacttgacctgatagtttggctgtgagcaattatgtgcttagtgcatctaatcgcttgagttaacgccgcgccgcgaagcggcgtcggcttgaacgaatttctagctagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttcccccatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtaccccaaaaaaacatgtcataacaagaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcattacagcttacgaa
BBa_K2121004_sequence
1
atgaaccagcttgcaaaagcgccgcgccggtctgtgcttgtcgatatggccgcgcacttcggcatggaggccgatgccttcgaaatgacggtgcgggcgcaatgctcgccgaccccaaagaagggcgagcaattccgtccgctgacccgcgaggagtttgccgccttcctgctggtcgccaagaaatacgatctcaatccgctaacccgcgagattttcgcctatccgaagcgcggcggcggcgtcgtgccgatcgtctcgattgacgggtggatcaacctcgtcaattcgcacccggcttgcgacggctttgagttcacgtgggagcgcgatgccaacggcgatccgatttcttgcacatgcatcatgcatcgcaaggaccgaagccacccgaccgccgtcaccgaatacctcgccgagtgctggcgcgacaccgaaccgtggaagatgaagcaccgcatgttgcgccacaaggcgctgatgcaatgcgcccgctatgccttcggctttgccggcatctacgacgaggacgagggccggcggatcgccgaagatcagaacattgccctattgccgccagcgccgcgcgcgccgcgcataggtcagcagagccctgctggtgaaaaaatccagacggcgcagggcgatgcgacggaggtcgagtccgggaccgggcagccgccggtagactcggcctccccccttgatgacgagcccgaccccgatcgcgaggaaataggcggcgtcgatgccgactccaccccggacgcggagttctttgacgaactgcgcgaccggctggccgaggcgaaagacgccgccagcgtggaggaaatctggacagagcttgacccgatggcgcggttcgaaggctccgaccttgatcaagagatttgccagaagatcaaagcgcgccggctgcgcgatctggaaaaggaggatgggaaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z