LOCUS       BBa_K2121013            2126 bp    DNA     linear       20-Sep-2024
DEFINITION  AStr_Citrine_T1
ACCESSION   BBa_K2121013
VERSION     BBa_K2121013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=promoter pJ23100
     RBS             36..39
                     /label=RBS
     misc_feature    40..54
                     /label=Upstream LIC cloning site
     CDS             470..1841
                     /label=citrine ORF
     misc_feature    1880..1916
                     /label=terT1_LIC-R
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcaggat acttccaatc catggagacc
       61 gacgtccaca tatacctgcc gttcactatt atttagtgaa atgagatatt atgatatttt
      121 ctgaattgtg attaaaaagg caactttatg cccatgcaac agaaactata aaaaatacag
      181 agaatgaaaa gaaacagata gattttttag ttctttaggc ccgtagtctg caaatccttt
      241 tatgattttc tatcaaacaa atccaaacga gagtctaata gaatgagggt ttgttactga
      301 taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt
      361 acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc
      421 tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa
      481 aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc
      541 aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc
      601 ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatataa
      661 agttcctgag ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt
      721 ttgggacagc tggccattac aaaacactga cggcactgtc gcaaactatc acggctacca
      781 catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt
      841 ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa
      901 agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc
      961 aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg
     1021 taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag
     1081 ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac
     1141 gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac
     1201 gctgagagat cctcactacg tagaagataa agaaacactg gaactgaaga tggctaaaag
     1261 catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg
     1321 ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg
     1381 attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa
     1441 ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg
     1501 gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt
     1561 ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg
     1621 atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag
     1681 gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat
     1741 caacgtttgc gcctagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag
     1801 acagcatcct tgaacaagga caattaacag ttaacaaata aaaacgcaaa agaaaatgcc
     1861 gatatcctat tggcattgac ggtctccagt aaaggtggat aaagcttgat gggggatccc
     1921 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1981 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     2041 gacctaggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2101 tccacagaat caggggataa cgcagg
//