LOCUS BBa_K2122000 2401 bp DNA linear 22-Nov-2024 DEFINITION Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter ACCESSION BBa_K2122000 VERSION BBa_K2122000.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2298 /label=BBa_K2122150 stem_loop 2314..2333 /label=T7 TE misc_feature 2340..2340 /label=stop polyA_site 2334..2347 /label=polya misc_feature 2307..2347 /label=BBa_B0012 terminator 2307..2347 /label=BBa_B0012 stem_loop 2368..2390 /label=stem_loop misc_feature 2356..2401 /label=BBa_B0011 terminator 2356..2401 /label=BBa_B0011 terminator 2307..2401 /label=BBa_B0014 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccaagccccc cgacctcctg 1261 ctgcggctgc tccggggcgc cccaaggcag cgggtctgca ccctgttcat catcggcttc 1321 aagttcacgt ttttcgtctc catcatgatc tactggcacg ttgtgggaga gcccaaggag 1381 aaagggcagc tctataacct gccagcagag atcccctgcc ccaccttgac accccccacc 1441 ccaccctccc acggccccac tccaggcaac atcttcttcc tggagacttc agaccggacc 1501 aaccccaact tcctgttcat gtgctcggtg gagtcggccg ccagaactca ccccgaatcc 1561 cacgtgctgg tcctgatgaa agggcttccg ggtggcaacg cctctctgcc ccggcacctg 1621 ggcatctcac ttctgagctg cttcccgaat gtccagatgc tcccgctgga cctgcgggag 1681 ctgttccggg acacacccct ggccgactgg tacgcggccg tgcaggggcg ctgggagccc 1741 tacctgctgc ccgtgctctc cgacgcctcc aggatcgcac tcatgtggaa gttcggcggc 1801 atctacctgg acacggactt cattgttctc aagaacctgc ggaacctgac caacgtgctg 1861 ggcacccagt cccgctacgt cctcaacggc gcgttcctgg ccttcgagcg ccggcacgag 1921 ttcatggcgc tgtgcatgcg ggacttcgtg gaccactaca acggctggat ctggggtcac 1981 cagggcccgc agctgctcac gcgggtcttc aagaagtggt gttccatccg cagcctggcc 2041 gagagccgcg cctgccgcgg cgtcaccacc ctgccccctg aggccttcta ccccatcccc 2101 tggcaggact ggaagaagta ctttgaggac atcaaccccg aggagctgcc gcggctgctc 2161 agtgccacct atgctgtcca cgtgtggaac aagaagagcc agggcacgcg gttcgaggcc 2221 acgtccaggg cactgctggc ccagctgcat gcccgctact gccccacgac gcacgaggcc 2281 atgaaaatgt acttgtgata ctagagtcac actggctcac cttcgggtgg gcctttctgc 2341 gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt 2401 t //