LOCUS       BBa_K2122200            2401 bp    DNA     linear       25-Nov-2024
DEFINITION  His-tagged Shiga-Like Subunit B toxin
ACCESSION   BBa_K2122200
VERSION     BBa_K2122200.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..2298
                     /label=BBa_K2122151
     stem_loop       2314..2333
                     /label=T7 TE
     misc_feature    2340..2340
                     /label=stop
     polyA_site      2334..2347
                     /label=polya
     misc_feature    2307..2347
                     /label=BBa_B0012
     terminator      2307..2347
                     /label=BBa_B0012
     stem_loop       2368..2390
                     /label=stem_loop
     misc_feature    2356..2401
                     /label=BBa_B0011
     terminator      2356..2401
                     /label=BBa_B0011
     terminator      2307..2401
                     /label=BBa_B0014
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccaagccccc cgacctcctg
     1261 ctgcggctgc tccggggcgc cccaaggcag cgggtctgca ccctgttcat catcggcttc
     1321 aagttcacgt ttttcgtctc catcatgatc tactggcacg ttgtgggaga gcccaaggag
     1381 aaagggcagc tctataacct gccagcagag atcccctgcc ccaccttgac accccccacc
     1441 ccaccctccc acggccccac tccaggcaac atcttcttcc tggagacttc agaccggacc
     1501 aaccccaact tcctgttcat gtgctcggtg gagtcggccg ccagaactca ccccgaatcc
     1561 cacgtgctgg tcctgatgaa agggcttccg ggtggcaacg cctctctgcc ccggcacctg
     1621 ggcatctcac ttctgagctg cttcccgaat gtccagatgc tcccgctgga cctgcgggag
     1681 ctgttccggg acacacccct ggccgactgg tacgcggccg tgcaggggcg ctgggagccc
     1741 tacctgctgc ccgtgctctc cgacgcctcc aggatcgcac tcatgtggaa gttcggcggc
     1801 atctacctgg acacggactt cattgttctc aagaacctgc ggaacctgac caacgtgctg
     1861 ggcacccagt cccgctacgt cctcaacggc gcgttcctgg ccttcgagcg ccggcacgag
     1921 ttcatggcgc tgtgcatgcg ggacttcgtg gaccactaca acggctggat ctggggtcac
     1981 cagggcccgc agctgctcac gcgggtcttc aagaagtggt gttccatccg cagcctggcc
     2041 gagagccgcg cctgccgcgg cgtcaccacc ctgccccctg aggccttcta ccccatcccc
     2101 tggcaggact ggaagaagta ctttgaggac atcaaccccg aggagctgcc gcggctgctc
     2161 agtgccacct atgctgtcca cgtgtggaac aagaagagcc agggcacgcg gttcgaggcc
     2221 acgtccaggg cactgctggc ccagctgcat gcccgctact gccccacgac gcacgaggcc
     2281 atgaaaatgt acttgtgata ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2341 gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt
     2401 t
//