LOCUS BBa_K2123204 3480 bp DNA linear 29-Nov-2024 DEFINITION Bioaccumulator device: Strong promoter + OmpA fused to Synthetic Phytochelatin + B0015 ACCESSION BBa_K2123204 VERSION BBa_K2123204.1 FEATURES Location/Qualifiers RBS 3..10 /label=RBS phage T7 gene 10 CDS 19..451 /label=MerR (regulatory protein) coding sequence stem_loop 455..490 /label=Tryptophan terminator misc_feature 19..22 /label=Start codon misc_feature 449..451 /label=Stop codon misc_feature 1..497 /label=BBa_K2123200 misc_feature 506..548 /label=Trp Terminator CDS 549..985 /label=MerR promoter 991..1019 /label=Bidirecional promoter promoter 1015..1043 /label=Bidirecional promoter CDS 1056..1405 /label=MerT RBS 1406..1412 /label=RBS CDS 1419..1694 /label=MerP RBS 995..1001 /label=RBS RBS 1042..1048 /label=RBS misc_feature 506..1694 /label=BBa_K1355001 misc_feature 506..1694 /label=BBa_K2123202 RBS 1711..1718 /label=rbs misc_feature 3354..3480 /label=BBa_ B0015 CDS 1726..3341 /label=MerA misc_feature 1703..3480 /label=BBa_K1355000 ORIGIN 1 ctagaaggag tcaagcgata tggaaaataa tttggaaaac ctgaccattg gcgtttttgc 61 caaggcggcc ggggtcaacg tggagacaat ccgcttctat cagcgcaagg gcctgttgcg 121 ggaaccggac aagccttacg gcagcatccg ccgctatggg gaggcggacg tggttcgggt 181 gaaattcgtg aaatcggcac agcggctggg gttcagtctg gacgagattg ccgagctgtt 241 gcggctcgac gatggcaccc actgcgagga ggccagcagc ctggccgaac acaagctcaa 301 ggacgtgcgc gagaagatgg ccgacttggc gcgcatggaa accgtgctgt ctgaactcgt 361 gtgcgcctgc catgcacgaa aggggaatgt ttcctgcccg ttgatcgcgt cactacaggg 421 cgaagcaggc ctggcaaggt cagctatgcc ttagtcccac agccgccagt tccgctggcg 481 gcattttaac tttcttttac tagagaaaga aagttaaaat gccgccagcg gaactggcgg 541 ctgtgggact aaggcatagc tgaccttgcc aggcctgctt cgccctgtag tgacgcgatc 601 aacgggcagg aaacattccc ctttcgtgca tggcaggcgc acacgagttc agacagcacg 661 gtttccatgc gcgccaagtc ggccatcttc tcgcgcacgt ccttgagctt gtgttcggcc 721 aggctgctgg cctcctcgca gtgggtgcca tcgtcgagcc gcaacagctc ggcaatctcg 781 tccagactga accccagccg ctgtgccgat ttcacgaatt tcacccgaac cacgtccgcc 841 tccccatagc ggcggatgct gccgtaaggc ttgtccggtt cccgcaacag gcccttgcgc 901 tgatagaagc ggattgtctc cacgttgacc ccggccgcct tggcaaaaac gccaatggtc 961 aggttttcca aattattttc catatcgctt gactccgtac atgagtacgg aagtaaggtt 1021 acgctatcca atccaaattc aaaagggcca acgtatgtct gaaccacaaa acgggcgcgg 1081 tgcgctcttc gccggcgggc tggccgccat tcttgcatcg acctgctgcc tggggccgct 1141 agtactggtc gccctgggct tctccggtgc ttggatcggc aacctgacgg tgctggaacc 1201 ctatcgaccg ttgttcatcg gcgcggcgct agtggcgctg ttcttcgcct ggaagcggat 1261 ttaccggccc gtgcaggcat gcaagccagg tgaggtctgc gcgattccgc aggtgcgcgc 1321 cacctacaag ctgattttct ggatcgtggc cgtgctggtc ctggtcgcgc ttggatttcc 1381 ctatgtcgtt ccatttttct attaaccagg agttcatcat gaagaaactg tttgcctccc 1441 ttgccctcgc cgccgctgtt gccccggtgt gggccgctac ccagaccgtc acgctagcgg 1501 ttcccggcat gacttgcgcc gcctgcccga tcacagtcaa gaaagcgctc tccaaggtcg 1561 aaggcgtgag caaggtcgat gtgggcttcg agaagcgcga ggccgtcgtc acttttgacg 1621 acaccaaggc cagcgtacag aagctgacca aggccaccgc agacgccggc tatccgtcca 1681 gcgtcaagca gtgatactag agaactttaa gaaggagata tatccatgca atcagcggat 1741 gtctcctacg ccaagggcag cgccaagctc gccattgagg tcggcacgtc acccgacgcg 1801 ctgacggccg ctgtagctgg actcggttat cgggccacgc tggccgatgc cccctcagtt 1861 tcgacgccgg gcggattgct cgacaagatg cgcgatctgc tgggcagaaa cgacaagacg 1921 ggtagcagcg gcgcattgca tatcgccgtc atcggcagcg gcggggccgc gatggcagcg 1981 gcgctgaagg ccgtcgagca aggcgcacgt gtcacgctga tcgagcgcgg caccatcggc 2041 ggcacctgcg tcaatgtcgg ttgtgtgccg tccaagatca tgatccgcgc cgcccatatc 2101 gcccatctgc gccgggaaag cccgttcgat ggcggcatcg ccgctaccac gccgaccatc 2161 cagcgcacgg cgctgctggc ccagcagcag gcccgcgtcg atgaactgcg ccacgccaag 2221 tacgaaggca tcttggaggg caatccggcg atcactgtgc tgcacggctc cgcccgcttt 2281 aaggacaatc gcaacctgat cgtgcaactc aacgacggcg gcgagcgcgt ggtggcattc 2341 gaccgctgcc tgatcgccac cggcgcgagc ccggccgtgc cgccgattcc cggcctgaaa 2401 gacactccgt actggacttc cactgaagcg ctggtcagcg agacgattcc taagcgcctg 2461 gccgtgattg gctcatcagt ggtggcgctg gagctggcgc aggcgttcgc ccgactcgga 2521 gcgaaggtga cgatcctggc tcgcagcacg ctgttcttcc gcgaagaccc agctataggc 2581 gaagccgtca cggccgcatt ccgcatggag ggcatcgagg tgagggaaca cacccaggcc 2641 agccaggtcg cgtatatcaa tggtgaaggg gacggcgagt tcgtgctcac cacggcgcac 2701 ggcgaactgc gcgccgacaa gctgctggtc gccaccggcc gcgcgcccaa cacacgcaag 2761 ctggcactgg atgcgacggg cgtcacgctc accccgcaag gcgctatcgt catcgacccc 2821 ggcatgcgta caagcgtgga acacatctac gccgcaggcg actgcaccga ccagccgcag 2881 ttcgtctatg tggcggcagc ggccggcact cgcgccgcga tcaacatgac cggcggtgac 2941 gcggccctga acctgaccgc gatgccggcc gtggtgttca ccgacccgca agtggcgacc 3001 gtaggctaca gcgaggcgga agcgcaccat gacggcatca aaactgatag tcgcacgcta 3061 acgctggaca acgtgccgcg cgcgctcgcc aacttcgaca cgcgcggctt catcaaactg 3121 gtggttgaag aaggcagcgg acgactgatc ggcgtgcagg cagtggcccc ggaagcgggc 3181 gaactgatcc agacggccgc actggcgatt cgcaaccgga tgacggtgca ggaactggcc 3241 gaccagttgt tcccctacct gacgatggtc gaagggttga agctcgcggc gcagaccttc 3301 aacaaggatg tgaagcagct ttcctgctgc gccgggtgag gactactaga gccaggcatc 3361 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3421 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //