LOCUS       BBa_K2123301             774 bp    DNA     linear       22-Nov-2024
DEFINITION  Novel Synthetic Phytochelatin codon optimized for E. coli 
            expression
ACCESSION   BBa_K2123301
VERSION     BBa_K2123301.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..696
                     /label=mrfp1
     misc_feature    703..727
                     /label=Help:Barcodes
     CDS             22..727
                     /label=BBa_E1010
     misc_feature    736..774
                     /label=B1006
     stem_loop       745..766
                     /label=modified thr terminator
     polyA_site      767..774
                     /label=PolyA
     polyA_site      736..744
                     /label=PolyA
     terminator      736..774
                     /label=BBa_B1006
     misc_feature    1..774
                     /label=BBa_K081014
ORIGIN
        1 attaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
       61 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      121 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      181 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      241 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      301 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      361 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      421 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      481 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      541 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      601 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      661 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      721 agatcgctac tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt tttt
//