BBa_K2124003 1 BBa_K2124003 Venom Serine Protease from Cerastes Cerastes - Thrombin analog - DsbC Disulfide shuffling 2016-10-12T11:00:00Z 2016-10-21T02:01:09Z Both the DsbC and Venom Serine Protease are from genomic sequence. This Serine protease is a component of Cerastes Cerastes (Desert Horned Viper) venom. It recognizes Thrombin cut sites in fibrinogen and upon cleavage converts the fibrinogen to fibrin monomers. The fibrin monomers can then link and begin clot formation in mammals. This part is polycistronic and also contains DsbC which is secreted to the periplasmic space. DsbC shuffles the Serine Protease disulfide bonds for proper folding and protein activity. false false _2593_ 28641 28641 9 false This Serine Protease protein is very small and stabilized by several disulfide bonds. The final construct is secreted into the periplasmic space with DsbC, a protein that shuffles disfulfide bonds. DsbC will allow the Serine Protease to fold properly with correct disulfide bonding. This will give stable functional protein. The DsbC gene is contained as part of this construct. false Brian Dempsey BBa_K2124003_sequence 1 tttacggctagctcagccctaggtattatgctagctactagagattaaagaggagaaatactagagatgaaatacctgttgccgaccgcagccgccgggcttttgttgttggctgcacaaccggccatggccgtaatcggcggggctgaatgtaacatcaacgaacatcgctctttagtcttgctttacaattcctcccgcttgtttggaggaggaaccttgattaataaagagtgggtattgtcagcagcgcactgtgacggcgagaatatgaaaatttacctgggactgcatcatttccgtttacccaacaaggatcgccagattcgtgtggcgaaggaaaagtatttttgtcgtgatcgcaaatctattgttgacaaagatatcatgttaatcaagctgaacaaaccagttaacaattctacacacattgctccgttaagtcttccctcaagtccaccctccgtcggaagtgactgccgtatcatgggatggggcaccatcaccagtccaaatgatacgtatcctaaagtccctcattgcgctaacatcaacatcttagagcatagtttgtgtgagcgcgcgtataacgatttgtctgcgtcctcacgcaccttgtgcgcgggaatcgagaaaggtggtatcgacacctgtaagggagacagcggcggaccgttgatttgtaacggccagatccaaggaattgtttcatggggtgacgaggtttgcggaaaacctaataagcctggtgtatatactaaagtattcgactacacggactggattcgtaacattatcgccggaaacactgccgcgacttgcccgcagggaccgcgcccatagtagagattaaagaggagaaatactagagatgaaaaaaggttttatgctgtttacattattggcagcattctccggatttgcacaagcggatgacgctgcgattcagcagactttggccaagatgggtatcaaaagctcggacattcagcctgcaccggtagcgggaatgaagactgtgttgacaaactcgggagtgttatacattacagacgatgggaagcacattattcaaggacccatgtacgacgtttctggaacggctcccgtaaacgtgaccaacaagatgttactgaaacaattgaacgcactggaaaaggagatgattgtgtataaagccccccaagagaaacacgttattacagttttcaccgacatcacgtgcggctactgtcataaattgcatgagcagatggctgattacaatgcacttggaattacagtgcgttacttagccttcccgcgccagggattggattctgatgccgaaaaagaaatgaaggcaatttggtgtgctaaggataaaaacaaggcttttgatgacgtgatggcaggtaaatcggttgcacctgccagttgcgatgtcgatattgccgaccactatgcgcttggggtacaattgggtgtatccggcactccggcggtagtgttgtccaatgggacattagtgcccggttatcaaccaccaaaggagatgaaagagttccttgatgagcaccaaaagatgacgtctggcaagtagtagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z