LOCUS       BBa_K2127001            2071 bp    DNA     linear       22-Nov-2024
DEFINITION  Inducible High Expression His-Tagged Photosystem II CP47 subunit
ACCESSION   BBa_K2127001
VERSION     BBa_K2127001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..185
                     /label=Transcription Factor Binding Sites
     promoter        1..185
                     /label=BBa_K2127006
     CDS             186..273
                     /label=end of LacI coding region (inactive)
     misc_feature    186..385
                     /label=BBa_R0010
     misc_feature    274..311
                     /label=CAP binding site
     promoter        322..327
                     /label=-35
     promoter        346..351
                     /label=-10
     misc_feature    351..385
                     /label=LacI binding site
     misc_feature    358..358
                     /label=start
     promoter        186..385
                     /label=BBa_R0010
     RBS             386..400
                     /label=RBS-1\Strong
     RBS             393..397
                     /label=RBS
     misc_feature    386..400
                     /label=BBa_B0030
     RBS             386..400
                     /label=BBa_B0030
     misc_feature    401..403
                     /label=start
     CDS             401..1948
                     /label=CP47His
     misc_feature    1922..1939
                     /label=HisTag
     misc_feature    1943..1948
                     /label=double stop codon
     CDS             401..1948
                     /label=BBa_K2127004
     terminator      1949..2071
                     /label=BBa_K2127007
ORIGIN
        1 cctgtagaga agagtccctg aatatcaaaa tggtgggata aaaagctcaa aaaggaaagt
       61 aggctgtggt tccctaggca acagtcttcc ctaccccact ggaaactaaa aaaacgagaa
      121 aagttcgcac cgaacatcaa ttgcataatt ttagccctaa aacataagct gaacgaaact
      181 ggttgcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      241 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      301 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      361 tgtgagcgga taacaatttc acacaattaa agaggagaaa atgggactac cttggtatcg
      421 cgttcataca gttgtcctga atgatccagg gcgactcatc tctgtccatt taatgcacac
      481 tgccctagtg gctggctggg ctggatctat ggctctctat gagttggcca tttttgactc
      541 cagcgatgct gtgcttaacc ccatgtggcg gcaaggcatg tttgttttgc ccttcatggc
      601 ccgcctcggt gtcaccagtt cctggaatgg ctggagcgtc accggagaaa ctggtttgga
      661 tcccggtttc tggtcctttg aaggggtagc tgctgcccac atcgttctat ctggtctgtt
      721 gttcctagcc gccgtatggc actgggtatt ttgggacctg gaattatttg ttgacccccg
      781 taccggtgaa tccgcattgg atttgcccaa aatgtttggt attcacctct tcttgtctgg
      841 tttgctctgc ttcggttttg gtgctttcca cctcaccggc gtttggggcc ctggcatgtg
      901 ggtatccgac ccctatggtc tgacgggcca cgtacagcca gtggcaccgg aatggggacc
      961 agctggcttt aacccgttta accccggtgg ggtagtggct caccacattg ctgctggtat
     1021 tgtcggtatc attgccggtc ttttccacct tacggtacgg ccccctgagc gcttgtataa
     1081 agccctccgc atggggaata ttgagaccgt attgtccagt agtattgccg ctgtattttt
     1141 tgccgctttc gtcgtggccg gtaccatgtg gtacggtaat gccaccaccc ccatcgaact
     1201 ctttggcccc acccgttatc agtgggataa gggttatttt caagaagaaa tccaacgccg
     1261 ggttgatagc cagttagcag aaggagcttc cctatctgaa gcctggtcca ccatccctga
     1321 aaagttggct ttctacgatt acgtcggtaa cagccccgct aaaggtggtt tgtttcgtac
     1381 cggtgctatg aacagtggtg atggcattgc ccaggaatgg attggtcacc ccatattcaa
     1441 agacaaagaa ggtcgggaac tggaggtacg gcgtatgcct aacttctttg aaactttccc
     1501 cgtcatcatg accgatgcgg atggtgtagt ccgggcggat attcccttcc gtcgttccga
     1561 gtctaaattc agtgtggaac aaaccggtgt taccgtcagc ttctacggtg gtgctttaga
     1621 cggccagacc ttcagcaatc ccagtgatgt gaagaagttt gcccggaaag ctcagttggg
     1681 tgaaggcttc gacttcgata cggaaacctt caactctgat ggtgtattcc gcaccagtcc
     1741 ccggggttgg tttacctttg gccacgctgt ctttgccttg ctcttcttct ttggtcacat
     1801 ctggcatggt tcccggactc tgttccgtga cgtatttgct ggggttgatc ctggtttgga
     1861 agaacaagtg gaatttggtg tgtttgctaa ggttggtgac ctttccaccc ggaaagaagc
     1921 ccaccatcac catcaccatg tgtagtagct tccaggcatc aaataaaacg aaaggctcag
     1981 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2041 cacactggct caccttcggg tgggcctttc t
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