BBa_K2127004 1 CP47His CP-47-HIS TAG 2016-10-13T11:00:00Z 2016-10-21T07:31:10Z CP-47-HIS TAG CP-47-HIS TAG false false _2596_ 32415 32736 9 false CP-47-HIS TAG false Mirat Sojitra annotation2526362 1 double stop codon range2526362 1 1543 1548 annotation2526360 1 CP47His range2526360 1 1 1548 annotation2526359 1 start range2526359 1 1 3 annotation2526361 1 HisTag range2526361 1 1522 1539 BBa_K2127007 1 MaTif MT - TERMINATOR 2016-10-13T11:00:00Z 2016-10-21T05:23:57Z MT - TERMINATOR MT - TERMINATOR false false _2596_ 32415 32736 9 false MT - TERMINATOR false Mirat Sojitra BBa_K2127006 1 TFBS Transcription Factor Binding Site from Cyanobacterial Pcpc560 2016-10-13T11:00:00Z 2016-10-25T10:25:34Z Pcpc560-TFBS Pcpc560-TFBS false false _2596_ 32415 32736 9 false Pcpc560-TFBS false Mirat Sojitra annotation2530242 1 Transcription Factor Binding Sites range2530242 1 1 185 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 BBa_K2127001 1 BBa_K2127001 Inducible High Expression His-Tagged Photosystem II CP47 subunit 2016-10-13T11:00:00Z 2016-10-21T07:45:18Z Synchocisytys TFBS plac LacO CP47his false false _2596_ 0 32415 32415 9 It's complicated false synthesized false Nafaa Haddou component2527075 1 BBa_B0030 component2527066 1 BBa_K2127006 component2527067 1 BBa_R0010 component2527081 1 BBa_K2127004 component2527082 1 BBa_K2127007 annotation2527066 1 BBa_K2127006 range2527066 1 1 185 annotation2527067 1 BBa_R0010 range2527067 1 186 385 annotation2527075 1 BBa_B0030 range2527075 1 386 400 annotation2527081 1 BBa_K2127004 range2527081 1 401 1948 annotation2527082 1 BBa_K2127007 range2527082 1 1949 2071 BBa_K2127007_sequence 1 cttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct BBa_K2127006_sequence 1 cctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttg BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K2127004_sequence 1 atgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtag BBa_B0030_sequence 1 attaaagaggagaaa BBa_K2127001_sequence 1 cctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggcaacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccctaaaacataagctgaacgaaactggttgcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaattaaagaggagaaaatgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtagcttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z