##gff-version 3
##sequence-region BBa_K2127001 1 2071
BBa_K2127001	.	promoter	1	185	.	+	0	ID=BBa_K2127006;Name=BBa_K2127006
BBa_K2127001	.	non_covalent_binding_site	1	185	.	+	0	ID=annotation2530242;Name=Transcription Factor Binding Sites;Parent=BBa_K2127006
BBa_K2127001	.	terminator	1949	2071	.	+	0	ID=BBa_K2127007;Name=BBa_K2127007
BBa_K2127001	.	promoter	186	385	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K2127001	.	start_codon	358	358	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K2127001	.	CDS	186	273	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K2127001	.	promoter	346	351	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K2127001	.	non_covalent_binding_site	351	385	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K2127001	.	non_covalent_binding_site	274	311	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K2127001	.	engineered_region	186	385	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K2127001	.	promoter	322	327	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K2127001	.	ribosome_entry_site	386	400	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2127001	.	ribosome_entry_site	393	397	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2127001	.	ribosome_entry_site	386	400	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2127001	.	engineered_region	386	400	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2127001	.	CDS	401	1948	.	+	0	ID=BBa_K2127004;Name=BBa_K2127004
BBa_K2127001	.	CDS	401	1948	.	+	0	ID=annotation2526360;Name=CP47His;Parent=BBa_K2127004
BBa_K2127001	.	tag	1922	1939	.	+	0	ID=annotation2526361;Name=HisTag;Parent=BBa_K2127004
BBa_K2127001	.	start_codon	401	403	.	+	0	ID=annotation2526359;Name=start;Parent=BBa_K2127004
BBa_K2127001	.	stop_codon	1943	1948	.	+	0	ID=annotation2526362;Name=double stop codon;Parent=BBa_K2127004
>BBa_K2127001
cctgtagagaagagtccctgaatatcaaaatggtgggataaaaagctcaaaaaggaaagtaggctgtggttccctaggc
aacagtcttccctaccccactggaaactaaaaaaacgagaaaagttcgcaccgaacatcaattgcataattttagccct
aaaacataagctgaacgaaactggttgcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg
gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccc
caggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaattaaagagg
agaaaatgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaat
gcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtg
cttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggct
ggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatc
tggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaa
tccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttcc
acctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcacc
ggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggt
atcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgaga
ccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccac
cacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggtt
gatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacg
tcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggat
tggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttcccc
gtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaac
aaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtt
tgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagt
ccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccgga
ctctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttgg
tgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtagcttccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttct