LOCUS       BBa_K2127002            1886 bp    DNA     linear       22-Nov-2024
DEFINITION  CP47 His-tagged with Lac Promoter
ACCESSION   BBa_K2127002
VERSION     BBa_K2127002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             201..215
                     /label=RBS-1\Strong
     RBS             208..212
                     /label=RBS
     misc_feature    201..215
                     /label=BBa_B0030
     RBS             201..215
                     /label=BBa_B0030
     misc_feature    216..218
                     /label=start
     CDS             216..1763
                     /label=CP47His
     misc_feature    1737..1754
                     /label=HisTag
     misc_feature    1758..1763
                     /label=double stop codon
     CDS             216..1763
                     /label=BBa_K2127004
     terminator      1764..1886
                     /label=BBa_K2127007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca attaaagagg agaaaatggg actaccttgg tatcgcgttc
      241 atacagttgt cctgaatgat ccagggcgac tcatctctgt ccatttaatg cacactgccc
      301 tagtggctgg ctgggctgga tctatggctc tctatgagtt ggccattttt gactccagcg
      361 atgctgtgct taaccccatg tggcggcaag gcatgtttgt tttgcccttc atggcccgcc
      421 tcggtgtcac cagttcctgg aatggctgga gcgtcaccgg agaaactggt ttggatcccg
      481 gtttctggtc ctttgaaggg gtagctgctg cccacatcgt tctatctggt ctgttgttcc
      541 tagccgccgt atggcactgg gtattttggg acctggaatt atttgttgac ccccgtaccg
      601 gtgaatccgc attggatttg cccaaaatgt ttggtattca cctcttcttg tctggtttgc
      661 tctgcttcgg ttttggtgct ttccacctca ccggcgtttg gggccctggc atgtgggtat
      721 ccgaccccta tggtctgacg ggccacgtac agccagtggc accggaatgg ggaccagctg
      781 gctttaaccc gtttaacccc ggtggggtag tggctcacca cattgctgct ggtattgtcg
      841 gtatcattgc cggtcttttc caccttacgg tacggccccc tgagcgcttg tataaagccc
      901 tccgcatggg gaatattgag accgtattgt ccagtagtat tgccgctgta ttttttgccg
      961 ctttcgtcgt ggccggtacc atgtggtacg gtaatgccac cacccccatc gaactctttg
     1021 gccccacccg ttatcagtgg gataagggtt attttcaaga agaaatccaa cgccgggttg
     1081 atagccagtt agcagaagga gcttccctat ctgaagcctg gtccaccatc cctgaaaagt
     1141 tggctttcta cgattacgtc ggtaacagcc ccgctaaagg tggtttgttt cgtaccggtg
     1201 ctatgaacag tggtgatggc attgcccagg aatggattgg tcaccccata ttcaaagaca
     1261 aagaaggtcg ggaactggag gtacggcgta tgcctaactt ctttgaaact ttccccgtca
     1321 tcatgaccga tgcggatggt gtagtccggg cggatattcc cttccgtcgt tccgagtcta
     1381 aattcagtgt ggaacaaacc ggtgttaccg tcagcttcta cggtggtgct ttagacggcc
     1441 agaccttcag caatcccagt gatgtgaaga agtttgcccg gaaagctcag ttgggtgaag
     1501 gcttcgactt cgatacggaa accttcaact ctgatggtgt attccgcacc agtccccggg
     1561 gttggtttac ctttggccac gctgtctttg ccttgctctt cttctttggt cacatctggc
     1621 atggttcccg gactctgttc cgtgacgtat ttgctggggt tgatcctggt ttggaagaac
     1681 aagtggaatt tggtgtgttt gctaaggttg gtgacctttc cacccggaaa gaagcccacc
     1741 atcaccatca ccatgtgtag tagcttccag gcatcaaata aaacgaaagg ctcagtcgaa
     1801 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1861 tggctcacct tcgggtgggc ctttct
//