##gff-version 3 ##sequence-region BBa_K2127002 1 1886 BBa_K2127002 . terminator 1764 1886 . + 0 ID=BBa_K2127007;Name=BBa_K2127007 BBa_K2127002 . ribosome_entry_site 201 215 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_K2127002 . ribosome_entry_site 201 215 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_K2127002 . engineered_region 201 215 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_K2127002 . ribosome_entry_site 208 212 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_K2127002 . CDS 216 1763 . + 0 ID=BBa_K2127004;Name=BBa_K2127004 BBa_K2127002 . stop_codon 1758 1763 . + 0 ID=annotation2526362;Name=double stop codon;Parent=BBa_K2127004 BBa_K2127002 . start_codon 216 218 . + 0 ID=annotation2526359;Name=start;Parent=BBa_K2127004 BBa_K2127002 . CDS 216 1763 . + 0 ID=annotation2526360;Name=CP47His;Parent=BBa_K2127004 BBa_K2127002 . tag 1737 1754 . + 0 ID=annotation2526361;Name=HisTag;Parent=BBa_K2127004 BBa_K2127002 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K2127002 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K2127002 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K2127002 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K2127002 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K2127002 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K2127002 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K2127002 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 >BBa_K2127002 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacaattaaagaggagaaaatgggactaccttggtatcgcg ttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggc tggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatg tttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttgg atcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatg gcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgttt ggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggca tgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaaccc gtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacg gtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctg tattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccac ccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttcc ctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtt tgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaaga aggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgta gtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacg gtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaagg cttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccac gctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctgggg ttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagccca ccatcaccatcaccatgtgtagtagcttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct