BBa_K2127004
1
CP47His
CP-47-HIS TAG
2016-10-13T11:00:00Z
2016-10-21T07:31:10Z
CP-47-HIS TAG
CP-47-HIS TAG
false
false
_2596_
32415
32736
9
false
CP-47-HIS TAG
false
Mirat Sojitra
annotation2526362
1
double stop codon
range2526362
1
1543
1548
annotation2526359
1
start
range2526359
1
1
3
annotation2526360
1
CP47His
range2526360
1
1
1548
annotation2526361
1
HisTag
range2526361
1
1522
1539
BBa_K2127007
1
MaTif
MT - TERMINATOR
2016-10-13T11:00:00Z
2016-10-21T05:23:57Z
MT - TERMINATOR
MT - TERMINATOR
false
false
_2596_
32415
32736
9
false
MT - TERMINATOR
false
Mirat Sojitra
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
BBa_K2127002
1
BBa_K2127002
CP47 His-tagged with Lac Promoter
2016-10-13T11:00:00Z
2016-10-21T07:46:10Z
NCBI Reference Sequence: NC_000911.1
CP47 His-tagged with Lac Promoter synthesized by IDT DNA Inc.
false
false
_2596_
32415
17480
9
false
Double Stop Codon.
ORF Check
false
Dawson Zeng
component2527099
1
BBa_R0010
component2527113
1
BBa_K2127004
component2527114
1
BBa_K2127007
component2527107
1
BBa_B0030
annotation2527113
1
BBa_K2127004
range2527113
1
216
1763
annotation2527114
1
BBa_K2127007
range2527114
1
1764
1886
annotation2527099
1
BBa_R0010
range2527099
1
1
200
annotation2527107
1
BBa_B0030
range2527107
1
201
215
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K2127007_sequence
1
cttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K2127004_sequence
1
atgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtag
BBa_K2127002_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaattaaagaggagaaaatgggactaccttggtatcgcgttcatacagttgtcctgaatgatccagggcgactcatctctgtccatttaatgcacactgccctagtggctggctgggctggatctatggctctctatgagttggccatttttgactccagcgatgctgtgcttaaccccatgtggcggcaaggcatgtttgttttgcccttcatggcccgcctcggtgtcaccagttcctggaatggctggagcgtcaccggagaaactggtttggatcccggtttctggtcctttgaaggggtagctgctgcccacatcgttctatctggtctgttgttcctagccgccgtatggcactgggtattttgggacctggaattatttgttgacccccgtaccggtgaatccgcattggatttgcccaaaatgtttggtattcacctcttcttgtctggtttgctctgcttcggttttggtgctttccacctcaccggcgtttggggccctggcatgtgggtatccgacccctatggtctgacgggccacgtacagccagtggcaccggaatggggaccagctggctttaacccgtttaaccccggtggggtagtggctcaccacattgctgctggtattgtcggtatcattgccggtcttttccaccttacggtacggccccctgagcgcttgtataaagccctccgcatggggaatattgagaccgtattgtccagtagtattgccgctgtattttttgccgctttcgtcgtggccggtaccatgtggtacggtaatgccaccacccccatcgaactctttggccccacccgttatcagtgggataagggttattttcaagaagaaatccaacgccgggttgatagccagttagcagaaggagcttccctatctgaagcctggtccaccatccctgaaaagttggctttctacgattacgtcggtaacagccccgctaaaggtggtttgtttcgtaccggtgctatgaacagtggtgatggcattgcccaggaatggattggtcaccccatattcaaagacaaagaaggtcgggaactggaggtacggcgtatgcctaacttctttgaaactttccccgtcatcatgaccgatgcggatggtgtagtccgggcggatattcccttccgtcgttccgagtctaaattcagtgtggaacaaaccggtgttaccgtcagcttctacggtggtgctttagacggccagaccttcagcaatcccagtgatgtgaagaagtttgcccggaaagctcagttgggtgaaggcttcgacttcgatacggaaaccttcaactctgatggtgtattccgcaccagtccccggggttggtttacctttggccacgctgtctttgccttgctcttcttctttggtcacatctggcatggttcccggactctgttccgtgacgtatttgctggggttgatcctggtttggaagaacaagtggaatttggtgtgtttgctaaggttggtgacctttccacccggaaagaagcccaccatcaccatcaccatgtgtagtagcttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
BBa_B0030_sequence
1
attaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z