BBa_K2127007
1
MaTif
MT - TERMINATOR
2016-10-13T11:00:00Z
2016-10-21T05:23:57Z
MT - TERMINATOR
MT - TERMINATOR
false
false
_2596_
32415
32736
9
false
MT - TERMINATOR
false
Mirat Sojitra
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K2127003
1
BBa_K2127003
psbT
2016-10-13T11:00:00Z
2016-10-25T05:51:22Z
synchocystis
psbT
false
false
_2596_
32415
32415
9
false
coding
false
Nafaa Haddou
component2526861
1
BBa_K2127005
component2526848
1
BBa_R0010
component2526856
1
BBa_B0032
component2526862
1
BBa_K2127007
annotation2526848
1
BBa_R0010
range2526848
1
1
200
annotation2526862
1
BBa_K2127007
range2526862
1
433
555
annotation2526856
1
BBa_B0032
range2526856
1
201
213
annotation2526861
1
BBa_K2127005
range2526861
1
214
432
BBa_K2127005
1
BBa_K2127005
psbT
2016-10-13T11:00:00Z
2016-10-21T05:03:14Z
BBa_K2127005
BBa_K2127005
false
false
_2596_
32736
32736
9
false
BBa_K2127005
false
Mirat Sojitra
annotation2526422
1
psbT
range2526422
1
1
219
annotation2526424
1
double stop codon
range2526424
1
215
219
annotation2526423
1
start
range2526423
1
1
3
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
BBa_K2127007_sequence
1
cttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K2127003_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatcacacaggaaagatgggcgaatcaaagcgtcgtaaacaatcccttggcgatcgctatggccaggatcaacgggttgtggagtggctgcctttaaccaaaaaacaagccgaagatttctataaatggaccaccaaaggagcttggattggcatcggtgttatggcaggcctgtgggttacagtcagatttattggccccacttttggctggtggaccgtccaataatagtagcttccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
BBa_K2127005_sequence
1
atgggcgaatcaaagcgtcgtaaacaatcccttggcgatcgctatggccaggatcaacgggttgtggagtggctgcctttaaccaaaaaacaagccgaagatttctataaatggaccaccaaaggagcttggattggcatcggtgttatggcaggcctgtgggttacagtcagatttattggccccacttttggctggtggaccgtccaataatagtag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z