BBa_K2128006 1 BBa_K2128006 RFP with H. Dujardini ACTN Promoter, RBS and Terminator 2016-08-12T11:00:00Z 2016-10-18T02:26:13Z BBa_E1010, H. Dujardini This part encodes for a Red Fluorescent Protein in H. Dujardini and potentially other Tardigrade species. The RFP in this part was taken from BBa_E1010, a "highly engineered mutant of red fluorescent protein from Discosoma striata". It was codon optimized Optimized for C. Elegans by IDT's Codon Optimization Tool. The promoter, RBS and Terminator where taken from the H. Dujardini ACTN gene. It is a constitutive promoter that is on at all stages of development. false false _2597_ 32302 32302 9 false We codon optimized the RFP ORF for C. Elegans as no optimization for H. Dujardini or other Tardigrade species is available. false David Sanderson component2518490 1 BBa_K2128008 component2518493 1 BBa_K2128009 component2518492 1 BBa_K2128010 annotation2518493 1 BBa_K2128009 range2518493 1 1054 1353 annotation2518492 1 BBa_K2128010 range2518492 1 368 1045 annotation2518490 1 BBa_K2128008 range2518490 1 1 361 BBa_K2128010 1 BBa_K2128010 RFP Optimzed for C. Elegans 2016-10-17T11:00:00Z 2016-10-18T01:49:33Z Discosoma striata This is a RFP encoding ORF, it was taken from BBa_E1010, a "highly engineered mutant of red fluorescent protein from Discosoma striata". It was codon optimized for C. Elegans by IDT's Codon Optimization Tool. false false _2597_ 32302 32302 9 false It was codon optimized for C. Elegans by IDT's Codon Optimization Tool. false David Sanderson annotation2518326 1 RFP range2518326 1 1 678 BBa_K2128009 1 BBa_K2128009 H. Dujardini ACTN Termanator w/ 3' UTR 2016-10-17T11:00:00Z 2016-10-18T01:39:13Z H. Dujardini Terminator of the ACTN gene in the tardigrade Hypsibius dujardini. Actin is a protein involved in the formation of the filaments of muscle cells as well as cell mobility and contraction. false false _2597_ 32302 32302 9 false Includes the 3' UTR. false David Sanderson BBa_K2128008 1 BBa_K2128008 H. dujardini ACTN promoter w/ 5' UTR 2016-09-21T11:00:00Z 2016-10-18T01:29:18Z Hypsibius dujardini Constitutive promoter of the actin gene in the tardigrade Hypsibius dujardini. Actin is a protein involved in the formation of the filaments of muscle cells as well as cell mobility and contraction. false false _2597_ 32302 30412 9 false Sequence was taken by identifying the actin gene in the genome of Hypsibius dujardini. 500 bp upstream of the actin start codon was taken and subsequently ran through a promoter prediction tool which can be found at fruitfly.org. false Sunil Deochand, David Sanderson BBa_K2128010_sequence 1 atggcttcgtcagaagacgttattaaggagttcatgcgttttaaggtaagaatggaaggctccgttaacggccacgaatttgagattgagggcgaaggagaaggtagaccgtatgaaggcactcagacagctaagttaaaagtgacgaagggaggccctctcccttttgcctgggatatattatctccacaatttcagtatggttcaaaggcatacgtcaaacatccagctgacattccagattatttaaagctctcatttcctgaaggctttaagtgggagcgtgttatgaacttcgaggacggcggagtggttacagtgacccaagactcgtcactccaggacggtgaattcatctacaaagtcaagttgcgtggcacgaatttcccatcggatggaccggtcatgcaaaaaaagactatgggctgggaggcatcgacagaacgtatgtacccggaggacggtgcgttaaagggtgaaatcaaaatgcgtttgaagttaaaggatggcggtcattacgatgcggaagttaaaacgacctacatggcaaagaagccagtccagcttccgggagcttacaaaacggacatcaagttggacatcacttcccataacgaggactataccattgtagagcagtacgagcgtgcggagggtagacactctacgggtgcctga BBa_K2128006_sequence 1 gttggcgtacaggtccttgcggatatcaatgtcgcacttcatgatcgagttgtaggtggtctcgtggataccgcacgactccatacctgtcggcagacaacggagaaaacgtgaaaagacgggccaaacacaggcgcaaggtccgaatagtcgggctgactggacgctgtctgtctgtctgcctcaattcttaccgatgaagctgggttggaagagggcctcggggcatcggaatcgctcgtttccgatggtgatgacttgaccgtcgggaagttcgtagctcttctccagggaggacgaggcagcagcggtggccatttcctgctcaaagtcgagggcgacgtagcagagcttctccttgtactagatggcttcgtcagaagacgttattaaggagttcatgcgttttaaggtaagaatggaaggctccgttaacggccacgaatttgagattgagggcgaaggagaaggtagaccgtatgaaggcactcagacagctaagttaaaagtgacgaagggaggccctctcccttttgcctgggatatattatctccacaatttcagtatggttcaaaggcatacgtcaaacatccagctgacattccagattatttaaagctctcatttcctgaaggctttaagtgggagcgtgttatgaacttcgaggacggcggagtggttacagtgacccaagactcgtcactccaggacggtgaattcatctacaaagtcaagttgcgtggcacgaatttcccatcggatggaccggtcatgcaaaaaaagactatgggctgggaggcatcgacagaacgtatgtacccggaggacggtgcgttaaagggtgaaatcaaaatgcgtttgaagttaaaggatggcggtcattacgatgcggaagttaaaacgacctacatggcaaagaagccagtccagcttccgggagcttacaaaacggacatcaagttggacatcacttcccataacgaggactataccattgtagagcagtacgagcgtgcggagggtagacactctacgggtgcctgatactagaggttcatggttcatacctgggtcatcttttccctgttggccttggggttgaggggagcctcagtcaggaggacggggtgttcctcgggagccacgcggagctcgttgtagaaggtgtgatgccagatcttctccatgtcatcccagttggtgacgatgccgtgctcgatggggtacttgagcgtcaggataccgcgcttgctctgggcctcatcaccgacgtagctgtccttttgacccataccgaccatgacaccctacaaggtaacacggaggcaaatgtcaatcagtcacacccagca BBa_K2128008_sequence 1 gttggcgtacaggtccttgcggatatcaatgtcgcacttcatgatcgagttgtaggtggtctcgtggataccgcacgactccatacctgtcggcagacaacggagaaaacgtgaaaagacgggccaaacacaggcgcaaggtccgaatagtcgggctgactggacgctgtctgtctgtctgcctcaattcttaccgatgaagctgggttggaagagggcctcggggcatcggaatcgctcgtttccgatggtgatgacttgaccgtcgggaagttcgtagctcttctccagggaggacgaggcagcagcggtggccatttcctgctcaaagtcgagggcgacgtagcagagcttctccttg BBa_K2128009_sequence 1 gttcatggttcatacctgggtcatcttttccctgttggccttggggttgaggggagcctcagtcaggaggacggggtgttcctcgggagccacgcggagctcgttgtagaaggtgtgatgccagatcttctccatgtcatcccagttggtgacgatgccgtgctcgatggggtacttgagcgtcaggataccgcgcttgctctgggcctcatcaccgacgtagctgtccttttgacccataccgaccatgacaccctacaaggtaacacggaggcaaatgtcaatcagtcacacccagca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z