BBa_K2128011
1
BBa_K2128011
GFP Optimzed for C. Elegans
2016-10-17T11:00:00Z
2016-10-18T02:16:38Z
A. victoria
This part encodes for a Green Fluorescent Protein. The GFP in this part was codon optimized for C. Elegans by IDT's Codon Optimization Tool.
false
false
_2597_
32302
32302
9
false
codon optimized for C. Elegans by IDT's Codon Optimization Tool.
false
David Sanderson
BBa_K2128007
1
BBa_K2128007
GFP with H. Dujardini ACTN Promoter, RBS and Terminator
2016-08-12T11:00:00Z
2016-10-18T02:34:28Z
H. Dujardini, GFP originally from Aequorea victoria.
This part encodes for a Green Fluorescent Protein in H. Dujardini and potentially other Tardigrade species.
The GFP in this part was codon optimized Optimized for C. Elegans by IDT's Codon Optimization Tool.
The promoter, RBS and Terminator were taken from the H. Dujardini ACTN gene. It is a constitutive promoter that is on at all stages of development.
false
false
_2597_
32302
32302
9
false
We codon optimized the RFP ORF for C. Elegans as no optimization for H. Dujardini or other Tardigrade species is available.
false
David Sanderson
component2518518
1
BBa_K2128011
component2518517
1
BBa_K2128008
component2518519
1
BBa_K2128009
annotation2518518
1
BBa_K2128011
range2518518
1
368
1087
annotation2518517
1
BBa_K2128008
range2518517
1
1
361
annotation2518519
1
BBa_K2128009
range2518519
1
1096
1395
BBa_K2128009
1
BBa_K2128009
H. Dujardini ACTN Termanator w/ 3' UTR
2016-10-17T11:00:00Z
2016-10-18T01:39:13Z
H. Dujardini
Terminator of the ACTN gene in the tardigrade Hypsibius dujardini. Actin is a protein involved in the formation of the filaments of muscle cells as well as cell mobility and contraction.
false
false
_2597_
32302
32302
9
false
Includes the 3' UTR.
false
David Sanderson
BBa_K2128008
1
BBa_K2128008
H. dujardini ACTN promoter w/ 5' UTR
2016-09-21T11:00:00Z
2016-10-18T01:29:18Z
Hypsibius dujardini
Constitutive promoter of the actin gene in the tardigrade Hypsibius dujardini. Actin is a protein involved in the formation of the filaments of muscle cells as well as cell mobility and contraction.
false
false
_2597_
32302
30412
9
false
Sequence was taken by identifying the actin gene in the genome of Hypsibius dujardini. 500 bp upstream of the actin start codon was taken and subsequently ran through a promoter prediction tool which can be found at fruitfly.org.
false
Sunil Deochand, David Sanderson
BBa_K2128011_sequence
1
atggtcagtaaaggtgaggaactttttacgggcgttgttccaatcctggtggaattggacggtgatgtaaacggacacaagttttcggtatcaggtgaaggtgagggtgatgcaacgtacggcaagttgacattaaagttcatatgtacaacaggtaagctgccggtcccttggcctaccttagtaactacgctgacttacggtgttcagtgtttctcgcgttacccagaccacatgaagcagcacgatttctttaagtccgccatgccagagggatatgttcaggaaagaaccatcttttttaaggacgatggcaattacaaaacccgagcggaggtcaaattcgagggtgacacattagtgaatcgaatagagttaaagggaattgactttaaagaagacggcaacattttaggtcacaagttagagtataattacaactcacataacgtctatatcatggctgacaaacagaaaaatggtatcaaggccaactttaagatcagacataacatcgaagatggaagtgttcaactggctgatcattatcaacaaaacacccctataggtgatggtccggtgttgcttcctgataaccactatctttccacccaatctgccttgtctaaggatccaaatgaaaagagagatcacatggtattactggagtttgtaacagcggctggaattaccttaggaatggacgaactctacaaataa
BBa_K2128007_sequence
1
gttggcgtacaggtccttgcggatatcaatgtcgcacttcatgatcgagttgtaggtggtctcgtggataccgcacgactccatacctgtcggcagacaacggagaaaacgtgaaaagacgggccaaacacaggcgcaaggtccgaatagtcgggctgactggacgctgtctgtctgtctgcctcaattcttaccgatgaagctgggttggaagagggcctcggggcatcggaatcgctcgtttccgatggtgatgacttgaccgtcgggaagttcgtagctcttctccagggaggacgaggcagcagcggtggccatttcctgctcaaagtcgagggcgacgtagcagagcttctccttgtactagatggtcagtaaaggtgaggaactttttacgggcgttgttccaatcctggtggaattggacggtgatgtaaacggacacaagttttcggtatcaggtgaaggtgagggtgatgcaacgtacggcaagttgacattaaagttcatatgtacaacaggtaagctgccggtcccttggcctaccttagtaactacgctgacttacggtgttcagtgtttctcgcgttacccagaccacatgaagcagcacgatttctttaagtccgccatgccagagggatatgttcaggaaagaaccatcttttttaaggacgatggcaattacaaaacccgagcggaggtcaaattcgagggtgacacattagtgaatcgaatagagttaaagggaattgactttaaagaagacggcaacattttaggtcacaagttagagtataattacaactcacataacgtctatatcatggctgacaaacagaaaaatggtatcaaggccaactttaagatcagacataacatcgaagatggaagtgttcaactggctgatcattatcaacaaaacacccctataggtgatggtccggtgttgcttcctgataaccactatctttccacccaatctgccttgtctaaggatccaaatgaaaagagagatcacatggtattactggagtttgtaacagcggctggaattaccttaggaatggacgaactctacaaataatactagaggttcatggttcatacctgggtcatcttttccctgttggccttggggttgaggggagcctcagtcaggaggacggggtgttcctcgggagccacgcggagctcgttgtagaaggtgtgatgccagatcttctccatgtcatcccagttggtgacgatgccgtgctcgatggggtacttgagcgtcaggataccgcgcttgctctgggcctcatcaccgacgtagctgtccttttgacccataccgaccatgacaccctacaaggtaacacggaggcaaatgtcaatcagtcacacccagca
BBa_K2128008_sequence
1
gttggcgtacaggtccttgcggatatcaatgtcgcacttcatgatcgagttgtaggtggtctcgtggataccgcacgactccatacctgtcggcagacaacggagaaaacgtgaaaagacgggccaaacacaggcgcaaggtccgaatagtcgggctgactggacgctgtctgtctgtctgcctcaattcttaccgatgaagctgggttggaagagggcctcggggcatcggaatcgctcgtttccgatggtgatgacttgaccgtcgggaagttcgtagctcttctccagggaggacgaggcagcagcggtggccatttcctgctcaaagtcgagggcgacgtagcagagcttctccttg
BBa_K2128009_sequence
1
gttcatggttcatacctgggtcatcttttccctgttggccttggggttgaggggagcctcagtcaggaggacggggtgttcctcgggagccacgcggagctcgttgtagaaggtgtgatgccagatcttctccatgtcatcccagttggtgacgatgccgtgctcgatggggtacttgagcgtcaggataccgcgcttgctctgggcctcatcaccgacgtagctgtccttttgacccataccgaccatgacaccctacaaggtaacacggaggcaaatgtcaatcagtcacacccagca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z