BBa_K2129002 1 BBa_K2129002 Mutant EutS with Mid expression cassette 2016-10-12T11:00:00Z 2016-11-03T06:06:12Z EutS derived from K2129001, mutated with site directed mutagenesis (QuikChange). EutS with residue G26 replaced with an Amber stop codon for incorporation of non-canonical amino acids with a custom tRNA synthetase. false false _2598_ 31623 31623 9 false G26 was selected based on predictive modelling of EutS superstructure to incorporate at a locus where a phenylalanine-4'-azobenzene non-canonical amino acid might be able to hinder superstructure formation when under cis or trans conditions. false Maxwell Saal BBa_K2129002_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagtttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaataaagaacgcattattcaggaatttgtgccgggcaaacaggtcacgctggcgcatctcattgcgcacccttaggaagaactggcgaaaaagatcggcgttcccgatgctggcgcgattggcattatgactttaacgccgggggaaacggcgatgatcgctggcgatttagcgatgaaggctgcggatgtacacatcggttttctcgatagatttagcggcgcgctggtgatttatggcaccgtcggcgcagtagaagaagcgctattacagacggtcagcgggctggggcgattattaaactttaccctgtgtgagttaacaaaaagttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z