LOCUS BBa_K2130000 3534 bp DNA linear 26-Nov-2024 DEFINITION ∆RuvCIII-2 ∆HNH Sp-dCas9 ACCESSION BBa_K2130000 VERSION BBa_K2130000.1 FEATURES Location/Qualifiers misc_feature 2327..2338 /label=GGGS linker misc_feature 2618..2641 /label=(GGGS)2 linker ORIGIN 1 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc 61 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc 121 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa 181 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc 241 taccttcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg 301 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc 361 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag 421 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat 481 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 541 gtcgataaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg 601 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg 661 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat 721 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa 781 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc 841 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt 901 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt 961 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga 1021 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc 1081 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg 1141 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc 1201 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac 1261 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt 1321 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc 1381 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa 1441 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa 1501 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt 1561 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg 1621 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc 1681 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc 1741 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc 1801 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc 1861 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct 1921 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg 1981 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg 2041 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac 2101 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt 2161 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat acttcagacc 2221 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt 2281 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagggtgg tggttctgag 2341 ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat caccaagcac 2401 gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga caaactgatt 2461 cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag aaaggacttt 2521 cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc ctacctgaat 2581 gcagtggtag gcactgcact tatcaaaaaa tatcccggtg gtggttctgg tggtggttct 2641 gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc 2701 gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg 2761 aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc 2821 gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct 2881 aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc 2941 gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata aagaggtcaa aaaagacctc 3001 atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc 3061 gctagtgcgg gcgagcttca gaaaggtaac gagctggcac tgccctctaa atacgttaat 3121 ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag 3181 aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc 3241 gagttctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac 3301 aataagcaca gggataagcc catcagggag caggcagaaa acattatcca cttgtttact 3361 ctgaccaact tgggcgcgcc tgctgccttc aagtacttcg acaccaccat agacagaaag 3421 cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg 3481 ctctatgaaa caagaatcga cctctctcag ctcggtggag acagcagggc tgac //