LOCUS BBa_K2130001 4110 bp DNA linear 23-Dec-2024 DEFINITION Cas9 mutant 462 ACCESSION BBa_K2130001 VERSION BBa_K2130001.1 FEATURES Location/Qualifiers misc_feature 2607..2607 /label=N869K misc_feature 2710..2710 /label=E904K ORIGIN 1 atggataaaa agtattctat tggtttagac atcggcacta attccgttgg atgggctgtc 61 ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt 121 cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag 181 gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt 241 tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt 301 ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga 361 aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa 421 aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat 481 atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat 541 gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct 601 ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga 661 cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac 721 cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa 781 gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca 841 caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc 901 ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca 961 atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt 1021 cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca 1081 ggttatattg acggcggagc gagtcaagag gagttctaca agtttatcaa acccatatta 1141 gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga 1201 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat 1261 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt 1321 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct 1381 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccctggaa ttttgaggaa 1441 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag 1501 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg 1561 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta 1621 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca 1681 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc 1741 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata 1801 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg 1861 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct 1921 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga 1981 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc 2041 gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac 2101 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg 2161 cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca 2221 gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta 2281 atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg 2341 atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct 2401 gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg 2461 gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac 2521 attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg 2581 gataagaacc gagggaaaag tgacaaagtt ccaagcgagg aagtcgtaaa gaaaatgaag 2641 aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta 2701 actaaagcta agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag 2761 ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat 2821 acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca 2881 aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac 2941 taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa 3001 tacccgaagc tagaaagtga atttgtgtat ggtgattaca aagtttatga cgtccgtaag 3061 atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct 3121 aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga 3181 cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc 3241 gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg 3301 cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc 3361 gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc 3421 tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc 3481 aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac 3541 ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag 3601 tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt 3661 caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc 3721 cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag 3781 cagcacaaac attatctcga cgaaatcata gagcaaattt cggagttcag taagagagtc 3841 atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa 3901 cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct 3961 ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag 4021 gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata 4081 gatttgtcac agcttggggg tgacggatcc //