LOCUS BBa_K2130005 3324 bp DNA linear 25-Dec-2024 DEFINITION NmCas9 ACCESSION BBa_K2130005 VERSION BBa_K2130005.1 FEATURES Location/Qualifiers CDS 3277..3324 /label=NLS CDS 7..27 /label=NLS misc_feature 28..3270 /label=Nm-Cas9 ORIGIN 1 atggtgccta agaagaagag aaaggtggct gccttcaaac ctaattcaat caactacatc 61 ctcggcctcg atatcggcat cgcatccgtc ggctgggcga tggtagaaat tgacgaagaa 121 gaaaacccca tccgcctgat tgatttgggc gtgcgcgtat ttgagcgtgc cgaagtaccg 181 aaaacaggcg actcccttgc catggcaagg cgtttggcgc gcagtgttcg ccgcctgacc 241 cgccgtcgcg cccaccgcct gcttcggacc cgccgcctat tgaaacgcga aggcgtatta 301 caagccgcca attttgacga aaacggcttg attaaatcct taccgaatac accatggcaa 361 cttcgcgcag ccgcattaga ccgcaaactg acgcctttag agtggtcggc agtcttgttg 421 catttaatca aacatcgcgg ctatttatcg caacggaaaa acgagggcga aactgccgat 481 aaggagcttg gcgctttgct taaaggcgta gccggcaatg cccatgcctt acagacaggc 541 gatttccgca caccggccga attggcttta aataaatttg agaaagaaag cggccatatc 601 cgcaatcagc gcagcgatta ttcgcatacg ttcagccgca aagatttaca ggcggagctg 661 attttgctgt ttgaaaaaca aaaagaattt ggcaatccgc atgtttcagg cggccttaaa 721 gaaggtattg aaaccctact gatgacgcaa cgccctgccc tgtccggcga tgccgttcaa 781 aaaatgttgg ggcattgcac cttcgaaccg gcagagccga aagccgctaa aaacacctac 841 acagccgaac gtttcatctg gctgaccaag ctgaacaacc tgcgtatttt agagcaaggc 901 agcgagcggc cattgaccga taccgaacgc gccacgctta tggacgagcc atacagaaaa 961 tccaaactga cttacgcaca agcccgtaag ctgctgggtt tagaagatac cgcctttttc 1021 aaaggcttgc gctatggtaa agacaatgcc gaagcctcaa cattgatgga aatgaaggcc 1081 taccatgcca tcagccgtgc actggaaaaa gaaggattga aagacaaaaa atccccatta 1141 aacctttctc ccgaattaca agacgaaatc ggcacggcat tctccctgtt caaaaccgat 1201 gaagacatta caggccgtct gaaagaccgt atacagcccg aaatcttaga agcgctgttg 1261 aaacacatca gcttcgataa gttcgtccaa atttccttga aagcattgcg ccgaattgtg 1321 cctctaatgg aacaaggcaa acgttacgat gaagcctgcg ccgaaatcta cggagaccat 1381 tacggcaaga agaatacgga agaaaagatt tatctgccgc cgattcccgc cgacgaaatc 1441 cgcaaccccg tcgtcttgcg cgccttatct caagcacgta aggtcattaa cggcgtggta 1501 cgccgttacg gctccccagc tcgtatccat attgaaactg caagggaagt aggtaaatcg 1561 tttaaagacc gcaaagaaat tgagaaacgc caagaagaaa accgcaaaga ccgggaaaaa 1621 gccgccgcca aattccgaga gtatttcccc aattttgtcg gagaacccaa atccaaagat 1681 attctgaaac tgcgcctgta cgagcaacaa cacggcaaat gcctgtattc gggcaaagaa 1741 atcaacttag gccgtctgaa cgaaaaaggc tatgtcgaaa tcgaccatgc cctgccgttc 1801 tcgcgcacat gggacgacag tttcaacaat aaagtactgg tattgggcag cgaaaaccaa 1861 aacaaaggca atcaaacccc ttacgaatac ttcaacggca aagacaacag ccgcgaatgg 1921 caggaattta aagcgcgtgt cgaaaccagc cgtttcccgc gcagtaaaaa acaacggatt 1981 ctgctgcaaa aattcgatga agacggcttt aaagaacgca atctgaacga cacgcgctac 2041 gtcaaccgtt tcctgtgtca atttgttgcc gaccgtatgc ggctgacagg taaaggcaag 2101 aaacgtgtct ttgcatccaa cggacaaatt accaatctgt tgcgcggctt ttggggattg 2161 cgcaaagtgc gtgcggaaaa cgaccgccat cacgccttgg acgccgtcgt cgttgcctgc 2221 tcgaccgttg ccatgcagca gaaaattacc cgttttgtac gctataaaga gatgaacgcg 2281 tttgacggta aaaccataga caaagaaaca ggagaagtgc tgcatcaaaa aacacacttc 2341 ccacaacctt gggaattttt cgcacaagaa gtcatgattc gcgtcttcgg caaaccggac 2401 ggcaaacccg aatttgaaga agccgatacc ctagaaaaac tgcgcacgtt gcttgccgaa 2461 aaattatcat ctcgccccga agccgtacac gaatacgtta cgccactgtt tgtttcacgc 2521 gcgcccaatc ggaagatgag cgggcaaggg catatggaga ccgtcaaatc cgccaaacga 2581 ctggacgaag gcgtcagcgt gttgcgcgta ccgctgacac agttaaaact gaaagacttg 2641 gaaaaaatgg tcaatcggga gcgcgaacct aagctatacg aagcactgaa agcacggctg 2701 gaagcacata aagacgatcc tgccaaagcc tttgccgagc cgttttacaa atacgataaa 2761 gcaggcaacc gcacccaaca ggtaaaagcc gtacgcgtag agcaagtaca gaaaaccggc 2821 gtatgggtgc gcaaccataa cggtattgcc gacaacgcaa ccatggtgcg cgtagatgtg 2881 tttgagaaag gcgacaagta ttatctggta ccgatttaca gttggcaggt agcgaaaggg 2941 attttgccgg atagggctgt tgtacaagga aaagatgaag aagattggca acttattgat 3001 gatagtttca actttaaatt ctcattacac cctaatgatt tagtcgaggt tataacaaaa 3061 aaagctagaa tgtttggtta ctttgccagc tgccatcgag gcacaggtaa tatcaatata 3121 cgcattcatg atcttgatca taaaattggc aaaaatggaa tactggaagg tatcggcgtc 3181 aaaaccgccc tttcattcca aaaataccaa attgacgaac tgggcaaaga aatcagacca 3241 tgccgtctga aaaaacgccc gcctgtccgt accggtaaaa ggccggcggc cacgaaaaag 3301 gccggccagg caaaaaagaa aaag //