BBa_K2130008 1 BBa_K2130008 LbCpf1 gRNA expression cassette 2016-10-18T11:00:00Z 2016-10-19T12:51:48Z Addgene The gRNA is expressed by the hU6 promoter. To insert gRNA, digest with BsmBI. To ligate in gRNA: Design Top strand of your spacer with 5' AGAT overhang, bottom strand with 5'AAAA overhang. Example: 5' AGATNNNNNNNNNNNNNNNN 3' 3' NNNNNNNNNNNNNNNNAAAA 5' false false _2599_ 25361 25361 9 false NA false Ooi Kean Hean BBa_K2130008_sequence 1 aggtcgggcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgtaatttctactaagtgtagatggagacgattaatgcgtctcgtttttttaagcttgggccgctcgagctagtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z