LOCUS BBa_K2130009 3342 bp DNA linear 26-Nov-2024 DEFINITION ∆HNH ∆REC2 SP-dCas9 ACCESSION BBa_K2130009 VERSION BBa_K2130009.1 FEATURES Location/Qualifiers misc_feature 1949..1960 /label=GGGS linker ORIGIN 1 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc 61 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc 121 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa 181 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc 241 taccttcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg 301 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc 361 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag 421 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat 481 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 541 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac 601 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag 661 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc 721 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag 781 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga 841 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat 901 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata 961 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa 1021 tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc 1081 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt 1141 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa 1201 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc 1261 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagactat 1321 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac 1381 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac 1441 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat 1501 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg 1561 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat 1621 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt 1681 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc 1741 cagaaagcac aagtttctgg ccagggggac agtcttcacg agcacatcgc taatcttgca 1801 ggtagcccag ctatcaaaaa gggaatactt cagaccgtta aggtcgtgga tgaactcgtc 1861 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa 1921 actacccaga agggacagaa gggtggtggt tctgagttgg ataaagccgg cttcatcaaa 1981 aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc 2041 atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg 2101 aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc 2161 aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc 2221 aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt 2281 aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt 2341 tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg 2401 aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg 2461 gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc 2521 gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag 2581 ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca 2641 gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa 2701 agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc 2761 atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt 2821 cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc 2881 gagcttcaga aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg 2941 gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc 3001 gtggaacaac acaaacacta ccttgatgag atcatcgagc aaataagcga gttctccaaa 3061 agagtgatcc tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg 3121 gataagccca tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg 3181 ggcgcgcctg ctgccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct 3241 acaaaggagg tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca 3301 agaatcgacc tctctcagct cggtggagac agcagggctg ac //