BBa_K2130010 1 BBa_K2130010 ∆REC1-3 SP-dCas9 2016-10-18T11:00:00Z 2016-10-19T06:19:11Z Addgene dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. For this part, we have deleted the HNH domain of the SP-dCas9 and tested it's binding capability by assessing the strength in transcriptional activation via a VP64-p65-Rta. http://2016.igem.org/wiki/images/d/df/T--NTU-Singapore--rec1.png http://2016.igem.org/wiki/images/1/1e/T--NTU-Singapore--rec1del.png http://2016.igem.org/wiki/images/b/bb/T--NTU-Singapore--rec1delresult.png http://2016.igem.org/wiki/images/8/81/T--NTU-Singapore--rec1micro.jpg false false _2599_ 25361 25361 9 false created by Gibson Assemly false Ooi Kean Hean igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z