LOCUS BBa_K2130012 3726 bp DNA linear 09-Jan-2025 DEFINITION ∆HNH Sp-dCas9 ACCESSION BBa_K2130012 VERSION BBa_K2130012.1 FEATURES Location/Qualifiers misc_feature 2326..2337 /label=GGGS linker ORIGIN 1 atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc 61 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc 121 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa 181 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc 241 taccttcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg 301 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc 361 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag 421 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat 481 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat 541 gtcgataaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg 601 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg 661 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat 721 cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa 781 gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc 841 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt 901 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt 961 atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga 1021 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc 1081 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg 1141 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc 1201 aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac 1261 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt 1321 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc 1381 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa 1441 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa 1501 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt 1561 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg 1621 tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc 1681 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc 1741 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc 1801 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc 1861 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct 1921 catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg 1981 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg 2041 gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac 2101 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt 2161 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat acttcagacc 2221 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt 2281 atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagggtgg tggttctgag 2341 ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat caccaagcac 2401 gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga caaactgatt 2461 cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag aaaggacttt 2521 cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc ctacctgaat 2581 gcagtggtag gcactgcact tatcaaaaaa tatcccaagc ttgaatctga atttgtttac 2641 ggagactata aagtgtacga tgttaggaaa atgatcgcaa agtctgagca ggaaataggc 2701 aaggccaccg ctaagtactt cttttacagc aatattatga attttttcaa gaccgagatt 2761 acactggcca atggagagat tcggaagcga ccacttatcg aaacaaacgg agaaacagga 2821 gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc ggaaggtcct gtccatgccg 2881 caggtgaaca tcgttaaaaa gaccgaagta cagaccggag gcttctccaa ggaaagtatc 2941 ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa aagattggga ccccaagaaa 3001 tacggcggat tcgattctcc tacagtcgct tacagtgtac tggttgtggc caaagtggag 3061 aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac aatcatggag 3121 cgatcaagct tcgaaaaaaa ccccatcgac tttctcgagg cgaaaggata taaagaggtc 3181 aaaaaagacc tcatcattaa gcttcccaag tactctctct ttgagcttga aaacggccgg 3241 aaacgaatgc tcgctagtgc gggcgagctt cagaaaggta acgagctggc actgccctct 3301 aaatacgtta atttcttgta tctggccagc cactatgaaa agctcaaagg gtctcccgaa 3361 gataatgagc agaagcagct gttcgtggaa caacacaaac actaccttga tgagatcatc 3421 gagcaaataa gcgagttctc caaaagagtg atcctcgccg acgctaacct cgataaggtg 3481 ctttctgctt acaataagca cagggataag cccatcaggg agcaggcaga aaacattatc 3541 cacttgttta ctctgaccaa cttgggcgcg cctgctgcct tcaagtactt cgacaccacc 3601 atagacagaa agcggtacac ctctacaaag gaggtcctgg acgccacact gattcatcag 3661 tcaattacgg ggctctatga aacaagaatc gacctctctc agctcggtgg agacagcagg 3721 gctgac //