LOCUS BBa_K2130013 4227 bp DNA linear 27-Nov-2024 DEFINITION SpCas9 ACCESSION BBa_K2130013 VERSION BBa_K2130013.1 FEATURES Location/Qualifiers misc_feature 1..45 /label=V5 epitope misc_feature 49..72 /label=SV40 NLS misc_feature 4195..4227 /label=NLS misc_feature 73..4194 /label=Step. pyogenes Cas9 ORIGIN 1 atgggcaagc ccatccctaa ccccctgttg gggctggaca gcaccgctcc caaaaagaaa 61 aggaaggtgg gcattcacgg cgtgcctgcg gccgacaaaa agtacagcat cggccttgat 121 atcggcacca atagcgtggg ctgggccgtt atcacagacg aatacaaggt acccagcaag 181 aagttcaagg tgctggggaa tacagacagg cactctatca agaaaaacct tatcggggct 241 ctgctgtttg actcaggcga gaccgccgag gccaccaggt tgaagaggac cgcaaggcga 301 aggtacaccc ggaggaagaa caggatctgc tatcttcagg agatcttcag caacgagatg 361 gccaaggtgg acgacagctt cttccacagg ctggaggaga gcttccttgt cgaggaggat 421 aagaagcacg aacgacaccc catcttcggc aacatagtcg acgaggtcgc ttatcacgag 481 aagtacccca ccatctacca cctgcgaaag aaattggtgg atagcaccga taaagccgac 541 ttgcgactta tctacttggc tctggcgcac atgattaagt tcaggggcca cttcctgatc 601 gagggcgacc ttaaccccga caacagtgac gtagacaaat tgttcatcca gcttgtacag 661 acctataacc agctgttcga ggaaaaccct attaacgcca gcggggtgga tgcgaaggcc 721 atacttagcg ccaggctgag caaaagcagg cgcttggaga acctgatagc ccagctgccc 781 ggtgaaaaga agaacggcct cttcggtaat ctgattgccc tgagcctggg cctgaccccc 841 aacttcaaga gcaacttcga cctggcagaa gatgccaagc ttcagttgag taaggacacc 901 tatgacgacg acttggacaa tctgctcgcc caaatcggcg accagtacgc tgacctgttc 961 ctcgccgcca agaacctttc tgacgcaatc ctgcttagcg atatccttag ggtgaacaca 1021 gagatcacca aggcccccct gagcgccagc atgatcaaga ggtacgacga gcaccatcag 1081 gacctgaccc ttctgaaggc cctggtgagg cagcaactgc ccgagaagta caaggagatc 1141 tttttcgacc agagcaagaa cggctacgcc ggctacatcg acggcggagc cagccaagag 1201 gagttctaca agttcatcaa gcccatcctg gagaagatgg atggcaccga ggagctgctg 1261 gtgaagctga acagggaaga tttgctccgg aagcagagga cctttgacaa cggtagcatc 1321 ccccaccaga tccacctggg cgagctgcac gcaatactga ggcgacagga ggatttctac 1381 cccttcctca aggacaatag ggagaaaatc gaaaagattc tgaccttcag gatcccctac 1441 tacgtgggcc ctcttgccag gggcaacagc cgattcgctt ggatgacaag aaagagcgag 1501 gagaccatca ccccctggaa cttcgaggaa gtggtggaca aaggagcaag cgcgcagtct 1561 ttcatcgaac ggatgaccaa tttcgacaaa aacctgccta acgagaaggt gctgcccaag 1621 cacagcctgc tttacgagta cttcaccgtg tacaacgagc tcaccaaggt gaaatatgtg 1681 accgagggca tgcgaaaacc cgctttcctg agcggcgagc agaagaaggc catcgtggac 1741 ctgctgttca agaccaacag gaaggtgacc gtgaagcagc tgaaggagga ctacttcaag 1801 aagatcgagt gctttgatag cgtggaaata agcggcgtgg aggacaggtt caacgccagc 1861 ctgggcacct accacgactt gttgaagata atcaaagaca aggatttcct ggataatgag 1921 gagaacgagg atatactcga ggacatcgtg ctgactttga ccctgtttga ggaccgagag 1981 atgattgaag aaaggctcaa aacctacgcc cacctgttcg acgacaaagt gatgaaacaa 2041 ctgaagagac gaagatacac cggctggggc agactgtcca ggaagctcat caacggcatt 2101 agggacaagc agagcggcaa gaccatcctg gatttcctga agtccgacgg cttcgccaac 2161 cgaaacttca tgcagctgat tcacgatgac agcttgacct tcaaggagga catccagaag 2221 gcccaggtta gcggccaggg cgactccctg cacgaacata ttgcaaacct ggcaggctcc 2281 cctgcgatca agaagggcat acttcagacc gttaaggttg tggacgaatt ggtcaaggtc 2341 atgggcaggc acaagcccga aaacatagtt atagagatgg ccagagagaa ccagaccacc 2401 caaaagggcc agaagaacag ccgggagcgc atgaaaagga tcgaggaggg tatcaaggaa 2461 ctcggaagcc agatcctcaa agagcacccc gtggagaata cccagctcca gaacgagaag 2521 ctgtacctgt actaccttca gaacggcagg gacatgtacg ttgaccagga gttggacatc 2581 aacaggcttt cagactatga cgtggatcac atagtgcccc agagctttct taaagacgat 2641 agcatcgaca acaaggtcct gacccgctcc gacaaaaaca ggggcaaaag cgacaacgtg 2701 ccaagcgaag aggtggttaa aaagatgaag aactactgga ggcaactgct caacgcgaaa 2761 ttgatcaccc agagaaagtt cgataacctg accaaggccg agaggggcgg actctccgaa 2821 cttgacaaag cgggcttcat aaagaggcag ctggtcgaga cccgacagat cacgaagcac 2881 gtggcccaaa tcctcgacag cagaatgaat accaagtacg atgagaatga caaactcatc 2941 agggaagtga aagtgattac cctgaagagc aagttggtgt ccgactttcg caaagatttc 3001 cagttctaca aggtgaggga gatcaacaac taccaccatg cccacgacgc atacctgaac 3061 gccgtggtcg gcaccgccct gattaagaag tatccaaagc tggagtccga atttgtctac 3121 ggcgactaca aagtttacga tgtgaggaag atgatcgcta agagcgaaca ggagatcggc 3181 aaggccaccg ctaagtattt cttctacagc aacatcatga actttttcaa gaccgagatc 3241 acacttgcca acggcgaaat caggaagagg ccgcttatcg agaccaacgg tgagaccggc 3301 gagatcgtgt gggacaaggg cagggacttc gccaccgtga ggaaagtcct gagcatgccc 3361 caggtgaata ttgtgaaaaa aactgaggtg cagacaggcg gctttagcaa ggaatccatc 3421 ctgcccaaga ggaacagcga caagctgatc gcccggaaga aggactggga ccctaagaag 3481 tatggaggct tcgacagccc caccgtagcc tacagcgtgc tggtggtcgc gaaggtagag 3541 aaggggaaga gcaagaaact gaagagcgtg aaggagctgc tcggcataac catcatggag 3601 aggtccagct ttgagaagaa ccccattgac tttttggaag ccaagggcta caaagaggtc 3661 aaaaaggacc tgatcatcaa actccccaag tactccctgt ttgaattgga gaacggcaga 3721 aagaggatgc tggcgagcgc tggggaactg caaaagggca acgaactggc gctgcccagc 3781 aagtacgtga attttctgta cctggcgtcc cactacgaaa agctgaaagg cagccccgag 3841 gacaacgagc agaagcagct gttcgtggag cagcacaagc attacctgga cgagataatc 3901 gagcaaatca gcgagttcag caagagggtg attctggccg acgcgaacct ggataaggtc 3961 ctcagcgcct acaacaagca ccgagacaaa cccatcaggg agcaggccga gaatatcata 4021 cacctgttca ccctgacaaa tctgggcgca cctgcggcat tcaaatactt cgataccacc 4081 atcgacagga aaaggtacac tagcactaag gaggtgctgg atgccacctt gatccaccag 4141 tccattaccg gcctgtatga gaccaggatc gacctgagcc agcttggagg cgactctagg 4201 gcggacccaa aaaagaaaag gaaggtg //