LOCUS BBa_K2130014 3255 bp DNA linear 25-Dec-2024 DEFINITION SaCas9 ACCESSION BBa_K2130014 VERSION BBa_K2130014.1 FEATURES Location/Qualifiers misc_feature 7..30 /label=SV40 NLS misc_feature 3208..3255 /label=NLS ORIGIN 1 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc caagcggaac 61 tacatcctgg gcctggacat cggcatcacc agcgtgggct acggcatcat cgactacgag 121 acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag aggccaacgt ggaaaacaac 181 gagggcaggc ggagcaagag aggcgccaga aggctgaagc ggcggaggcg gcatagaatc 241 cagagagtga agaagctgct gttcgactac aacctgctga ccgaccacag cgagctgagc 301 ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc agaagctgag cgaggaagag 361 ttctctgccg ccctgctgca cctggccaag agaagaggcg tgcacaacgt gaacgaggtg 421 gaagaggaca ccggcaacga gctgtccacc aaagagcaga tcagccggaa cagcaaggcc 481 ctggaagaga aatacgtggc cgaacttcag ctggaacggc tgaagaaaga cggcgaagtg 541 cggggcagca tcaacagatt caagaccagc gactacgtga aagaagccaa acagctgctg 601 aaggtgcaga aggcctacca ccagctggac cagagcttca tcgacaccta catcgacctg 661 ctggaaaccc ggcggaccta ctatgaggga cctggcgagg gcagcccctt cggctggaag 721 gacatcaaag aatggtacga gatgctgatg ggccactgca cctacttccc cgaggaactg 781 cggagcgtga agtacgccta caacgccgac ctgtacaacg ccctgaacga cctgaacaat 841 ctcgtgatca ccagggacga gaacgagaag ctggaatatt acgagaagtt ccagatcatc 901 gagaacgtgt tcaagcagaa gaagaagccc accctgaagc agatcgccaa agaaatcctc 961 gtgaacgaag aggatattaa gggctacaga gtgaccagca ccggcaagcc cgagttcacc 1021 aacctgaagg tgtaccacga catcaaggac attaccgccc ggaaagagat tattgagaac 1081 gccgagctgc tggatcagat tgccaagatc ctgaccatct accagagcag cgaggacatc 1141 caggaagaac tgaccaatct gaactccgag ctgacccagg aagagatcga gcagatctct 1201 aatctgaagg gctataccgg cacccacaac ctgagcctga aggccatcaa cctgatcctg 1261 gacgagctgt ggcacaccaa cgacaaccag atcgctatct tcaaccggct gaagctggtg 1321 cccaagaagg tggacctgtc ccagcagaaa gagatcccca ccaccctggt ggacgacttc 1381 atcctgagcc ccgtcgtgaa gagaagcttc atccagagca tcaaagtgat caacgccatc 1441 atcaagaagt acggcctgcc caacgacatc attatcgagc tggcccgcga gaagaactcc 1501 aaggacgccc agaaaatgat caacgagatg cagaagcgga accggcagac caacgagcgg 1561 atcgaggaaa tcatccggac caccggcaaa gagaacgcca agtacctgat cgagaagatc 1621 aagctgcacg acatgcagga aggcaagtgc ctgtacagcc tggaagccat ccctctggaa 1681 gatctgctga acaacccctt caactatgag gtggaccaca tcatccccag aagcgtgtcc 1741 ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg aagaaaacag caagaagggc 1801 aaccggaccc cattccagta cctgagcagc agcgacagca agatcagcta cgaaaccttc 1861 aagaagcaca tcctgaatct ggccaagggc aagggcagaa tcagcaagac caagaaagag 1921 tatctgctgg aagaacggga catcaacagg ttctccgtgc agaaagactt catcaaccgg 1981 aacctggtgg ataccagata cgccaccaga ggcctgatga acctgctgcg gagctacttc 2041 agagtgaaca acctggacgt gaaagtgaag tccatcaatg gcggcttcac cagctttctg 2101 cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt acaagcacca cgccgaggac 2161 gccctgatca ttgccaacgc cgatttcatc ttcaaagagt ggaagaaact ggacaaggcc 2221 aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg ccgagagcat gcccgagatc 2281 gaaaccgagc aggagtacaa agagatcttc atcacccccc accagatcaa gcacattaag 2341 gacttcaagg actacaagta cagccaccgg gtggacaaga agcctaatag agagctgatt 2401 aacgacaccc tgtactccac ccggaaggac gacaagggca acaccctgat cgtgaacaat 2461 ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa agctgatcaa caagagcccc 2521 gaaaagctgc tgatgtacca ccacgacccc cagacctacc agaaactgaa gctgattatg 2581 gaacagtacg gcgacgagaa gaatcccctg tacaagtact acgaggaaac cgggaactac 2641 ctgaccaagt actccaaaaa ggacaacggc cccgtgatca agaagattaa gtattacggc 2701 aacaaactga acgcccatct ggacatcacc gacgactacc ccaacagcag aaacaaggtc 2761 gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc tggacaatgg cgtgtacaag 2821 ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa actactacga agtgaatagc 2881 aagtgctatg aggaagctaa gaagctgaag aagatcagca accaggccga gtttatcgcc 2941 tccttctaca acaacgatct gatcaagatc aacggcgagc tgtatagagt gatcggcgtg 3001 aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg acatcaccta ccgcgagtac 3061 ctggaaaaca tgaacgacaa gaggcccccc aggatcatta agacaatcgc ctccaagacc 3121 cagagcatta agaagtacag cacagacatt ctgggcaacc tgtatgaagt gaaatctaag 3181 aagcaccctc agatcatcaa aaagggcaaa aggccggcgg ccacgaaaaa ggccggccag 3241 gcaaaaaaga aaaag //