BBa_K2132001
1
BBa_K2132001
CsgASpyCatcherHisTag
2016-08-25T11:00:00Z
2016-08-25T11:53:39Z
CsgA comes from the genomic sequence of the E. Coli. We obtained the sequence of CsgA from the Timothy K. Lu lab.
This is the subunit of the biofilm of E. Coli Curli system linked to SpyCatcher and HisTag. The two added parts can be used for various functions with SpyTag and other His tag-binding materials like quantum dots.
false
false
_2601_
31784
31784
9
false
This part already includes the signal peptide for the secretion of CsgA.
Also, remember, this part has two His tags.
false
Shijie Gu
annotation2481636
1
csgA with signal peptide and HisTag at N-teminal
range2481636
1
1
471
annotation2481637
1
Linker
range2481637
1
472
501
annotation2481639
1
His tag
range2481639
1
847
867
annotation2481638
1
SpyCatcher
range2481638
1
502
846
BBa_K2132001_sequence
1
atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaatcaccatcaccatcaccaccattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagggtggcggtggctctggtggcggtggctctgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattctcgagcaccaccaccaccaccactga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z