LOCUS       BBa_K2132004            2166 bp    DNA     linear       26-Nov-2024
DEFINITION  HydA with SpyCatcher, Histag and TEV site
ACCESSION   BBa_K2132004
VERSION     BBa_K2132004.1  
FEATURES             Location/Qualifiers
     misc_feature    7..24
                     /label=6x Histag
     misc_feature    25..45
                     /label=TEV site
     CDS             46..1791
                     /label=hydA
     misc_feature    1822..2166
                     /label=SpyCatcher
ORIGIN
        1 atggctcacc accaccacca ccacgaaaac ctgtacttcc aaggaatgaa gaccatcatt
       61 ctgaacggta acgaggttca caccgacaaa gatatcacca ttctggagct ggcgcgtgaa
      121 aacaacgtgg acatcccgac cctgtgcttt ctgaaggatt gcggcaactt cggtaaatgc
      181 ggcgtttgca tggttgaggt ggaaggcaag ggtttccgtg cggcgtgcgt ggcgaaagtt
      241 gaggacggta tggttatcaa caccgaaagc gatgaggtga aggaacgtat taagaaacgt
      301 gttagcatgc tgctggacaa acacgaattt aagtgcggcc agtgcagccg tcgtgagaac
      361 tgcgagttcc tgaaactggt gatcaagacc aaagcgaagg cgagcaagcc gttcctgccg
      421 gaggacaaag atgcgctggt tgacaaccgt agcaaggcga tcgttattga tcgtagcaaa
      481 tgcgttctgt gcggtcgttg cgtggcggcg tgcaagcaac acaccagcac ctgtagcatc
      541 cagtttatta agaaagacgg tcaacgtgcg gtgggcaccg ttgacgatgt gtgcctggac
      601 gatagcacct gcctgctgtg cggccagtgc gttatcgcgt gcccggtggc ggcgctgaaa
      661 gagaagagcc acattgagaa agttcaagaa gcgctgaacg atccgaagaa acacgtgatc
      721 gttgcgatgg cgccgagcgt gcgtaccgcg atgggtgaac tgttcaaaat gggctacggc
      781 aaggacgtta ccggtaaact gtataccgcg ctgcgtatgc tgggcttcga caaggtgttt
      841 gatatcaact tcggtgcgga tatgaccatt atggaggaag cgaccgagct gctgggtcgt
      901 gttaaaaaca acggcccgtt cccgatgttt accagctgct gcccggcgtg ggtgcgtctg
      961 gcgcagaact accacccgga actgctggac aacctgagca gcgcgaagag cccgcagcaa
     1021 atctttggta ccgcgagcaa aacctactat ccgagcatca gcggcattgc gccggaggat
     1081 gtttacaccg tgaccattat gccgtgcaac gacaagaaat atgaggcgga tatcccgttc
     1141 atggaaacca acagcctgcg tgacattgat gcgagcctga ccacccgtga actggcgaaa
     1201 atgatcaagg acgcgaaaat taagtttgcg gacctggagg atggtgaagt tgatccggcg
     1261 atgggcacct acagcggtgc gggtgcgatc tttggtgcga ccggtggcgt gatggaggcg
     1321 gcgattcgta gcgcgaagga cttcgcggaa aacaaagagc tggaaaacgt tgattatacc
     1381 gaggtgcgtg gttttaaagg catcaaagag gcggaagtgg agattgcggg caacaagctg
     1441 aacgtggcgg ttatcaacgg tgcgagcaac ttctttgagt ttatgaaaag cggcaagatg
     1501 aacgagaaac agtaccactt catcgaagtg atggcgtgcc cgggtggctg cattaacggt
     1561 ggcggtcaac cgcacgtgaa cgcgctggac cgtgaaaacg ttgattaccg taagctgcgt
     1621 gcgagcgtgc tgtataacca ggacaaaaac gttctgagca agcgtaagag ccacgacaac
     1681 ccggcgatca ttaagatgta cgatagctat ttcggtaaac cgggcgaagg tctggcgcac
     1741 aagctgctgc acgtgaaata taccaaagac aagaacgtta gcaagcacga gggcggtggc
     1801 agcggtggcg gtagcggtgc gatggtggat accctgagcg gcctgagcag cgaacagggt
     1861 caaagcggcg acatgaccat cgaggaagat agcgcgaccc acattaaatt cagcaagcgt
     1921 gacgaggatg gcaaggaact ggcgggtgcg acgatggagc tgcgtgacag cagcggcaaa
     1981 accatcagca cctggattag cgacggtcaa gtgaaggatt tttacctgta tccgggcaaa
     2041 tacaccttcg ttgagaccgc ggcgccggac ggttatgaag tggcgaccgc gatcaccttt
     2101 accgttaacg aacagggcca agtgaccgtt aacggtaaag cgaccaaggg cgatgcgcac
     2161 atttaa
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