BBa_K2132004 1 BBa_K2132004 HydA with SpyCatcher, Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T03:28:55Z Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydA, and SpyCatcher to the C-terminal site of hydA. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2488823 1 TEV site range2488823 1 25 45 annotation2488839 1 SpyCatcher range2488839 1 1822 2166 annotation2488824 1 hydA range2488824 1 46 1791 annotation2488822 1 6x Histag range2488822 1 7 24 BBa_K2132004_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaagaccatcattctgaacggtaacgaggttcacaccgacaaagatatcaccattctggagctggcgcgtgaaaacaacgtggacatcccgaccctgtgctttctgaaggattgcggcaacttcggtaaatgcggcgtttgcatggttgaggtggaaggcaagggtttccgtgcggcgtgcgtggcgaaagttgaggacggtatggttatcaacaccgaaagcgatgaggtgaaggaacgtattaagaaacgtgttagcatgctgctggacaaacacgaatttaagtgcggccagtgcagccgtcgtgagaactgcgagttcctgaaactggtgatcaagaccaaagcgaaggcgagcaagccgttcctgccggaggacaaagatgcgctggttgacaaccgtagcaaggcgatcgttattgatcgtagcaaatgcgttctgtgcggtcgttgcgtggcggcgtgcaagcaacacaccagcacctgtagcatccagtttattaagaaagacggtcaacgtgcggtgggcaccgttgacgatgtgtgcctggacgatagcacctgcctgctgtgcggccagtgcgttatcgcgtgcccggtggcggcgctgaaagagaagagccacattgagaaagttcaagaagcgctgaacgatccgaagaaacacgtgatcgttgcgatggcgccgagcgtgcgtaccgcgatgggtgaactgttcaaaatgggctacggcaaggacgttaccggtaaactgtataccgcgctgcgtatgctgggcttcgacaaggtgtttgatatcaacttcggtgcggatatgaccattatggaggaagcgaccgagctgctgggtcgtgttaaaaacaacggcccgttcccgatgtttaccagctgctgcccggcgtgggtgcgtctggcgcagaactaccacccggaactgctggacaacctgagcagcgcgaagagcccgcagcaaatctttggtaccgcgagcaaaacctactatccgagcatcagcggcattgcgccggaggatgtttacaccgtgaccattatgccgtgcaacgacaagaaatatgaggcggatatcccgttcatggaaaccaacagcctgcgtgacattgatgcgagcctgaccacccgtgaactggcgaaaatgatcaaggacgcgaaaattaagtttgcggacctggaggatggtgaagttgatccggcgatgggcacctacagcggtgcgggtgcgatctttggtgcgaccggtggcgtgatggaggcggcgattcgtagcgcgaaggacttcgcggaaaacaaagagctggaaaacgttgattataccgaggtgcgtggttttaaaggcatcaaagaggcggaagtggagattgcgggcaacaagctgaacgtggcggttatcaacggtgcgagcaacttctttgagtttatgaaaagcggcaagatgaacgagaaacagtaccacttcatcgaagtgatggcgtgcccgggtggctgcattaacggtggcggtcaaccgcacgtgaacgcgctggaccgtgaaaacgttgattaccgtaagctgcgtgcgagcgtgctgtataaccaggacaaaaacgttctgagcaagcgtaagagccacgacaacccggcgatcattaagatgtacgatagctatttcggtaaaccgggcgaaggtctggcgcacaagctgctgcacgtgaaatataccaaagacaagaacgttagcaagcacgagggcggtggcagcggtggcggtagcggtgcgatggtggataccctgagcggcctgagcagcgaacagggtcaaagcggcgacatgaccatcgaggaagatagcgcgacccacattaaattcagcaagcgtgacgaggatggcaaggaactggcgggtgcgacgatggagctgcgtgacagcagcggcaaaaccatcagcacctggattagcgacggtcaagtgaaggatttttacctgtatccgggcaaatacaccttcgttgagaccgcggcgccggacggttatgaagtggcgaccgcgatcacctttaccgttaacgaacagggccaagtgaccgttaacggtaaagcgaccaagggcgatgcgcacatttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z