LOCUS       BBa_K2132005            1857 bp    DNA     linear       05-Dec-2025
DEFINITION  HydA with SpyTag, Histag and TEV site
ACCESSION   BBa_K2132005
VERSION     BBa_K2132005.1  
FEATURES             Location/Qualifiers
     misc_feature    1816..1857
                     /label=SpyTag
     misc_feature    7..24
                     /label=6x Histag
     misc_feature    25..45
                     /label=TEV site
     CDS             46..1791
                     /label=HydA
ORIGIN
        1 atggctcacc accaccacca ccacgaaaac ctgtacttcc aaggaatgaa gaccatcatt
       61 ctgaacggta acgaagttca caccgacaaa gatatcacca ttctggagct ggcgcgtgaa
      121 aacaacgtgg acatcccgac cctgtgcttt ctgaaggatt gcggcaactt cggtaaatgc
      181 ggcgtttgca tggttgaggt ggaaggcaag ggtttccgtg cggcgtgcgt ggcgaaagtt
      241 gaagacggca tggttatcaa caccgaaagc gatgaggtga aggaacgtat taagaaacgt
      301 gttagcatgc tgctggacaa acacgagttt aagtgcggcc agtgcagccg tcgtgagaac
      361 tgcgagttcc tgaaactggt gattaagacc aaagcgaagg cgagcaagcc gttcctgccg
      421 gaggacaaag atgcgctggt tgacaaccgt agcaaggcga tcgttattga tcgtagcaaa
      481 tgcgttctgt gcggccgttg cgtggcggcg tgcaagcaac acaccagcac ctgtagcatc
      541 cagtttatta agaaagacgg tcaacgtgcg gtgggcaccg ttgacgatgt gtgcctggac
      601 gatagcacct gcctgctgtg cggccagtgc gttatcgcgt gcccggtggc ggcgctgaaa
      661 gaaaagagcc acattgagaa agttcaagaa gcgctgaacg atccgaagaa acacgtgatc
      721 gttgcgatgg cgccgagcgt gcgtaccgcg atgggtgaac tgttcaaaat gggctacggc
      781 aaggacgtta ccggtaaact gtataccgcg ctgcgtatgc tgggcttcga caaggtgttt
      841 gatatcaact tcggtgcgga tatgaccatt atggaggaag cgaccgaact gctgggtcgt
      901 gttaaaaaca acggcccgtt cccgatgttt accagctgct gcccggcgtg ggtgcgtctg
      961 gcgcagaact accacccgga gctgctggac aacctgagca gcgcgaagag cccgcagcaa
     1021 atctttggca ccgcgagcaa aacctactat ccgagcatca gcggtattgc gccggaagat
     1081 gtttacaccg tgaccattat gccgtgcaac gacaagaaat atgaggcgga tatcccgttc
     1141 atggaaacca acagcctgcg tgacattgat gcgagcctga ccacccgtga gctggcgaaa
     1201 atgatcaagg acgcgaaaat taagtttgcg gacctggagg atggtgaagt tgatccggcg
     1261 atgggcacct acagcggtgc gggtgcgatc tttggtgcga ccggtggcgt gatggaggcg
     1321 gcgattcgta gcgcgaagga cttcgcggaa aacaaagagc tggaaaacgt tgattatacc
     1381 gaggtgcgtg gttttaaagg catcaaagag gcggaagtgg agattgcggg caacaagctg
     1441 aacgtggcgg ttatcaacgg tgcgagcaac ttctttgaat ttatgaaaag cggcaagatg
     1501 aacgagaaac agtaccactt catcgaagtg atggcgtgcc cgggtggctg cattaacggt
     1561 ggcggtcaac cgcacgtgaa cgcgctggac cgtgagaacg ttgattaccg taagctgcgt
     1621 gcgagcgtgc tgtataacca agacaaaaac gttctgagca agcgtaagag ccacgacaac
     1681 ccggcgatca ttaagatgta cgatagctat ttcggtaaac cgggcgaagg tctggcgcac
     1741 aagctgctgc acgtgaaata caccaaagac aagaacgtta gcaagcacga gggcggtggc
     1801 agcggtggcg gtagcgcgca catcgtgatg gttgatgcgt ataaaccgac caagtaa
//