BBa_K2132006 1 BBa_K2132006 HydE with Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T06:03:15Z The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydE. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2489011 1 TEV site range2489011 1 25 45 annotation2489008 1 6x Histag range2489008 1 7 24 annotation2489017 1 hydE range2489017 1 46 1098 BBa_K2132006_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatggacaacatcattaagctgattaacaaagcggaagtgacccacgacctgaccaaggatgaactggttaccctgctgaaagacgatacccacaacgaggaaatctacaaggcggcggatcgtgtgcgtgagaaatatgtgggcgaggaagttcacctgcgtggcctgatcgagttcagcaacatttgcaagcgtaactgcatgtactgcggtctgcgtcgtgacaacaagaacattaaacgttatcgtctggagccggatgaaatcattcacctggcgaagagcgcgaaaaactacggttatcagaccgtggttctgcaaagcggcgaggacgattactataccgtggaaaagatgaaatacatcgttagcgagattaagaaactgaacatggcgatcaccctgagcattggtgaaaagacctttgaggaatacgaggaatatcgtaaaagcggcgcggaccgttacctgatccgtattgagaccaccgacaaggagctgtatgaaaagctggatccgaaaatgagccacgagaaccgtatcaactgcctgaagaacctgcgtaaactgggttatgaagtgggtagcggttgcctggttggtctgccgaaccagaccatcgagagcctggcggacgatattctgttctttaaagaaatcgacgcggatatgattggtgtgggcccgttcatcccgaacgaggacaccccgctgggcgaggaaaagggtggcgagttctttatgagcgtgaaagttaccgcgctgattcgtctgctgctgccggatatcaacattccggcgaccaccgcgatggagagcctgtacccgaacggtcgtagcatcgcgctgaccagcggtgcgaacgtggttatgccgaacgtgaccgagggcgaatatcgtaagctgtacgcgctgtatccgggtaaaatctgcgttaacgataccccgggccactgccgtcaatgcattagcctgaagatcaacaaaattaaccgtaaagttagcgcgaccaaaggttttcgtaagaaaagctacaaagaaagcatcggctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z