BBa_K2132007
1
BBa_K2132007
HydF with Histag and TEV site
2016-10-08T11:00:00Z
2016-10-09T06:05:34Z
The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824.
This is a modified protein, we added Histag and TEV site to the N-terminal site of hydF.
false
false
_2601_
31668
31668
9
false
We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.
false
YIFAN CHEN
annotation2489018
1
hydF
range2489018
1
46
1281
annotation2489009
1
6x Histag
range2489009
1
7
24
annotation2489014
1
TEV site
range2489014
1
25
45
BBa_K2132007_sequence
1
atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaacgagctgaacagcaccccgaagggtgaacgtctgcacatcgcgctgttcggcaagaccaacgtgggcaaaagcagcgttatcaacgcgctgaccagccaagagattgcgctggttagcaacgttaagggcaccaccaccgacccggtgtacaaagcgatggaactgctgccgctgggtccggttatgctgatcgacaccgcgggcctggacgatattagcgatctgggcgagctgcgtcgtggcaagaccctggaagtgctgagcaaaaccgacgttgcgattctggtgtttgatgttgagagcggtatcaccgaatacgataagaacatttatagcctgctgctggagaagaaaatcccgctgattggcgtgctgaacaagatcgacaagaaagattacaaactggaagactataccagccaattcaaaatcccgattgttccgatcagcgcgctgaacaacaagggtattaacaacctgaaagatgagctgatccgtctggcgccggaaaacgacgataagtttaaaatcgtgggtgacctgctgagcccgggcgatatcgcggtgctggttaccccgattgacaaggcggcgccgaaaggccgtctgattctgccgcagcaacagaccatccgtgacattctggagagcgatgcgatcgcgatggtgaccaaggagttcgaactgcgtgaaaccctggacagcctgcgtaagaaaccgaaaatcgttattaccgatagccaggtgttcctgaaggttgcggcggacaccccgaaagatatcctgatgaccagctttagcattctgatggcgcgtcacaagggtgacctgattgagctggcgcgtggcgcgcgtgcgatcgaagatctgaaggacggtgataaaatcctgattgcggaggcgtgcacccaccaccgtcaaagcgacgatatcggcaaggtgaaaattccgcgttggctgcgtcagaaaaccggtaagaaactggagttcgactttagcagcggcttcagctttccgccgaacatcgaagattacgcgctgattgttcactgcgcgggttgcatgctgaaccgtcgtagcatgctgcaccgtattgaaagcagcgtgaagaaacaaatcccgattgtgaactacggtgttctgatcgcgtatgttcagggcattctgccgcgtgcgctgaaaccgttcccgtatgcggaccgtatctttaaccagagcagccgtaactaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z