BBa_K2132007 1 BBa_K2132007 HydF with Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T06:05:34Z The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydF. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2489018 1 hydF range2489018 1 46 1281 annotation2489009 1 6x Histag range2489009 1 7 24 annotation2489014 1 TEV site range2489014 1 25 45 BBa_K2132007_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaacgagctgaacagcaccccgaagggtgaacgtctgcacatcgcgctgttcggcaagaccaacgtgggcaaaagcagcgttatcaacgcgctgaccagccaagagattgcgctggttagcaacgttaagggcaccaccaccgacccggtgtacaaagcgatggaactgctgccgctgggtccggttatgctgatcgacaccgcgggcctggacgatattagcgatctgggcgagctgcgtcgtggcaagaccctggaagtgctgagcaaaaccgacgttgcgattctggtgtttgatgttgagagcggtatcaccgaatacgataagaacatttatagcctgctgctggagaagaaaatcccgctgattggcgtgctgaacaagatcgacaagaaagattacaaactggaagactataccagccaattcaaaatcccgattgttccgatcagcgcgctgaacaacaagggtattaacaacctgaaagatgagctgatccgtctggcgccggaaaacgacgataagtttaaaatcgtgggtgacctgctgagcccgggcgatatcgcggtgctggttaccccgattgacaaggcggcgccgaaaggccgtctgattctgccgcagcaacagaccatccgtgacattctggagagcgatgcgatcgcgatggtgaccaaggagttcgaactgcgtgaaaccctggacagcctgcgtaagaaaccgaaaatcgttattaccgatagccaggtgttcctgaaggttgcggcggacaccccgaaagatatcctgatgaccagctttagcattctgatggcgcgtcacaagggtgacctgattgagctggcgcgtggcgcgcgtgcgatcgaagatctgaaggacggtgataaaatcctgattgcggaggcgtgcacccaccaccgtcaaagcgacgatatcggcaaggtgaaaattccgcgttggctgcgtcagaaaaccggtaagaaactggagttcgactttagcagcggcttcagctttccgccgaacatcgaagattacgcgctgattgttcactgcgcgggttgcatgctgaaccgtcgtagcatgctgcaccgtattgaaagcagcgtgaagaaacaaatcccgattgtgaactacggtgttctgatcgcgtatgttcagggcattctgccgcgtgcgctgaaaccgttcccgtatgcggaccgtatctttaaccagagcagccgtaactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z