LOCUS BBa_K2132100 5979 bp DNA linear 26-Nov-2024 DEFINITION HydA,E,F,G together with loxP sites ACCESSION BBa_K2132100 VERSION BBa_K2132100.1 FEATURES Location/Qualifiers misc_feature 1816..1857 /label=SpyTag misc_feature 7..24 /label=6x Histag misc_feature 25..45 /label=TEV site CDS 46..1791 /label=HydA CDS 1..1857 /label=BBa_K2132005 misc_feature 1866..1901 /label=BBa_K416002 promoter 1910..1944 /label=BBa_J23110 misc_feature 1957..1974 /label=6x Histag misc_feature 1975..1995 /label=TEV site CDS 1996..3231 /label=hydF CDS 1951..3231 /label=BBa_K2132007 misc_feature 3240..3275 /label=BBa_K416002 promoter 3284..3318 /label=BBa_J23110 misc_feature 3331..3348 /label=6x Histag misc_feature 3349..3369 /label=TEV site CDS 3370..4794 /label=hydG CDS 3325..4788 /label=BBa_K2132008 misc_feature 4797..4832 /label=BBa_K416002 promoter 4841..4875 /label=BBa_J23110 misc_feature 4888..4905 /label=6x Histag misc_feature 4906..4926 /label=TEV site CDS 4927..5979 /label=hydE CDS 4882..5979 /label=BBa_K2132006 ORIGIN 1 atggctcacc accaccacca ccacgaaaac ctgtacttcc aaggaatgaa gaccatcatt 61 ctgaacggta acgaagttca caccgacaaa gatatcacca ttctggagct ggcgcgtgaa 121 aacaacgtgg acatcccgac cctgtgcttt ctgaaggatt gcggcaactt cggtaaatgc 181 ggcgtttgca tggttgaggt ggaaggcaag ggtttccgtg cggcgtgcgt ggcgaaagtt 241 gaagacggca tggttatcaa caccgaaagc gatgaggtga aggaacgtat taagaaacgt 301 gttagcatgc tgctggacaa acacgagttt aagtgcggcc agtgcagccg tcgtgagaac 361 tgcgagttcc tgaaactggt gattaagacc aaagcgaagg cgagcaagcc gttcctgccg 421 gaggacaaag atgcgctggt tgacaaccgt agcaaggcga tcgttattga tcgtagcaaa 481 tgcgttctgt gcggccgttg cgtggcggcg tgcaagcaac acaccagcac ctgtagcatc 541 cagtttatta agaaagacgg tcaacgtgcg gtgggcaccg ttgacgatgt gtgcctggac 601 gatagcacct gcctgctgtg cggccagtgc gttatcgcgt gcccggtggc ggcgctgaaa 661 gaaaagagcc acattgagaa agttcaagaa gcgctgaacg atccgaagaa acacgtgatc 721 gttgcgatgg cgccgagcgt gcgtaccgcg atgggtgaac tgttcaaaat gggctacggc 781 aaggacgtta ccggtaaact gtataccgcg ctgcgtatgc tgggcttcga caaggtgttt 841 gatatcaact tcggtgcgga tatgaccatt atggaggaag cgaccgaact gctgggtcgt 901 gttaaaaaca acggcccgtt cccgatgttt accagctgct gcccggcgtg ggtgcgtctg 961 gcgcagaact accacccgga gctgctggac aacctgagca gcgcgaagag cccgcagcaa 1021 atctttggca ccgcgagcaa aacctactat ccgagcatca gcggtattgc gccggaagat 1081 gtttacaccg tgaccattat gccgtgcaac gacaagaaat atgaggcgga tatcccgttc 1141 atggaaacca acagcctgcg tgacattgat gcgagcctga ccacccgtga gctggcgaaa 1201 atgatcaagg acgcgaaaat taagtttgcg gacctggagg atggtgaagt tgatccggcg 1261 atgggcacct acagcggtgc gggtgcgatc tttggtgcga ccggtggcgt gatggaggcg 1321 gcgattcgta gcgcgaagga cttcgcggaa aacaaagagc tggaaaacgt tgattatacc 1381 gaggtgcgtg gttttaaagg catcaaagag gcggaagtgg agattgcggg caacaagctg 1441 aacgtggcgg ttatcaacgg tgcgagcaac ttctttgaat ttatgaaaag cggcaagatg 1501 aacgagaaac agtaccactt catcgaagtg atggcgtgcc cgggtggctg cattaacggt 1561 ggcggtcaac cgcacgtgaa cgcgctggac cgtgagaacg ttgattaccg taagctgcgt 1621 gcgagcgtgc tgtataacca agacaaaaac gttctgagca agcgtaagag ccacgacaac 1681 ccggcgatca ttaagatgta cgatagctat ttcggtaaac cgggcgaagg tctggcgcac 1741 aagctgctgc acgtgaaata caccaaagac aagaacgtta gcaagcacga gggcggtggc 1801 agcggtggcg gtagcgcgca catcgtgatg gttgatgcgt ataaaccgac caagtaatac 1861 tagagataac ttcgtataat gtatgctata cgaagttatc gtactagagt ttacggctag 1921 ctcagtccta ggtacaatgc tagctactag atggctcacc accaccacca ccacgaaaac 1981 ctgtacttcc aaggaatgaa cgagctgaac agcaccccga agggtgaacg tctgcacatc 2041 gcgctgttcg gcaagaccaa cgtgggcaaa agcagcgtta tcaacgcgct gaccagccaa 2101 gagattgcgc tggttagcaa cgttaagggc accaccaccg acccggtgta caaagcgatg 2161 gaactgctgc cgctgggtcc ggttatgctg atcgacaccg cgggcctgga cgatattagc 2221 gatctgggcg agctgcgtcg tggcaagacc ctggaagtgc tgagcaaaac cgacgttgcg 2281 attctggtgt ttgatgttga gagcggtatc accgaatacg ataagaacat ttatagcctg 2341 ctgctggaga agaaaatccc gctgattggc gtgctgaaca agatcgacaa gaaagattac 2401 aaactggaag actataccag ccaattcaaa atcccgattg ttccgatcag cgcgctgaac 2461 aacaagggta ttaacaacct gaaagatgag ctgatccgtc tggcgccgga aaacgacgat 2521 aagtttaaaa tcgtgggtga cctgctgagc ccgggcgata tcgcggtgct ggttaccccg 2581 attgacaagg cggcgccgaa aggccgtctg attctgccgc agcaacagac catccgtgac 2641 attctggaga gcgatgcgat cgcgatggtg accaaggagt tcgaactgcg tgaaaccctg 2701 gacagcctgc gtaagaaacc gaaaatcgtt attaccgata gccaggtgtt cctgaaggtt 2761 gcggcggaca ccccgaaaga tatcctgatg accagcttta gcattctgat ggcgcgtcac 2821 aagggtgacc tgattgagct ggcgcgtggc gcgcgtgcga tcgaagatct gaaggacggt 2881 gataaaatcc tgattgcgga ggcgtgcacc caccaccgtc aaagcgacga tatcggcaag 2941 gtgaaaattc cgcgttggct gcgtcagaaa accggtaaga aactggagtt cgactttagc 3001 agcggcttca gctttccgcc gaacatcgaa gattacgcgc tgattgttca ctgcgcgggt 3061 tgcatgctga accgtcgtag catgctgcac cgtattgaaa gcagcgtgaa gaaacaaatc 3121 ccgattgtga actacggtgt tctgatcgcg tatgttcagg gcattctgcc gcgtgcgctg 3181 aaaccgttcc cgtatgcgga ccgtatcttt aaccagagca gccgtaacta atactagaga 3241 taacttcgta taatgtatgc tatacgaagt tatcgtacta gagtttacgg ctagctcagt 3301 cctaggtaca atgctagcta ctagatggct caccaccacc accaccacga aaacctgtac 3361 ttccaaggaa tgtacaacgt gaagagcaaa gttgcgaccg agttcatcag cgacgaggaa 3421 atcattgata gcctggaata tgcgaagcag aacaaaagca accgtgagct gattgacagc 3481 atcattgaaa aggcgaaaga gtgcaagggt ctgacccacc gtgatgcggc ggttctgctg 3541 gaatgcgacc tggaggatga aaacgagaag atgtttaaac tggcgcgtga gatcaagcaa 3601 aaattctacg gtaaccgtat tgtgatgttt gcgccgctgt acctgagcaa ctattgcgtg 3661 aacggctgcg tttactgccc gtatcaccac aagaacaaac acatcgcgcg taagaaactg 3721 agccaggaag acgttaagcg tgagaccatt gcgctgcaag atatgggtca caaacgtctg 3781 gcgctggaag cgggcgagga cccggtgaac aacccgatcg agtacattct ggattgcatc 3841 aaaaccattt atagcatcaa gcacaaaaac ggcgcgatcc gtcgtgtgaa cgttaacatt 3901 gcggcgacca ccgttgaaaa ctacaagaaa ctgaaggacg cgggtattgg cacctatatc 3961 ctgttccagg agacctacaa caagaaaagc tatgaggaac tgcacccgac cggtccgaaa 4021 cacgactacg cgtatcacac cgaagcgatg gatcgtgcga tggagggtgg catcgacgat 4081 gtgggtattg gcgttctgtt cggtctgaac atgtacaagt atgattttgt gggcctgctg 4141 atgcacgcgg aacacctgga ggcggcgatg ggtgtgggtc cgcacaccat cagcgttccg 4201 cgtattcgtc cggcggacga tatcgacccg gaaaacttca gcaacgcgat tagcgatgaa 4261 atctttgaga aaattgtggc gatcattcgt atcgcggttc cgtacaccgg tatgattgtg 4321 agcacccgtg agagcaagaa aacccgtgaa cgtgttctgg agctgggcat tagccagatc 4381 agcggtggca gcagcaccag cgtgggtggc tacgttgaaa gcgagccgga ggaagacaac 4441 agcagccaat ttgaagttaa cgacaaccgt accctggatg aaatcgtgaa ctggctgctg 4501 gagatgaact acattccgag cttctgcacc gcgtgctatc gtgagggtcg taccggcgac 4561 cgttttatga gcctggttaa gagcggtcag atcgcgaact gctgccaacc gaacgcgctg 4621 atgaccctga aagaatacct ggaggattat gcgagcagca acacccagaa gaacggcgaa 4681 gcgctgatcg cgagcgaagt ggagaaaatt ccgaacgaga aggtgaaaag catcgttaag 4741 aaacacctga ccgaactgaa agagggtcaa cgtgacttcc gtttttaata ctagagataa 4801 cttcgtataa tgtatgctat acgaagttat cgtactagag tttacggcta gctcagtcct 4861 aggtacaatg ctagctacta gatggctcac caccaccacc accacgaaaa cctgtacttc 4921 caaggaatgg acaacatcat taagctgatt aacaaagcgg aagtgaccca cgacctgacc 4981 aaggatgaac tggttaccct gctgaaagac gatacccaca acgaggaaat ctacaaggcg 5041 gcggatcgtg tgcgtgagaa atatgtgggc gaggaagttc acctgcgtgg cctgatcgag 5101 ttcagcaaca tttgcaagcg taactgcatg tactgcggtc tgcgtcgtga caacaagaac 5161 attaaacgtt atcgtctgga gccggatgaa atcattcacc tggcgaagag cgcgaaaaac 5221 tacggttatc agaccgtggt tctgcaaagc ggcgaggacg attactatac cgtggaaaag 5281 atgaaataca tcgttagcga gattaagaaa ctgaacatgg cgatcaccct gagcattggt 5341 gaaaagacct ttgaggaata cgaggaatat cgtaaaagcg gcgcggaccg ttacctgatc 5401 cgtattgaga ccaccgacaa ggagctgtat gaaaagctgg atccgaaaat gagccacgag 5461 aaccgtatca actgcctgaa gaacctgcgt aaactgggtt atgaagtggg tagcggttgc 5521 ctggttggtc tgccgaacca gaccatcgag agcctggcgg acgatattct gttctttaaa 5581 gaaatcgacg cggatatgat tggtgtgggc ccgttcatcc cgaacgagga caccccgctg 5641 ggcgaggaaa agggtggcga gttctttatg agcgtgaaag ttaccgcgct gattcgtctg 5701 ctgctgccgg atatcaacat tccggcgacc accgcgatgg agagcctgta cccgaacggt 5761 cgtagcatcg cgctgaccag cggtgcgaac gtggttatgc cgaacgtgac cgagggcgaa 5821 tatcgtaagc tgtacgcgct gtatccgggt aaaatctgcg ttaacgatac cccgggccac 5881 tgccgtcaat gcattagcct gaagatcaac aaaattaacc gtaaagttag cgcgaccaaa 5941 ggttttcgta agaaaagcta caaagaaagc atcggctaa //