BBa_K2132006 1 BBa_K2132006 HydE with Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T06:03:15Z The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydE. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2489011 1 TEV site range2489011 1 25 45 annotation2489008 1 6x Histag range2489008 1 7 24 annotation2489017 1 hydE range2489017 1 46 1098 BBa_K2132005 1 BBa_K2132005 HydA with SpyTag, Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T05:45:15Z The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydA, and SpyTag to the C-terminal site of hydA. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2489005 1 6x Histag range2489005 1 7 24 annotation2489007 1 HydA range2489007 1 46 1791 annotation2489006 1 TEV site range2489006 1 25 45 annotation2489004 1 SpyTag range2489004 1 1816 1857 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K2132007 1 BBa_K2132007 HydF with Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T06:05:34Z The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydF. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2489018 1 hydF range2489018 1 46 1281 annotation2489009 1 6x Histag range2489009 1 7 24 annotation2489014 1 TEV site range2489014 1 25 45 BBa_K2132008 1 BBa_K2132008 HydG with Histag and TEV site 2016-10-08T11:00:00Z 2016-10-09T06:51:16Z The sequence of hydA hydrogenase gene is from the chromosome of Clostridium acetobutylicum ATCC 824. This is a modified protein, we added Histag and TEV site to the N-terminal site of hydG. false false _2601_ 31668 31668 9 false We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag. false YIFAN CHEN annotation2489019 1 hydG range2489019 1 46 1470 annotation2489015 1 TEV site range2489015 1 25 45 annotation2489010 1 6x Histag range2489010 1 7 24 BBa_K416002 1 BBa_K416002 36 Base Pair LoxP 2010-05-31T11:00:00Z 2015-05-08T01:12:27Z Amplified and biobricked from E. coli. The antibody for the c-myc transcription factor. This protein binds to Enhancer Box sequences and recruits histone acetyltransferases, serving as a traditional transcription factor and a regulator of global chromatin structure. false false _527_ 0 6462 9 It's complicated true The coding region of the gene was isolated via PCR. Biobrick prefix and suffix were added during amplification, and ligated into Ampicillin resistant plasmids. false Russell Durrett BBa_K2132100 1 BBa_K2132100 HydA,E,F,G together with loxP sites 2016-10-13T11:00:00Z 2016-10-14T09:22:41Z Clostridium acetobutylicum. However, in our project, it is synthesized. It contains all the necessary subunits for FeFe hydrogenase from Clostridium. acetobutylicum. false false _2601_ 31784 31784 9 false There are many loxP sites. false Shijie Gu component2511834 1 BBa_K416002 component2511840 1 BBa_K416002 component2511839 1 BBa_K2132008 component2511829 1 BBa_J23110 component2511841 1 BBa_J23110 component2511827 1 BBa_K2132005 component2511845 1 BBa_K2132006 component2511835 1 BBa_J23110 component2511828 1 BBa_K416002 component2511833 1 BBa_K2132007 annotation2511845 1 BBa_K2132006 range2511845 1 4882 5979 annotation2511835 1 BBa_J23110 range2511835 1 3284 3318 annotation2511841 1 BBa_J23110 range2511841 1 4841 4875 annotation2511840 1 BBa_K416002 range2511840 1 4797 4832 annotation2511839 1 BBa_K2132008 range2511839 1 3325 4788 annotation2511829 1 BBa_J23110 range2511829 1 1910 1944 annotation2511834 1 BBa_K416002 range2511834 1 3240 3275 annotation2511827 1 BBa_K2132005 range2511827 1 1 1857 annotation2511828 1 BBa_K416002 range2511828 1 1866 1901 annotation2511833 1 BBa_K2132007 range2511833 1 1951 3231 BBa_K2132007_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaacgagctgaacagcaccccgaagggtgaacgtctgcacatcgcgctgttcggcaagaccaacgtgggcaaaagcagcgttatcaacgcgctgaccagccaagagattgcgctggttagcaacgttaagggcaccaccaccgacccggtgtacaaagcgatggaactgctgccgctgggtccggttatgctgatcgacaccgcgggcctggacgatattagcgatctgggcgagctgcgtcgtggcaagaccctggaagtgctgagcaaaaccgacgttgcgattctggtgtttgatgttgagagcggtatcaccgaatacgataagaacatttatagcctgctgctggagaagaaaatcccgctgattggcgtgctgaacaagatcgacaagaaagattacaaactggaagactataccagccaattcaaaatcccgattgttccgatcagcgcgctgaacaacaagggtattaacaacctgaaagatgagctgatccgtctggcgccggaaaacgacgataagtttaaaatcgtgggtgacctgctgagcccgggcgatatcgcggtgctggttaccccgattgacaaggcggcgccgaaaggccgtctgattctgccgcagcaacagaccatccgtgacattctggagagcgatgcgatcgcgatggtgaccaaggagttcgaactgcgtgaaaccctggacagcctgcgtaagaaaccgaaaatcgttattaccgatagccaggtgttcctgaaggttgcggcggacaccccgaaagatatcctgatgaccagctttagcattctgatggcgcgtcacaagggtgacctgattgagctggcgcgtggcgcgcgtgcgatcgaagatctgaaggacggtgataaaatcctgattgcggaggcgtgcacccaccaccgtcaaagcgacgatatcggcaaggtgaaaattccgcgttggctgcgtcagaaaaccggtaagaaactggagttcgactttagcagcggcttcagctttccgccgaacatcgaagattacgcgctgattgttcactgcgcgggttgcatgctgaaccgtcgtagcatgctgcaccgtattgaaagcagcgtgaagaaacaaatcccgattgtgaactacggtgttctgatcgcgtatgttcagggcattctgccgcgtgcgctgaaaccgttcccgtatgcggaccgtatctttaaccagagcagccgtaactaa BBa_K2132100_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaagaccatcattctgaacggtaacgaagttcacaccgacaaagatatcaccattctggagctggcgcgtgaaaacaacgtggacatcccgaccctgtgctttctgaaggattgcggcaacttcggtaaatgcggcgtttgcatggttgaggtggaaggcaagggtttccgtgcggcgtgcgtggcgaaagttgaagacggcatggttatcaacaccgaaagcgatgaggtgaaggaacgtattaagaaacgtgttagcatgctgctggacaaacacgagtttaagtgcggccagtgcagccgtcgtgagaactgcgagttcctgaaactggtgattaagaccaaagcgaaggcgagcaagccgttcctgccggaggacaaagatgcgctggttgacaaccgtagcaaggcgatcgttattgatcgtagcaaatgcgttctgtgcggccgttgcgtggcggcgtgcaagcaacacaccagcacctgtagcatccagtttattaagaaagacggtcaacgtgcggtgggcaccgttgacgatgtgtgcctggacgatagcacctgcctgctgtgcggccagtgcgttatcgcgtgcccggtggcggcgctgaaagaaaagagccacattgagaaagttcaagaagcgctgaacgatccgaagaaacacgtgatcgttgcgatggcgccgagcgtgcgtaccgcgatgggtgaactgttcaaaatgggctacggcaaggacgttaccggtaaactgtataccgcgctgcgtatgctgggcttcgacaaggtgtttgatatcaacttcggtgcggatatgaccattatggaggaagcgaccgaactgctgggtcgtgttaaaaacaacggcccgttcccgatgtttaccagctgctgcccggcgtgggtgcgtctggcgcagaactaccacccggagctgctggacaacctgagcagcgcgaagagcccgcagcaaatctttggcaccgcgagcaaaacctactatccgagcatcagcggtattgcgccggaagatgtttacaccgtgaccattatgccgtgcaacgacaagaaatatgaggcggatatcccgttcatggaaaccaacagcctgcgtgacattgatgcgagcctgaccacccgtgagctggcgaaaatgatcaaggacgcgaaaattaagtttgcggacctggaggatggtgaagttgatccggcgatgggcacctacagcggtgcgggtgcgatctttggtgcgaccggtggcgtgatggaggcggcgattcgtagcgcgaaggacttcgcggaaaacaaagagctggaaaacgttgattataccgaggtgcgtggttttaaaggcatcaaagaggcggaagtggagattgcgggcaacaagctgaacgtggcggttatcaacggtgcgagcaacttctttgaatttatgaaaagcggcaagatgaacgagaaacagtaccacttcatcgaagtgatggcgtgcccgggtggctgcattaacggtggcggtcaaccgcacgtgaacgcgctggaccgtgagaacgttgattaccgtaagctgcgtgcgagcgtgctgtataaccaagacaaaaacgttctgagcaagcgtaagagccacgacaacccggcgatcattaagatgtacgatagctatttcggtaaaccgggcgaaggtctggcgcacaagctgctgcacgtgaaatacaccaaagacaagaacgttagcaagcacgagggcggtggcagcggtggcggtagcgcgcacatcgtgatggttgatgcgtataaaccgaccaagtaatactagagataacttcgtataatgtatgctatacgaagttatcgtactagagtttacggctagctcagtcctaggtacaatgctagctactagatggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaacgagctgaacagcaccccgaagggtgaacgtctgcacatcgcgctgttcggcaagaccaacgtgggcaaaagcagcgttatcaacgcgctgaccagccaagagattgcgctggttagcaacgttaagggcaccaccaccgacccggtgtacaaagcgatggaactgctgccgctgggtccggttatgctgatcgacaccgcgggcctggacgatattagcgatctgggcgagctgcgtcgtggcaagaccctggaagtgctgagcaaaaccgacgttgcgattctggtgtttgatgttgagagcggtatcaccgaatacgataagaacatttatagcctgctgctggagaagaaaatcccgctgattggcgtgctgaacaagatcgacaagaaagattacaaactggaagactataccagccaattcaaaatcccgattgttccgatcagcgcgctgaacaacaagggtattaacaacctgaaagatgagctgatccgtctggcgccggaaaacgacgataagtttaaaatcgtgggtgacctgctgagcccgggcgatatcgcggtgctggttaccccgattgacaaggcggcgccgaaaggccgtctgattctgccgcagcaacagaccatccgtgacattctggagagcgatgcgatcgcgatggtgaccaaggagttcgaactgcgtgaaaccctggacagcctgcgtaagaaaccgaaaatcgttattaccgatagccaggtgttcctgaaggttgcggcggacaccccgaaagatatcctgatgaccagctttagcattctgatggcgcgtcacaagggtgacctgattgagctggcgcgtggcgcgcgtgcgatcgaagatctgaaggacggtgataaaatcctgattgcggaggcgtgcacccaccaccgtcaaagcgacgatatcggcaaggtgaaaattccgcgttggctgcgtcagaaaaccggtaagaaactggagttcgactttagcagcggcttcagctttccgccgaacatcgaagattacgcgctgattgttcactgcgcgggttgcatgctgaaccgtcgtagcatgctgcaccgtattgaaagcagcgtgaagaaacaaatcccgattgtgaactacggtgttctgatcgcgtatgttcagggcattctgccgcgtgcgctgaaaccgttcccgtatgcggaccgtatctttaaccagagcagccgtaactaatactagagataacttcgtataatgtatgctatacgaagttatcgtactagagtttacggctagctcagtcctaggtacaatgctagctactagatggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgtacaacgtgaagagcaaagttgcgaccgagttcatcagcgacgaggaaatcattgatagcctggaatatgcgaagcagaacaaaagcaaccgtgagctgattgacagcatcattgaaaaggcgaaagagtgcaagggtctgacccaccgtgatgcggcggttctgctggaatgcgacctggaggatgaaaacgagaagatgtttaaactggcgcgtgagatcaagcaaaaattctacggtaaccgtattgtgatgtttgcgccgctgtacctgagcaactattgcgtgaacggctgcgtttactgcccgtatcaccacaagaacaaacacatcgcgcgtaagaaactgagccaggaagacgttaagcgtgagaccattgcgctgcaagatatgggtcacaaacgtctggcgctggaagcgggcgaggacccggtgaacaacccgatcgagtacattctggattgcatcaaaaccatttatagcatcaagcacaaaaacggcgcgatccgtcgtgtgaacgttaacattgcggcgaccaccgttgaaaactacaagaaactgaaggacgcgggtattggcacctatatcctgttccaggagacctacaacaagaaaagctatgaggaactgcacccgaccggtccgaaacacgactacgcgtatcacaccgaagcgatggatcgtgcgatggagggtggcatcgacgatgtgggtattggcgttctgttcggtctgaacatgtacaagtatgattttgtgggcctgctgatgcacgcggaacacctggaggcggcgatgggtgtgggtccgcacaccatcagcgttccgcgtattcgtccggcggacgatatcgacccggaaaacttcagcaacgcgattagcgatgaaatctttgagaaaattgtggcgatcattcgtatcgcggttccgtacaccggtatgattgtgagcacccgtgagagcaagaaaacccgtgaacgtgttctggagctgggcattagccagatcagcggtggcagcagcaccagcgtgggtggctacgttgaaagcgagccggaggaagacaacagcagccaatttgaagttaacgacaaccgtaccctggatgaaatcgtgaactggctgctggagatgaactacattccgagcttctgcaccgcgtgctatcgtgagggtcgtaccggcgaccgttttatgagcctggttaagagcggtcagatcgcgaactgctgccaaccgaacgcgctgatgaccctgaaagaatacctggaggattatgcgagcagcaacacccagaagaacggcgaagcgctgatcgcgagcgaagtggagaaaattccgaacgagaaggtgaaaagcatcgttaagaaacacctgaccgaactgaaagagggtcaacgtgacttccgtttttaatactagagataacttcgtataatgtatgctatacgaagttatcgtactagagtttacggctagctcagtcctaggtacaatgctagctactagatggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatggacaacatcattaagctgattaacaaagcggaagtgacccacgacctgaccaaggatgaactggttaccctgctgaaagacgatacccacaacgaggaaatctacaaggcggcggatcgtgtgcgtgagaaatatgtgggcgaggaagttcacctgcgtggcctgatcgagttcagcaacatttgcaagcgtaactgcatgtactgcggtctgcgtcgtgacaacaagaacattaaacgttatcgtctggagccggatgaaatcattcacctggcgaagagcgcgaaaaactacggttatcagaccgtggttctgcaaagcggcgaggacgattactataccgtggaaaagatgaaatacatcgttagcgagattaagaaactgaacatggcgatcaccctgagcattggtgaaaagacctttgaggaatacgaggaatatcgtaaaagcggcgcggaccgttacctgatccgtattgagaccaccgacaaggagctgtatgaaaagctggatccgaaaatgagccacgagaaccgtatcaactgcctgaagaacctgcgtaaactgggttatgaagtgggtagcggttgcctggttggtctgccgaaccagaccatcgagagcctggcggacgatattctgttctttaaagaaatcgacgcggatatgattggtgtgggcccgttcatcccgaacgaggacaccccgctgggcgaggaaaagggtggcgagttctttatgagcgtgaaagttaccgcgctgattcgtctgctgctgccggatatcaacattccggcgaccaccgcgatggagagcctgtacccgaacggtcgtagcatcgcgctgaccagcggtgcgaacgtggttatgccgaacgtgaccgagggcgaatatcgtaagctgtacgcgctgtatccgggtaaaatctgcgttaacgataccccgggccactgccgtcaatgcattagcctgaagatcaacaaaattaaccgtaaagttagcgcgaccaaaggttttcgtaagaaaagctacaaagaaagcatcggctaa BBa_K416002_sequence 1 ataacttcgtataatgtatgctatacgaagttatcg BBa_K2132008_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgtacaacgtgaagagcaaagttgcgaccgagttcatcagcgacgaggaaatcattgatagcctggaatatgcgaagcagaacaaaagcaaccgtgagctgattgacagcatcattgaaaaggcgaaagagtgcaagggtctgacccaccgtgatgcggcggttctgctggaatgcgacctggaggatgaaaacgagaagatgtttaaactggcgcgtgagatcaagcaaaaattctacggtaaccgtattgtgatgtttgcgccgctgtacctgagcaactattgcgtgaacggctgcgtttactgcccgtatcaccacaagaacaaacacatcgcgcgtaagaaactgagccaggaagacgttaagcgtgagaccattgcgctgcaagatatgggtcacaaacgtctggcgctggaagcgggcgaggacccggtgaacaacccgatcgagtacattctggattgcatcaaaaccatttatagcatcaagcacaaaaacggcgcgatccgtcgtgtgaacgttaacattgcggcgaccaccgttgaaaactacaagaaactgaaggacgcgggtattggcacctatatcctgttccaggagacctacaacaagaaaagctatgaggaactgcacccgaccggtccgaaacacgactacgcgtatcacaccgaagcgatggatcgtgcgatggagggtggcatcgacgatgtgggtattggcgttctgttcggtctgaacatgtacaagtatgattttgtgggcctgctgatgcacgcggaacacctggaggcggcgatgggtgtgggtccgcacaccatcagcgttccgcgtattcgtccggcggacgatatcgacccggaaaacttcagcaacgcgattagcgatgaaatctttgagaaaattgtggcgatcattcgtatcgcggttccgtacaccggtatgattgtgagcacccgtgagagcaagaaaacccgtgaacgtgttctggagctgggcattagccagatcagcggtggcagcagcaccagcgtgggtggctacgttgaaagcgagccggaggaagacaacagcagccaatttgaagttaacgacaaccgtaccctggatgaaatcgtgaactggctgctggagatgaactacattccgagcttctgcaccgcgtgctatcgtgagggtcgtaccggcgaccgttttatgagcctggttaagagcggtcagatcgcgaactgctgccaaccgaacgcgctgatgaccctgaaagaatacctggaggattatgcgagcagcaacacccagaagaacggcgaagcgctgatcgcgagcgaagtggagaaaattccgaacgagaaggtgaaaagcatcgttaagaaacacctgaccgaactgaaagagggtcaacgtgacttccgtttttaa BBa_K2132005_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatgaagaccatcattctgaacggtaacgaagttcacaccgacaaagatatcaccattctggagctggcgcgtgaaaacaacgtggacatcccgaccctgtgctttctgaaggattgcggcaacttcggtaaatgcggcgtttgcatggttgaggtggaaggcaagggtttccgtgcggcgtgcgtggcgaaagttgaagacggcatggttatcaacaccgaaagcgatgaggtgaaggaacgtattaagaaacgtgttagcatgctgctggacaaacacgagtttaagtgcggccagtgcagccgtcgtgagaactgcgagttcctgaaactggtgattaagaccaaagcgaaggcgagcaagccgttcctgccggaggacaaagatgcgctggttgacaaccgtagcaaggcgatcgttattgatcgtagcaaatgcgttctgtgcggccgttgcgtggcggcgtgcaagcaacacaccagcacctgtagcatccagtttattaagaaagacggtcaacgtgcggtgggcaccgttgacgatgtgtgcctggacgatagcacctgcctgctgtgcggccagtgcgttatcgcgtgcccggtggcggcgctgaaagaaaagagccacattgagaaagttcaagaagcgctgaacgatccgaagaaacacgtgatcgttgcgatggcgccgagcgtgcgtaccgcgatgggtgaactgttcaaaatgggctacggcaaggacgttaccggtaaactgtataccgcgctgcgtatgctgggcttcgacaaggtgtttgatatcaacttcggtgcggatatgaccattatggaggaagcgaccgaactgctgggtcgtgttaaaaacaacggcccgttcccgatgtttaccagctgctgcccggcgtgggtgcgtctggcgcagaactaccacccggagctgctggacaacctgagcagcgcgaagagcccgcagcaaatctttggcaccgcgagcaaaacctactatccgagcatcagcggtattgcgccggaagatgtttacaccgtgaccattatgccgtgcaacgacaagaaatatgaggcggatatcccgttcatggaaaccaacagcctgcgtgacattgatgcgagcctgaccacccgtgagctggcgaaaatgatcaaggacgcgaaaattaagtttgcggacctggaggatggtgaagttgatccggcgatgggcacctacagcggtgcgggtgcgatctttggtgcgaccggtggcgtgatggaggcggcgattcgtagcgcgaaggacttcgcggaaaacaaagagctggaaaacgttgattataccgaggtgcgtggttttaaaggcatcaaagaggcggaagtggagattgcgggcaacaagctgaacgtggcggttatcaacggtgcgagcaacttctttgaatttatgaaaagcggcaagatgaacgagaaacagtaccacttcatcgaagtgatggcgtgcccgggtggctgcattaacggtggcggtcaaccgcacgtgaacgcgctggaccgtgagaacgttgattaccgtaagctgcgtgcgagcgtgctgtataaccaagacaaaaacgttctgagcaagcgtaagagccacgacaacccggcgatcattaagatgtacgatagctatttcggtaaaccgggcgaaggtctggcgcacaagctgctgcacgtgaaatacaccaaagacaagaacgttagcaagcacgagggcggtggcagcggtggcggtagcgcgcacatcgtgatggttgatgcgtataaaccgaccaagtaa BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_K2132006_sequence 1 atggctcaccaccaccaccaccacgaaaacctgtacttccaaggaatggacaacatcattaagctgattaacaaagcggaagtgacccacgacctgaccaaggatgaactggttaccctgctgaaagacgatacccacaacgaggaaatctacaaggcggcggatcgtgtgcgtgagaaatatgtgggcgaggaagttcacctgcgtggcctgatcgagttcagcaacatttgcaagcgtaactgcatgtactgcggtctgcgtcgtgacaacaagaacattaaacgttatcgtctggagccggatgaaatcattcacctggcgaagagcgcgaaaaactacggttatcagaccgtggttctgcaaagcggcgaggacgattactataccgtggaaaagatgaaatacatcgttagcgagattaagaaactgaacatggcgatcaccctgagcattggtgaaaagacctttgaggaatacgaggaatatcgtaaaagcggcgcggaccgttacctgatccgtattgagaccaccgacaaggagctgtatgaaaagctggatccgaaaatgagccacgagaaccgtatcaactgcctgaagaacctgcgtaaactgggttatgaagtgggtagcggttgcctggttggtctgccgaaccagaccatcgagagcctggcggacgatattctgttctttaaagaaatcgacgcggatatgattggtgtgggcccgttcatcccgaacgaggacaccccgctgggcgaggaaaagggtggcgagttctttatgagcgtgaaagttaccgcgctgattcgtctgctgctgccggatatcaacattccggcgaccaccgcgatggagagcctgtacccgaacggtcgtagcatcgcgctgaccagcggtgcgaacgtggttatgccgaacgtgaccgagggcgaatatcgtaagctgtacgcgctgtatccgggtaaaatctgcgttaacgataccccgggccactgccgtcaatgcattagcctgaagatcaacaaaattaaccgtaaagttagcgcgaccaaaggttttcgtaagaaaagctacaaagaaagcatcggctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z