BBa_K2135003 1 pTet-petF petF on pTet inducible promoter 2016-09-27T11:00:00Z 2016-10-14T11:29:04Z petF comes from Synocosystis and TetR-pTet comes from plasmid from Qi et al, 2013. See respective part's page for more info. pTet, which encodes for ferredoxin, is put on a TetR-pTet inducible promoter. Various concentrations of aTc will result in respective amount of petF transcription. false false _2604_ 29300 29300 9 false No false Harley Greene component2485100 1 BBa_K2135002 component2485103 1 BBa_K2135001 annotation2485100 1 BBa_K2135002 range2485100 1 1 703 annotation2485103 1 BBa_K2135001 range2485103 1 712 1040 BBa_K2135002 1 TetR-pTet TetR-pTet Inducible Promoter 2016-09-26T11:00:00Z 2016-09-27T11:02:48Z Cut from plasmid found in Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression [Qi et al. , 2013] Plasmid #44249 from AddGene pTet is a constitutive promoter that is repressed by TetR. TetR is removed when aTc is added at various concentrations. This part had TetR included and constitutively produced. Therefore, this part can be used as an inducible promoter, which will vary promoter strength depending on the concentration of aTc added. false false _2604_ 29300 29300 9 true No false Harley Greene annotation2485096 1 TetR range2485096 1 1 629 annotation2485097 1 pTet range2485097 1 629 700 BBa_K2135001 1 petF petF 2016-09-26T11:00:00Z 2016-10-14T11:29:36Z Synechocystis Pcc6803 petF codes for ferredoxin, an important electron donor in Synechocystis that is mainly works to be the final electron acceptor in the electron transport chain during photosynthesis. false false _2604_ 29300 29300 9 false No considerations made false Harley Greene annotation2485095 1 native RBS range2485095 1 1 36 annotation2485094 1 petF range2485094 1 37 329 BBa_K2135003_sequence 1 ttaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctggacatcattaattcctaatttttgttgacactctatcgttgatagagttattttaccactccctatcagtgatagagaaaagaatactagagtctctctactgtttttttgagaagaggaaacatctatggcatcctataccgttaaattgatcacccccgatggtgaaagttccatcgaatgctctgacgatacctatatcctcgatgctgcggaagaagctggcctagacctgccctattcctgccgtgctggggcttgctccacctgtgccggtaagatcaccgctggtagtgttgaccaatccgatcagtctttcttggatgatgaccaaattgaagctggttatgttttgacctgtgtagcttatcccacctccgattgcaccattgaaacccacaaagaagaagacctctactaa BBa_K2135001_sequence 1 tctctctactgtttttttgagaagaggaaacatctatggcatcctataccgttaaattgatcacccccgatggtgaaagttccatcgaatgctctgacgatacctatatcctcgatgctgcggaagaagctggcctagacctgccctattcctgccgtgctggggcttgctccacctgtgccggtaagatcaccgctggtagtgttgaccaatccgatcagtctttcttggatgatgaccaaattgaagctggttatgttttgacctgtgtagcttatcccacctccgattgcaccattgaaacccacaaagaagaagacctctactaa BBa_K2135002_sequence 1 ttaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaataagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactatcagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagtaaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggctaattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgcgatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttctaaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgcttattttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaaccttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctggacatcattaattcctaatttttgttgacactctatcgttgatagagttattttaccactccctatcagtgatagagaaaagaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z