LOCUS       BBa_K2136010            1545 bp    DNA     linear       23-Dec-2024
DEFINITION  5' cassete for Chlamydomonas transgenic expression
ACCESSION   BBa_K2136010
VERSION     BBa_K2136010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..275
                     /label=HSP70A enhancer
     misc_feature    294..474
                     /label=rbcs2 promoter
     misc_feature    475..509
                     /label=5' UTR of rbsc2
     promoter        1..509
                     /label=BBa_K2136013
     misc_feature    510..517
                     /label=exon1
     CDS             510..1388
                     /label=BBa_K2136014
     misc_feature    1389..1454
                     /label=BBa_K2136017
     misc_feature    1533..1545
                     /label=scar with XhoI and ClaI sites
     misc_feature    1455..1532
                     /label=F2A peptide
     misc_feature    1455..1545
                     /label=BBa_K2136018
ORIGIN
        1 gacggcgggg agctcgctga ggcttgacat gattggtgcg tatgtttgta tgaagctaca
       61 ggactgattt ggcgggctat gagggcgggg gaagctctgg aagggccgcg atggggcgcg
      121 cggcgtccag aaggcgccat acggcccgct ggcggcaccc atccggtata aaagcccgcg
      181 accccgaacg gtgacctcca ctttcagcga caaacgagca cttatacata cgcgactatt
      241 ctgccgctat acataaccac tcagctagct taagatccca tcaagcttgc atgccgggcg
      301 cgccagaagg agcgcagcca aaccaggatg atgtttgatg gggtatttga gcacttgcaa
      361 cccttatccg gaagccccct ggcccacaaa ggctaggcgc caatgcaagc agttcgcatg
      421 cagcccctgg agcggtgccc tcctgataaa ccggccaggg ggcctatgtt ctttactttt
      481 ttacaagaga agtcactcaa catcttaaaa tggccaggtg agtcgacgag caagcccggc
      541 ggatcaggca gcgtgcttgc agatttgact tgcaacgccc gcattgtgtc gacgaaggct
      601 tttggctcct ctgtcgctgt ctcaagcagc atctaaccct gcgtcgccgt ttccatttgc
      661 aggatggcca tgcatatggc caagctgacc agcgccgttc cggtgctcac cgcgcgcgac
      721 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
      781 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
      841 caggtgagct tgcggggttg cgagcaacac tccagcaacg aacagtgccc aagtcaggaa
      901 tctccagtca gcctgggctt tcggcggctt tttcttgggc aaacagcttg cactcatgcc
      961 agcgcggctt gtccagcctc acttgagctt tccagctgct accagccggg ctatacgaca
     1021 gcgacagagc catagcgtgg aatcacttat ttgggttgcc gaagtagcgg tcggagcgtg
     1081 agttcttggt caagccgccc cttatccggt tcctgtccgt gtctttgtcc ctcgttcacc
     1141 cttcgcggca cccttcatcc ccttgcttgc aggaccaggt ggtgccggac aacaccctgg
     1201 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     1261 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     1321 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     1381 acgccccggt gaagcagacc ctgaacttcg acctgctgaa gctggcgggc gacgtggaga
     1441 gcaacccggg ccccatgcat gcacgcaaga tgggtgccct cgcggtgctc gccgtcgctt
     1501 gcctcgcggc agtggcatcg gttgcgcatg cgatcgatct cgagt
//