BBa_K2136013 1 BBa_K2136013 hsp70 + rbsc2 synthetic constitutive promoter for Chlamydomonas 2016-10-12T11:00:00Z 2016-10-16T05:38:35Z Chlamydomonas reinhardtii AR1-Rbcs2 false false _2606_ 31704 31234 9 false no comments false Livia Seno Ferreira Camargo annotation2513562 1 HSP70A enhancer range2513562 1 1 275 annotation2513563 1 rbcs2 promoter range2513563 1 294 474 annotation2513568 1 5' UTR of rbsc2 range2513568 1 475 509 BBa_K2136014 1 BBa_K2136014 Bleomycin resistance gene 2016-10-12T11:00:00Z 2016-10-16T04:19:21Z The sequence comes from Streptoalloteichus hindustanus. Resistance gene for algae expression. Bleomycin gives a resistance against zeocin, because ble binds to zeocin blocking its funtion. false false _2606_ 31704 31234 9 false To this sequence was added an intron RBCS and a signal peptide 2A false Livia Seno Ferreira Camargo annotation2513455 1 exon1 range2513455 1 1 8 BBa_K2136018 1 BBa_K2136018 Ars2 signal peptide for secretion in Chlamydomonas 2016-10-15T11:00:00Z 2016-10-16T05:43:20Z It comes from the gene sequence for arylsuflatase 2 (Ars2) gene in Chlamydomonas reinhardtii. This sequence is part of the arylsuflatase 2 (Ars2) gene in Chlamydomonas reinhardtii. It is responsible for allowing the secretion of the fused protein to the culture medium, thus allowing cell-lysys independent purification of proteins and/or secretion for other aplications false false _2606_ 0 31704 31704 9 Not in stock false Nothing to add. false Tiago Lubiana Alves annotation2513574 1 scar with XhoI and ClaI sites range2513574 1 79 91 annotation2513575 1 F2A peptide range2513575 1 1 78 BBa_K2136010 1 BBa_K2136010 5' cassete for Chlamydomonas transgenic expression 2016-10-12T11:00:00Z 2016-10-17T08:51:56Z AR1 (hsp70A/rbcs2) + ble 2A Synthethic cassette for algae expression. false false _2606_ 0 31704 31234 9 It's complicated true ??ntrons em ble para aumento de express??o false Livia Seno Ferreira Camargo component2513592 1 BBa_K2136018 component2513589 1 BBa_K2136017 component2513588 1 BBa_K2136014 component2513586 1 BBa_K2136013 annotation2513589 1 BBa_K2136017 range2513589 1 1389 1454 annotation2513588 1 BBa_K2136014 range2513588 1 510 1388 annotation2513586 1 BBa_K2136013 range2513586 1 1 509 annotation2513592 1 BBa_K2136018 range2513592 1 1455 1545 BBa_K2136017 1 BBa_K2136017 F2A self-cleaving peptide sequence 2016-10-15T11:00:00Z 2016-10-16T04:57:34Z foot-and-mouth disease virus The foot-and-mouth disease virus (FMDV) self cleaving peptide sequence (VKQTLNFDLLKLAGDVESNPGP) is capable of undergoing selfcleavage in vitro, thus separating 2 different in-frame peptide sequences. (1) Another peptide of the family, P2A, was more deeply characterized by iGEM14_Warwick (http://parts.igem.org/Part:BBa_K1442039#) References: Ryan M, King A, Thomas G. J. Gen. Virol. 72(11):2727-2732 doi:10.1099/0022-1317-72-11-2727 false false _2606_ 0 31704 31704 9 Not in stock false Nothing to add. false Tiago Lubiana Alves BBa_K2136018_sequence 1 atgcatgcacgcaagatgggtgccctcgcggtgctcgccgtcgcttgcctcgcggcagtggcatcggttgcgcatgcgatcgatctcgagt BBa_K2136014_sequence 1 atggccaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcaggatggccatgcatatggccaagctgaccagcgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtgagcttgcggggttgcgagcaacactccagcaacgaacagtgcccaagtcaggaatctccagtcagcctgggctttcggcggctttttcttgggcaaacagcttgcactcatgccagcgcggcttgtccagcctcacttgagctttccagctgctaccagccgggctatacgacagcgacagagccatagcgtggaatcacttatttgggttgccgaagtagcggtcggagcgtgagttcttggtcaagccgccccttatccggttcctgtccgtgtctttgtccctcgttcacccttcgcggcacccttcatccccttgcttgcaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggacgccccg BBa_K2136013_sequence 1 gacggcggggagctcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaa BBa_K2136017_sequence 1 gtgaagcagaccctgaacttcgacctgctgaagctggcgggcgacgtggagagcaacccgggcccc BBa_K2136010_sequence 1 gacggcggggagctcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaaatggccaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcaggatggccatgcatatggccaagctgaccagcgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtgagcttgcggggttgcgagcaacactccagcaacgaacagtgcccaagtcaggaatctccagtcagcctgggctttcggcggctttttcttgggcaaacagcttgcactcatgccagcgcggcttgtccagcctcacttgagctttccagctgctaccagccgggctatacgacagcgacagagccatagcgtggaatcacttatttgggttgccgaagtagcggtcggagcgtgagttcttggtcaagccgccccttatccggttcctgtccgtgtctttgtccctcgttcacccttcgcggcacccttcatccccttgcttgcaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagcaggacgccccggtgaagcagaccctgaacttcgacctgctgaagctggcgggcgacgtggagagcaacccgggccccatgcatgcacgcaagatgggtgccctcgcggtgctcgccgtcgcttgcctcgcggcagtggcatcggttgcgcatgcgatcgatctcgagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z