BBa_K2139001 1 BBa_K2139001 Coding Sequence of Endo5a 2016-10-12T11:00:00Z 2016-10-30T03:50:32Z Paenibacillus strain ICGEB2008 BBa_K2139000 encompass the coding region for a endo-beta-1,4-glucanase known as Endo5A. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid) ,and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and as such as codon optimized to contain a high GC content. Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154 false false _2609_ 33491 34108 9 false It is codon optimized for Caulobacter cresentus, exhibiting a high GC content. false UBC iGEM 2016 BBa_K2139001_sequence 1 atgaagaagaagggcctgaagaagaccttctttgtcatcgcgagcctcgtcatggggctgaccctctacggctataccccgccgtccgcggacgccgccagcgtcaaggggtattaccacacccagggcaacaagatcgtcgatgagagcggcaaggaggcggccttcaatgggctcaattggttcggcctggaaacccccaattataccctgcatgggctgtggtcgcggtcgatggatgacatgctcgaccaagtcaagaaggaggggtacaatctcatccggctgccctacagcaatcagctcttcgattcgagctcgcgcgcggattccatcgattactataagaacccggatctggtcggcctgacgccgatccagatcatggacaagctgatcgagaaggccggccagcgtggcatccaaatcatcctggatcgccatcgccccgggtccgggggccagtcggagctgtggtatacctcgcagtacccggaatcccggtggatctccgattggaagatgctcgccgaacgttacaagaataacccgaccgtgatcggggcggacctgcacaacgagccccacggccaagccagctgggggaccggggatgtgagcaccgactggcgtctggccgcgcaacgtgcgggcaacgcgatcctctccgtgaacccgaattggctgatcctggtggaaggcgtcgaccacaatgtccagggcaacaattcccagtattggtggggcggcaacctgaccggcgtggccaactaccccgtcgtcctggatgtccccaaccgggtcgtctattccccgcatgattacgggccgggggtgtcgagccaaccctggttcaacgactcgacgtttccgagcaatctccccgccatctgggaccaaacctggggctatatctccaagcaaaacatcgcgccggtgctcgtgggcgagtttggcgggcgcaacgtggatagctcgagcccggaaggcaagtggcaaaatgcgctcgtggactacatcggggcgaacaacctctattttacctactggtccctcaaccccaattcgggcgataccggcggcctgctcctcgacgattgggtgacgtggaatcgcccgaagcaagacatgctgagccggatcatgaagccggtgaactttatcgcggaacaagcgaaggcctccgcggagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z