BBa_K2139003 1 BBa_K2139003 Coding Sequence of E1 2016-10-12T11:00:00Z 2016-10-30T03:51:38Z Acidothermus cellulolyticus BBa_K2139003 encompass the coding region for an endo-beta-1,4-glucanase known as E1. E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli. Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full false false _2609_ 33491 34108 9 false Codon optimized for E. coli false UBC iGEM 2016 BBa_K2139003_sequence 1 atgcctcgcgctctgcgtcgtgtaccaggcagcagagtaatgttgcgcgttggtgtggtagttgctgttttagcgttggttgcggctttggccaatctggcagtcccacgccctgctcgggccgcaggcgggggttattggcatacgagtggccgtgaaatactggacgcaaataatgtgcccgtccgtatcgctgggatcaactggtttgggttcgaaacgtgcaactacgttgttcatggcttgtggagccgcgattatcgcagcatgttagatcagatcaagtccttgggatataatactatacgtcttccctactccgatgatattctgaaacccgggacgatgccaaattctatcaacttttaccagatgaaccaagacctgcaagggttgacctccttgcaagtcatggataagatcgtagcatacgcgggccagatcggacttcgtataatcttagatcgccatagacccgattgtagcggtcagtctgccttgtggtacacaagttcagtatcagaagcaacctggatatctgatttacaagcgcttgcacagcggtataagggaaaccctaccgtcgtgggattcgacttacacaatgagcctcatgacccggcatgttggggctgtggtgacccgtccattgattggcgtttggcagcggaaagagctgggaacgctgtcttatcagtgaatcccaacttgcttatcttcgttgaaggcgttcagtcatacaacggggacagctattggtggggcggcaacctgcaaggcgctggccagtaccccgtagtacttaacgtgccgaacagactggtttattccgcccatgattatgcaacgagcgtctatccacaaacgtggtttagtgaccctacgttcccgaacaatatgccaggtatttggaacaaaaattggggctatctttttaatcagaatatagcgccggtttggttaggtgagtttgggacaacgcttcagtcaaccaccgatcaaacttggctgaaaacattagtacaatatttgcggccgacagcgcagtatggtgcagacagtttccagtggactttctggagttggaatccggactcaggggatactggtgggatcttgaaggacgattggcaaacggtagataccgtgaaagatggttaccttgctccgataaaatcatcgatatttgacccggttggtgcaagcgcaagcccttcctctcaaccctcaccaagtgtatcgccgtcaccgtctccctccccctcagcctctcgcacaccgacgccgactcccactcccactgcgagccccacacccacattgactcccacagcgaccccaactcccacctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z