BBa_K2139006 1 BBa_K2139006 Ptac RBS Gluc1C 2016-10-13T11:00:00Z 2016-10-30T03:57:55Z Paenibacillus sp. MTCC 5639 Gluc1C is a monomeric enyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter cresentus and is codon optimized to contain a high GC content. Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154 false false _2609_ 33491 34108 9 false Codon optimized for Caulobacter cresentus (high GC content). false UBC iGEM 2016 component2527395 1 BBa_K2139002 component2527394 1 BBa_K1813037 annotation2527395 1 BBa_K2139002 range2527395 1 88 1434 annotation2527394 1 BBa_K1813037 range2527394 1 1 81 BBa_K2139002 1 BBa_K2139002 Coding Sequence of Gluc1C 2016-10-12T11:00:00Z 2016-10-30T03:51:13Z Paenibacillus sp. MTCC 5639 BBa_K2139000 encompass the coding region for an 1,4-beta-glucosidase gene known as Gluc1C. Gluc1C is a monomeric enyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter cresentus and is codon optimized to contain a high GC content. Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154 false false _2609_ 33491 34108 9 false Codon optimized for Caulobacter cresentus (high GC content) false UBC iGEM 2016 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1813037 1 BBa_K1813037 ptac and RBS 2015-09-10T11:00:00Z 2015-09-18T07:18:03Z Registry ptac RBS false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 component2449459 1 BBa_B0034 component2449457 1 BBa_K864400 annotation2449457 1 BBa_K864400 range2449457 1 1 61 annotation2449459 1 BBa_B0034 range2449459 1 70 81 BBa_K864400 1 BBa_K864400 Ptac, trp & lac regulated promoter 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG Released HQ 2013 The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]] [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25 false false _1124_ 0 9827 9 In stock false Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone. false Erik Lundin annotation2197238 1 -10 range2197238 1 29 33 annotation2197237 1 -35 range2197237 1 7 12 annotation2197236 1 Ptac range2197236 1 1 40 annotation2197240 1 lacO1 site range2197240 1 36 61 BBa_B0034_sequence 1 aaagaggagaaa BBa_K2139002_sequence 1 atgagcgagaacacgttcatctttccggcgaccttcatgtgggggacgtcgacgtcctcgtaccagatcgagggcgggacggacgaaggcgggcgcacccccagcatctgggatacgttttgtcaaatccccggcaaggtcatcgggggcgattgcggcgatgtcgcctgcgatcacttccaccactttaaggaggacgtgcagctgatgaagcagctcggctttctgcattaccgtttcagcgtggcctggccgcgcatcatgccggccccgggcatcatcaacgaggaaggcctcctgttctatgagcatctgctcgacgaaatcgaactcgccgggctcatccccatgctcaccctctatcactgggatctcccgcagtggatcgaggatgagggggggtggacgcagcgtgagacgatccagcacttcaagacctacgcgagcgtgatcatggaccgtttcggggaacgcatcaactggtggaacacgatcaacgaaccgtattgcgcctccatcctcgggtatgggacgggggaacatgcccccggccacgagaactggcgggaggccttcaccgccgcccatcacatcctgatgtgtcacggcatcgcctcgaacctgcataaggaaaaggggctgaccgggaagatcgggatcaccctcaatatggagcacgtggacgccgccagcgagcgccccgaagacgtcgcggcggcgatccgccgtgatggcttcatcaaccgttggtttgcggaacccctctttaacgggaagtacccggaagatatggtcgagtggtacggcacctacctcaacgggctggactttgtgcagcccggcgatatggaactgatccaacaacccggcgattttctgggcatcaattactacacgcgtagcatcatccggtcgaccaatgacgcgtccctcctccaggtggagcaagtccacatggaggaacccgtcaccgacatgggctgggagatccatcccgagtcgttctataagctcctcacccgcatcgagaaggacttcagcaagggcctccccatcctcatcaccgaaaacggggccgcgatgcgcgatgaggtggtgaacggccagatcgaagacaccggccgccagcgttatatcgaagagcatctgaaggcgtgtcaccgtttcatcgaagagggggggcaactcaaggggtatttcgtctggtcgtttctcgacaactttgagtgggcgtgggggtacagcaagcgcttcgggatcgtgcatatcaattatgaaacgcaagagcggaccccgaagcaatccgccctctggtttaagcaaatgatggccaagaacgggttctaa BBa_K2139006_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagcgagaacacgttcatctttccggcgaccttcatgtgggggacgtcgacgtcctcgtaccagatcgagggcgggacggacgaaggcgggcgcacccccagcatctgggatacgttttgtcaaatccccggcaaggtcatcgggggcgattgcggcgatgtcgcctgcgatcacttccaccactttaaggaggacgtgcagctgatgaagcagctcggctttctgcattaccgtttcagcgtggcctggccgcgcatcatgccggccccgggcatcatcaacgaggaaggcctcctgttctatgagcatctgctcgacgaaatcgaactcgccgggctcatccccatgctcaccctctatcactgggatctcccgcagtggatcgaggatgagggggggtggacgcagcgtgagacgatccagcacttcaagacctacgcgagcgtgatcatggaccgtttcggggaacgcatcaactggtggaacacgatcaacgaaccgtattgcgcctccatcctcgggtatgggacgggggaacatgcccccggccacgagaactggcgggaggccttcaccgccgcccatcacatcctgatgtgtcacggcatcgcctcgaacctgcataaggaaaaggggctgaccgggaagatcgggatcaccctcaatatggagcacgtggacgccgccagcgagcgccccgaagacgtcgcggcggcgatccgccgtgatggcttcatcaaccgttggtttgcggaacccctctttaacgggaagtacccggaagatatggtcgagtggtacggcacctacctcaacgggctggactttgtgcagcccggcgatatggaactgatccaacaacccggcgattttctgggcatcaattactacacgcgtagcatcatccggtcgaccaatgacgcgtccctcctccaggtggagcaagtccacatggaggaacccgtcaccgacatgggctgggagatccatcccgagtcgttctataagctcctcacccgcatcgagaaggacttcagcaagggcctccccatcctcatcaccgaaaacggggccgcgatgcgcgatgaggtggtgaacggccagatcgaagacaccggccgccagcgttatatcgaagagcatctgaaggcgtgtcaccgtttcatcgaagagggggggcaactcaaggggtatttcgtctggtcgtttctcgacaactttgagtgggcgtgggggtacagcaagcgcttcgggatcgtgcatatcaattatgaaacgcaagagcggaccccgaagcaatccgccctctggtttaagcaaatgatggccaagaacgggttctaa BBa_K1813037_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaa BBa_K864400_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z