BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K864400
1
BBa_K864400
Ptac, trp & lac regulated promoter
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
Created from oligos ordered from SigmaAldrich.
Oligo sequences:
Ptac+
AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA
Ptac-
CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
Released HQ 2013
The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]]
[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
false
false
_1124_
0
9827
9
In stock
false
Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
false
Erik Lundin
annotation2197238
1
-10
range2197238
1
29
33
annotation2197237
1
-35
range2197237
1
7
12
annotation2197236
1
Ptac
range2197236
1
1
40
annotation2197240
1
lacO1 site
range2197240
1
36
61
BBa_K1813037
1
BBa_K1813037
ptac and RBS
2015-09-10T11:00:00Z
2015-09-18T07:18:03Z
Registry
ptac RBS
false
false
_2238_
25865
12892
9
false
NA
false
UBC iGEM 2015
component2449457
1
BBa_K864400
component2449459
1
BBa_B0034
annotation2449459
1
BBa_B0034
range2449459
1
70
81
annotation2449457
1
BBa_K864400
range2449457
1
1
61
BBa_K2139003
1
BBa_K2139003
Coding Sequence of E1
2016-10-12T11:00:00Z
2016-10-30T03:51:38Z
Acidothermus cellulolyticus
BBa_K2139003 encompass the coding region for an endo-beta-1,4-glucanase known as E1. E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli.
Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full
false
false
_2609_
33491
34108
9
false
Codon optimized for E. coli
false
UBC iGEM 2016
BBa_K2139007
1
BBa_K2139007
Ptac RBS E1
2016-10-13T11:00:00Z
2016-10-30T03:58:26Z
Acidothermus cellulolyticus
E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli.
Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full
false
false
_2609_
33491
34108
9
false
Codon optimized for E. coli.
false
UBC iGEM 2016
component2527385
1
BBa_K1813037
component2527386
1
BBa_K2139003
annotation2527385
1
BBa_K1813037
range2527385
1
1
81
annotation2527386
1
BBa_K2139003
range2527386
1
88
1236
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2139007_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatggcaggcgggggttattggcatacgagtggccgtgaaatactggacgcaaataatgtgcccgtccgtatcgctgggatcaactggtttgggttcgaaacgtgcaactacgttgttcatggcttgtggagccgcgattatcgcagcatgttagatcagatcaagtccttgggatataatactatacgtcttccctactccgatgatattctgaaacccgggacgatgccaaattctatcaacttttaccagatgaaccaagacctgcaagggttgacctccttgcaagtcatggataagatcgtagcatacgcgggccagatcggacttcgtataatcttagatcgccatagacccgattgtagcggtcagtctgccttgtggtacacaagttcagtatcagaagcaacctggatatctgatttacaagcgcttgcacagcggtataagggaaaccctaccgtcgtgggattcgacttacacaatgagcctcatgacccggcatgttggggctgtggtgacccgtccattgattggcgtttggcagcggaaagagctgggaacgctgtcttatcagtgaatcccaacttgcttatcttcgttgaaggcgttcagtcatacaacggggacagctattggtggggcggcaacctgcaaggcgctggccagtaccccgtagtacttaacgtgccgaacagactggtttattccgcccatgattatgcaacgagcgtctatccacaaacgtggtttagtgaccctacgttcccgaacaatatgccaggtatttggaacaaaaattggggctatctttttaatcagaatatagcgccggtttggttaggtgagtttgggacaacgcttcagtcaaccaccgatcaaacttggctgaaaacattagtacaatatttgcggccgacagcgcagtatggtgcagacagtttccagtggactttctggagttggaatccggactcaggggatactggtgggatcttgaaggacgattggcaaacggtagataccgtgaaagatggttaccttgctccgataaaatcatcgatatttgacccggttggtgcaagcgcaagcccttcctctcaaccctcaccaagtgtatcgccgtcaccgtctccctccccctcataa
BBa_K1813037_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaa
BBa_K2139003_sequence
1
atgcctcgcgctctgcgtcgtgtaccaggcagcagagtaatgttgcgcgttggtgtggtagttgctgttttagcgttggttgcggctttggccaatctggcagtcccacgccctgctcgggccgcaggcgggggttattggcatacgagtggccgtgaaatactggacgcaaataatgtgcccgtccgtatcgctgggatcaactggtttgggttcgaaacgtgcaactacgttgttcatggcttgtggagccgcgattatcgcagcatgttagatcagatcaagtccttgggatataatactatacgtcttccctactccgatgatattctgaaacccgggacgatgccaaattctatcaacttttaccagatgaaccaagacctgcaagggttgacctccttgcaagtcatggataagatcgtagcatacgcgggccagatcggacttcgtataatcttagatcgccatagacccgattgtagcggtcagtctgccttgtggtacacaagttcagtatcagaagcaacctggatatctgatttacaagcgcttgcacagcggtataagggaaaccctaccgtcgtgggattcgacttacacaatgagcctcatgacccggcatgttggggctgtggtgacccgtccattgattggcgtttggcagcggaaagagctgggaacgctgtcttatcagtgaatcccaacttgcttatcttcgttgaaggcgttcagtcatacaacggggacagctattggtggggcggcaacctgcaaggcgctggccagtaccccgtagtacttaacgtgccgaacagactggtttattccgcccatgattatgcaacgagcgtctatccacaaacgtggtttagtgaccctacgttcccgaacaatatgccaggtatttggaacaaaaattggggctatctttttaatcagaatatagcgccggtttggttaggtgagtttgggacaacgcttcagtcaaccaccgatcaaacttggctgaaaacattagtacaatatttgcggccgacagcgcagtatggtgcagacagtttccagtggactttctggagttggaatccggactcaggggatactggtgggatcttgaaggacgattggcaaacggtagataccgtgaaagatggttaccttgctccgataaaatcatcgatatttgacccggttggtgcaagcgcaagcccttcctctcaaccctcaccaagtgtatcgccgtcaccgtctccctccccctcagcctctcgcacaccgacgccgactcccactcccactgcgagccccacacccacattgactcccacagcgaccccaactcccacctag
BBa_K864400_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z