LOCUS       BBa_K2139008            1127 bp    DNA     linear       22-Dec-2024
DEFINITION  G12 Expression Cassette
ACCESSION   BBa_K2139008
VERSION     BBa_K2139008.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     misc_feature    1..81
                     /label=BBa_K1813037
     CDS             88..990
                     /label=BBa_K2139018
     stem_loop       1010..1053
                     /label=stem_loop
     misc_feature    999..1078
                     /label=BBa_B0010
     terminator      999..1078
                     /label=BBa_B0010
     stem_loop       1094..1113
                     /label=T7 TE
     misc_feature    1120..1120
                     /label=stop
     polyA_site      1114..1127
                     /label=polya
     misc_feature    1087..1127
                     /label=BBa_B0012
     terminator      1087..1127
                     /label=BBa_B0012
     terminator      999..1127
                     /label=BBa_B0015
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg ttagtgttaa gagccccacg ctggagagtg
      121 gtcataacgg ctgccgctgt gggcattgcc gccgctggca tcctggttac ttcgcactca
      181 gcttacgggg cgacgacctc cacgtgtagc ccaaccgctg tcgtcagtgt agccggtgat
      241 gagtatcgcg ttcaggcgaa cgaatggaat agctcagctc aacaatgctt gacaatagat
      301 accagtacag gggcgtggtc ggtatccaca gccaatttca atttggccac aaacggcgca
      361 ccggcgacct acccgtctat ttataaggga tgccattggg ggaattgcac gactgccaac
      421 gtaggtatgc ctatacaagt atcaaagatt ggatcggctg taacaagttg gtccacgact
      481 caggtgtcga gcggagctta cgacgtggct tatgacatct ggacaaactc tactcccacc
      541 acatccggac aacccaacgg cactgaggtg atgatttggt tgaactcacg tggcggggta
      601 caaccctttg ggtcccaaac tgcgactggc gtgaccgtcg cgggtcatac gtggaatgta
      661 tggcagggcc aacaaacctc ttggaagata atatcgtacg tattaactcc aggggctacg
      721 tctataagca atctggatct taaggctatc ttagcagatg ctgccgcgcg gggcagcctt
      781 aacacgtcgg actacttaat tgacgtggag gcgggttttg aaatctggca gggtggacag
      841 ggattaggga gcaatagttt ctcggtctcc gttacaagtg gtacctcatc gcctactccc
      901 accccctcac cgtccccttc gccctcaccc gcccctagtc catctcctag cccctctccg
      961 acacctacca gtagtcctac ttcgtcgtag tactagagcc aggcatcaaa taaaacgaaa
     1021 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//